Preparation of nano particles with gold coating iron oxide star-shaped core-shell structure, imaging and thermotherapy application thereof

A technology of iron oxide stars and nanoparticles, which is applied in the direction of nuclear magnetic resonance/magnetic resonance imaging contrast agents, preparations for in vivo tests, medical preparations containing active ingredients, etc., can solve the problem of no gold-coated iron oxide stars. Core-shell structure nanoparticles and other issues, to achieve good MR/CT imaging effect, mild reaction conditions, and good water solubility

Inactive Publication Date: 2014-11-26
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Searching domestic and foreign literature, there is no report on the preparation of gold-coated iron oxide star-shaped core-shell nanoparticles and its application in MR / CT dual-modal imaging and photothermal therapy

Method used

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  • Preparation of nano particles with gold coating iron oxide star-shaped core-shell structure, imaging and thermotherapy application thereof
  • Preparation of nano particles with gold coating iron oxide star-shaped core-shell structure, imaging and thermotherapy application thereof
  • Preparation of nano particles with gold coating iron oxide star-shaped core-shell structure, imaging and thermotherapy application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] (1) Dissolve 0.5g PEI in 10mL water, add AgNO 3 solution (68mg), stirred for 30-60min, then added ice-bathed NaBH 4 Aqueous solution (75.66mg), stirred for 2-4h, dialyzed for 3 days to obtain PEI-Ag nanoparticles. Store at 4°C until use.

[0050] (2) 1.25g FeCl 2 4H 2 O was dissolved in 7.75 mL of ultrapure water, and 6.25 mL of NH 3 ·H 2 O and stirred under air atmosphere for 10 minutes, then transferred the mixed solution to the autoclave, and added the PEI-Ag aqueous solution prepared in (1) to the autoclave, stirred and mixed evenly, and reacted at 134°C 3 hours. After the reaction, cool down to room temperature naturally, wash and purify the precipitate by magnetic separation, and obtain ferric iron tetroxide nanoparticles Fe with silver seeds. 3 o 4 -PEI-Ag. Store at 4°C until use.

[0051] (3) Dissolve 381mg cetyltrimethylammonium bromide (CTAB) in 10mL water, add 580μL HAuCl 4 solution (30mg / mL), stirred for 10 minutes and added 1.1mg AgNO 3 , after ...

Embodiment 2

[0056] Get the Fe prepared by Example 1 3 o 4 / Au-HA NSs nanoparticle aqueous solution 5 μL, and then make 100 μL nanoparticle suspension with ultrapure water. Drop 5 μL of the nanoparticle suspension on the surface of the copper grid, and dry it in the air for TEM testing (see attached Figure 4 ). TEM results showed that Fe 3 o 4 The morphology of / Au-HA NSs is star-shaped, and the particle size is about 120nm. High-resolution TEM shows that the surface of the nanoparticles is covered with an organic layer.

Embodiment 3

[0058] The Fe of Example 1 3 o 4 / Au-HA NSs The concentration of Fe element in the solution was measured by ICP-OES test method, and then 2 mL of aqueous solutions with Fe concentrations of 0.005, 0.01, 0.02, 0.04 and 0.08 mM were prepared in EP tubes with ultrapure water, and the magnetic resonance Imaging determination of the imaging effect and T of the material at different Fe concentrations 2 relaxation effect (see attached Figure 5 ). T 2 Weighted imaging results show that the Fe prepared by the present invention 3 o 4 / Au-HA NSs The MR signal intensity decreased with the increase of iron concentration. The relaxation rate test results show that Fe 3 o 4 The reciprocal relaxation time of / Au-HA NSs has a good linear relationship with the increase of iron concentration (in the concentration range of 0.0025-0.04mM). And the Fe prepared by the present invention can be obtained by calculation 3 o 4 r of Au-HA NSs 2 Relaxation rate is 144.39mM -1 the s -1 . The...

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Abstract

The invention relates to a preparation of nano particles with gold coating iron oxide star-shaped core-shell structure, imaging and thermotherapy application thereof. The preparation method comprises the following steps: preparing PEI coating silver nano seed; synthesizing iron oxide nano particles having silver seed by a one-step hydrothermal method; preparing the star-shaped composite nano particles in a gold growth solution; and modifying polyethyleneimine (PEI) and hyaluronic acid (HA) on the surface of the star-shaped composite nano particles. The preparation has the advantages of mild reaction condition, simple technology and easy operation. The prepared nano particles with the gold coating iron oxide star-shaped core shell structure has good molecular imaging performance and thermotherapy antineoplastic effect, and has latent application value on in vivo tumour imaging diagnosis and photo-thermal treatment fields.

Description

technical field [0001] The invention belongs to the field of preparation of diagnostic and therapeutic agents, in particular to the preparation of a gold-coated iron oxide star-shaped core-shell nanoparticle and its application in imaging and thermotherapy. Background technique [0002] Malignant tumors have been the number one killer of human life for a long time, with the characteristics of high mortality, difficult treatment and rapid deterioration. Therefore, early diagnosis and treatment of tumors is particularly important. At present, there are various detection methods for tumors, mainly including ultrasound imaging, CT imaging, nuclear medicine PET imaging and magnetic resonance imaging (MRI). With the development of nuclear magnetic resonance (MR) and computed tomography (CT), their scanning time for lesions has gradually shortened, their resolution has gradually improved, and their detection has become more accurate. main means. At the same time, in order to imp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/12A61K49/08A61K49/04A61K41/00A61P35/00
Inventor 沈明武李静超胡勇韦平史向阳
Owner DONGHUA UNIV
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