Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Suppository used for treating cancer

A technology for suppositories and cervical cancer, applied in the application of drugs, in the field of suppositories, can solve the problems of limited drug concentration, toxic and side effects, and low bioavailability

Inactive Publication Date: 2014-12-03
JIANGSU TASLY DIYI PHARMA CO LTD
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Chemotherapy drugs are usually administered intravenously or orally. However, due to the unstable nature of temozolomide, it is easy to degrade into small molecules with large polarity in the intestinal fluid with alkaline pH, which is not conducive to absorption and directly leads to drug absorption. Poor, low bioavailability, so oral anticancer effect is poor
Secondly, intravenous administration must first prepare the drug into an aqueous solution, but it is obviously not suitable for temozolomide with poor water solubility, and more importantly, temozolomide is degraded in a neutral aqueous solution, so stability will also become an important issue for intravenous administration. question
In addition, systemic administration of chemotherapy drugs often causes serious side effects, such as bone marrow suppression, gastrointestinal toxicity, damage to liver, kidney, and heart, etc., which are often unbearable for patients, and systemic administration can achieve local tumorigenesis. Drug concentration is also very limited

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Suppository used for treating cancer
  • Suppository used for treating cancer
  • Suppository used for treating cancer

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0052] In vitro killing activity of temozolomide API on tumor cells

[0053] Materials and Methods

[0054] Test substance: temozolomide, Tianjin Tasly Group Co., Ltd.

[0055] Positive control drug: temozolomide (raw material drug), Jiangsu Tasly Diyi Pharmaceutical Co., Ltd.

[0056] Drug preparation method: before use, weigh the raw material drug powder, dissolve it with dimethyl sulfoxide (DMSO), and dilute it with cell culture medium to the concentration required for the experiment.

[0057] Cell lines: Human cervical cancer cells ME180 and MS751 were obtained from ATCC. Human colon cancer cell line HT-29 and human cervical cancer cell line Hela are all cell lines preserved in our laboratory.

[0058] Reagents: RPMI1640 is a product of GIBCO; MTT is a product of Bebco; calf serum was purchased from Hangzhou Sijiqing Bioengineering Materials Co., Ltd.

[0059] Instrument: BIORAD550 microplate reader.

[0060] Experimental method: tetrazolium salt [3-(4,5-dimethylthiaz...

experiment example 2

[0076] Inhibitory effect of temozolomide smear on human cervical carcinoma MS751 subcutaneously transplanted tumor in nude mice

[0077] Basic Experimental Process

[0078] Tumor-bearing animals with good tumor growth and good general condition were selected and sacrificed by cervical dislocation. The tumor mass was taken out under aseptic conditions, cut into a tumor mass with a diameter of 2-3 mm with a scalpel, and inoculated subcutaneously in the axilla of nude mice with a trocar. Or grind the tumor tissue and pass it through a 100-mesh sieve to prepare a cell suspension in a volume of 3×10 7 / 0.1ml / only inoculated subcutaneously in nude mice. A few days later, the average volume of tumors in tumor-bearing mice reached 100mm 3 In the above cases, the animals were randomly divided into stratified groups according to the tumor volume, and the animals in each group were given drugs according to the experimental plan. Wherein the negative control group tumor grows naturall...

experiment example 3

[0117] Inhibitory effect of temozolomide smear on human cervical cancer HELA subcutaneous xenografts

[0118] Basic experimental method is the same as experimental example 2

[0119] Materials and Methods

[0120] Experimental sample: temozolomide ointment, the content is 2.5%, 5.0%, 10.0%

[0121] Control drug: doxorubicin hydrochloride (doxorubicin) for injection, a product of Pharmacia Italia S.p.A, converted to the intraperitoneal injection of the animal according to the drug instruction manual

[0122] Animals: BALB / c nu mice, female, 5-6 weeks old

[0123] Model: nude mice bearing cervical cancer HELA tumor

[0124] experiment procedure:

[0125] Seven days after the inoculation of the tumor block, the tumor volume of the tumor-bearing mice reached an average of 100 mm 3 Above, according to tumor volume size random stratification grouping. A negative control group was set up; temozolomide 2.5%, 5% and 10% three content groups and a blank dosage form control group; ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Volumeaaaaaaaaaa
Login to View More

Abstract

The invention relates to applications of temozolomide or a pharmaceutical composition containing the temozolomide in preparation of medicines for treating cervical cancer, colorectal cancer, and other diseases. The composition comprises the temozolomide and a pharmaceutically acceptable carrier thereof. The preparation formula is a suppository preferably. The invention also relates to a formula of the suppository and a preparing method of the suppository.

Description

technical field [0001] The invention belongs to the field of pharmacy, and specifically relates to the application of temozolomide or a pharmaceutical composition containing temozolomide in the preparation of medicines for treating diseases such as cervical cancer and colorectal cancer. The pharmaceutical composition includes temozolomide and pharmaceutically acceptable The carrier, the preferred preparation form is a suppository, the formula composition and preparation method of the suppository. Background technique [0002] Temozolomide is an alkylating anticancer drug with broad-spectrum antitumor activity, especially for glioma (brain cancer) and melanoma (skin cancer). The trade name is Temodal, and the dosage forms on the market are capsules and injections. However, since temozolomide cannot pass through artificial skin (silicon membrane), rat skin and human skin, it cannot be directly made into a transdermal preparation (WO00 / 57867), the application range of temozolo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K31/4188A61K9/02A61P35/00A61P1/00A61P15/00
Inventor 李德馨陈莹王国成王永峰
Owner JIANGSU TASLY DIYI PHARMA CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com