Staphylococcus aureus chromogenic culture media

Abstract

The invention belongs to the field of microorganisms and in particular relates to a staphylococcus aureus chromogenic culture media. The culture media comprises the following components: a trisaccharide iron (TSI) agar culture media, 12 g / L casein peptone, 6 g / L mannitol, 2.0 g / L dipotassium hydrogen phosphate, 0.5 g / L potassium dihydrogen phosphate, 8 g / L glycine, 12 g / L sodium pyruvate and a 0.16 g / L color developing agent, wherein the pH value is 7.2-7.4. According to the staphylococcus aureus chromogenic culture media, 1 L of the TSI agar culture media contains 15.0 g of tryptone, 10.0 g of soybean peptone, 5.0 g of yeast powder, 4.0 g of sodium chloride, 10.0 g of sucrose, 0.2 g of ferrous sulfate, 0.3 g of sodium thiosulfate, 15.0 g of agar, 0.04 g of bromcresol purple and the balance of distilled water. The staphylococcus aureus chromogenic culture media is simple in component, convenient to prepare, low in cost and favorable for popularization and application; staphylococcus aurei are high in growth speed, low in aberration rate and convenient to observe and analyze.

Description

technical field [0001] The invention belongs to the field of microorganisms, in particular to a chromogenic culture medium for Staphylococcus aureus. Background technique [0002] The diversity and variability among microorganisms in the world and the ecological complexity of species habitats have brought about the breadth, complexity and permanence of exploring and understanding microorganisms. A species of microorganism is a unique gene pool. The metabolism of microorganisms is an intricate life activity. In the long-term biological evolution, a complete and unified sensitive regulatory system has been formed. Depending on this regulatory system, various metabolic activities are strictly controlled. . Methodical and coordinated and adapts flexibly to circumstances. Different culture media, due to different media formulations, affect its growth and variation. [0003] Medium is an artificially prepared nutrient for the growth and maintenance of microorganisms, plants, an...

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Problems solved by technology

[0005] At present, it is still not possible to infer and calculate the medium formula suitable for a certain strain from the basic principles of biochemical reactions. We can only use the basic theories of biochemistry, cell biology, microbiology, etc., and refer to the more suitable ones used by predecessors. The empirical formula of a certain type of strain, combined with the characteristics of the strains used and the product, uses small fermentation equipment such as shake flasks and glass tanks, and selects a more suitable medium according to certain experimental designs and experimental methods