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Revertose detection method

A detection method, glucose technology, applied in the detection field, can solve the problems of large consumption of standard sugar solution, many interference factors, high cost of use, etc., and achieve the effect of fast operation, simple and safe operation, and low consumption

Inactive Publication Date: 2014-12-17
梁山正大菱花生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing film reagent method needs to titrate the sample in a boiled state, which is difficult to operate, has many interference factors, consumes a lot of standard sugar solution, and costs a lot.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 3. Preparation of reagents:

[0031] (1) Liquid A: Weigh 60g of copper sulfate (CuSO4·5H2O), fully dissolve it with distilled water, and dilute to 1L;

[0032] (2) Liquid B: Weigh 300g of potassium sodium tartrate (KNaC4H4O6·4 H2O) and 50g of sodium hydroxide, fully dissolve it with distilled water and dilute to 1L;

[0033] (3) Liquid C: Weigh 200g of potassium iodide, fully dissolve it with distilled water, and dilute to 1L;

[0034] (4) Liquid D: Measure 100ml of 98% concentrated sulfuric acid, add it to distilled water, mix well, and cool to a constant volume to 1L;

[0035] (5) E solution: Weigh 20g (or 16g anhydrous sodium thiosulfate) of AR grade sodium thiosulfate (Na2S2O3·5H2O), add 0.1g sodium carbonate in a beaker, fully dissolve it with distilled water, and dilute to 1L;

[0036] (6) Starch indicator (2%): Weigh 1.0g of soluble starch, dissolve it with 100ml of boiled distilled water, and add a little salicylic acid for preservative;

[0037] (7) Glucose standard soluti...

Embodiment 2

[0054] 1. Preparation of reagents:

[0055] (1) Liquid A: Weigh copper sulfate (CuSO 4 ·5H 2 O) 69.2g, fully dissolved in distilled water, diluted to 1L;

[0056] (2) Liquid B: Weigh potassium sodium tartrate (KNaC 4 H 4 O 6 ·4 H 2 O) 346g and 100g sodium hydroxide, fully dissolved and diluted to 1L with distilled water;

[0057] (3) Liquid C: Weigh 300g of potassium iodide, fully dissolve it with distilled water, and dilute to 1L;

[0058] (4) Liquid D: Measure 142ml of concentrated sulfuric acid with a content of 98%, add it to distilled water, mix well, and cool to a constant volume to 1L;

[0059] (5) E liquid: Weigh AR grade sodium thiosulfate (Na 2 S 2 O 3 ·5H 2 O) 26g (or 16g anhydrous sodium thiosulfate), add 0.2g sodium carbonate to a beaker, fully dissolve it with distilled water, and dilute to 1L;

[0060] (6) Starch indicator (2%): Weigh 2.0g of soluble starch and dissolve it with 100ml of boiled distilled water. Add a little salicylic acid for preservative.

[0061] (7) Gluco...

Embodiment 3

[0078] 5. Preparation of reagents:

[0079] (1) Liquid A: Weigh 80g of copper sulfate (CuSO4·5H2O), fully dissolve it with distilled water, and dilute to 1L;

[0080] (2) Liquid B: Weigh 400g of potassium sodium tartrate (KNaC4H4O6·4 H2O) and 150.0g of sodium hydroxide, fully dissolve it with distilled water and dilute to 1L;

[0081] (3) Liquid C: Weigh 400g of potassium iodide, fully dissolve it with distilled water, and dilute to 1L;

[0082] (4) Liquid D: Measure 200ml of 98% concentrated sulfuric acid, add it to distilled water, mix well, and cool to a constant volume to 1L;

[0083] (5) Liquid E: Weigh 30g (or 16g anhydrous sodium thiosulfate) of AR grade sodium thiosulfate (Na2S2O3·5H2O), add 0.3g sodium carbonate to a beaker, fully dissolve it with distilled water, and dilute to 1L;

[0084] (6) Starch indicator (2%): Weigh 3.0g of soluble starch, dissolve it with 100ml of boiled distilled water, and add a little salicylic acid for preservation;

[0085] (7) Glucose standard solut...

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Abstract

The invention discloses a revertose detection method, comprising the following steps: sequentially adding 10-15ml of liquid A, 10-15ml of liquid B and 0.5-1.5ml of a sample solution (glucose equivalent is 30-80mg) to a triangular flask of 250ml; supplementing the triangular flask with distilled water until the total volume of the liquid in the triangular flask is 50ml; heating and boiling for 2-5 minutes, and quickly cooling to room temperature; adding 5-15ml of liquid C and 5-15ml of liquid D, and titrating liquid E, when the end point is approached, namely the color is faint yellow, adding 2-3ml of 12% starch indicator, and when the tested solution becomes purple, continuing to titrate, so that the tested solution becomes pink from purple, thereby reaching the end point. The revertose detection method has the beneficial effects that thermal titration is not needed, and the method is simple and safe to operate, low in consumption of glucose standard solution, fast in operation and accurate in data.

Description

technical field [0001] The invention belongs to the technical field of detection, and more specifically relates to a method for detecting reducing sugar content in a fermentation process. Background technique [0002] Reducing sugar is the most basic industrial raw material in the fields of fermentation and food. The rapid and accurate determination of reducing sugar is of great significance for production process control and food quality inspection. At present, the commonly used detection method is the film reagent method. The divalent copper ions in the reducing sugar and CuSO4 in Fehling's reagent are reduced to monovalent copper, and the end point of the reaction is indicated by methylene blue. According to the amount of reducing sugar required for a certain amount of Fehling's reagent to be completely reduced, the added The reducing sugar content in the sample. However, the existing film reagent method needs to titrate the sample in a boiled state, which is difficult t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N31/16
Inventor 李兆博邵明丽
Owner 梁山正大菱花生物科技有限公司
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