Preparation method of probiotic-rich pleurotus eryngii dietary fiber
A technology of dietary fiber and probiotics, applied in the direction of food preparation, bacteria and bifidobacteria used in food preparation, etc., to achieve the effects of low production cost, convenient operation and few influencing factors
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Embodiment 1
[0023] (1) Take by weighing 100kg Pleurotus eryngii slag after extracting the polysaccharide, add 200L, concentration is 75% edible alcohol, stir 1 hour while soaking, select 250 mesh screens to filter and remove protein after soaking;
[0024] (2) Transfer the mushroom dregs after the protein removal in step (1) to the hydrolysis tank, add 200L of water, cover the mushroom dregs, then add 3 moles of sodium hydroxide solution per liter, adjust the pH to 8.5, and heat up to 60°C, alkali hydrolysis for 3 hours, filter after alkali hydrolysis, and wash with water until neutral;
[0025] (3) Put the mushroom dregs that have been hydrolyzed by alkali in step (2) into the hydrolysis tank, add 300L of water to cover, then add 2 moles per liter of hydrochloric acid solution, adjust the pH to 3.5, heat up to 50°C, and acid hydrolyze for 4 hours , filter after the acid hydrolysis is completed, and wash to neutral to obtain crude dietary fiber; mix the acid hydrolysis wastewater and alka...
Embodiment 2
[0030] (1) Take by weighing 150kg Pleurotus eryngii slag after extracting polysaccharide, add 300L, concentration is 95% edible alcohol, stir 1 hour while soaking, select 250 mesh screens to filter and remove albumen after soaking finishes;
[0031] (2) Transfer the mushroom dregs after protein removal in step (1) to a hydrolysis tank, add 300 L of water, cover the mushroom dregs, then add 3 moles of potassium hydroxide solution per liter, adjust the pH to 7.0, and heat up to 50 ℃, alkali hydrolysis for 5 hours, after alkali hydrolysis, filter and wash with water until neutral;
[0032] (3) Put the mushroom dregs that have been hydrolyzed by alkali in step (2) into the hydrolysis tank, add 450L of water to cover, then add 2 moles per liter of sulfuric acid solution, adjust the pH to 2.0, heat up to 40°C, and acid hydrolyze for 2 hours , acid hydrolysis is completed and filtered, and washed to neutrality to obtain the crude product of Pleurotus eryngii dietary fiber; at the sam...
Embodiment 3
[0037] (1) take by weighing 100kg of Pleurotus eryngii slag after extracting polysaccharide, be 90% edible alcohol with 200L concentration, stir while soaking for 3 hours, select 250 mesh sieves to filter and remove albumen after soaking finishes;
[0038] (2) Transfer the mushroom residue after protein removal in step (1) to a hydrolysis tank, add 200L of water, and submerge it, then add 3 moles of ammonia water per liter, adjust the pH to 10.0, heat up to 65°C, and perform alkaline hydrolysis After 1 hour, alkali hydrolysis is completed, filtered, and washed with water until neutral;
[0039] (3) Put the mushroom dregs that have been hydrolyzed by alkali in step (2) into the hydrolysis tank, add 300L of water to soak, then add 2 moles of phosphoric acid solution per liter, adjust the pH to 6.0, heat up to 55°C, and acid hydrolyze for 8 hours , filter after the acid hydrolysis, and wash to neutrality to obtain the crude Pleurotus eryngii dietary fiber; at the same time, mix t...
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