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A method for separating polymethoxylated flavonoids, hesperidin and synephrine in Citrus aurantium

A technology of polymethoxylated flavones and hesperidin, which is applied in the extraction of active ingredients of traditional Chinese medicine, hesperidin and synephrine, and the separation of polymethoxylated flavones in Citrus aurantium, can solve the problem of polymethoxylated flavones and polymethoxylated flavones. Solve the problems of separate separation of hesperidin, complicated process and high cost, and achieve the effect of low cost, improved economic value and simple method

Inactive Publication Date: 2016-09-21
CHENGDU LYUTUO BIOLOGICAL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to solve the problem that the simultaneous extraction of active ingredients in Fructus Citrus Citrus Fructus requires multiple methods, complex process, low efficiency, high cost, and the isolated flavonoids are still mixtures, and polymethoxylated flavones and polymethoxylated flavonoids cannot be combined. To solve the problem of separating out hesperidin alone, a method for step-by-step extraction of polymethoxylated flavones, hesperidin and synephrine by using macroporous adsorption resin is proposed. Separation of oxyflavones and hesperidin

Method used

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  • A method for separating polymethoxylated flavonoids, hesperidin and synephrine in Citrus aurantium

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Embodiment 1

[0033] A method for separating polymethoxylated flavones, hesperidin and synephrine in Citrus aurantium, comprising the following steps:

[0034] a. Macroporous adsorption resin pretreatment

[0035] After soaking the macroporous adsorption resin in 90% ethanol for 20 hours, wash it with water, then soak it in 5% hydrochloric acid for 8 hours, wash it with water until it is neutral, then soak it in 5% sodium hydroxide solution for 5 hours, and wash it with 5% sodium hydroxide solution for 5 hours. After washing with water to neutrality, fill it with wet method, then wash the resin with ethanol until the eluate is clear, wash with water and set aside;

[0036] b. Citrus aurantium pretreatment

[0037] After crushing the dried Fructus Fructus Fructus Fructus Fructus Fructus Fructus Aurantii, add the ethanol with a weight volume ratio of 1:9-11 and a concentration of 60-80% to Fructus Fructus Fructus Fructus Fructus Fructus Fructus Powder, reflux extraction 2-4 times, 5-7 hours ...

Embodiment 2

[0045] A method for separating polymethoxylated flavones, hesperidin and synephrine in Citrus aurantium, comprising the following steps:

[0046] a. Macroporous adsorption resin pretreatment

[0047] After soaking the macroporous adsorption resin in 95% ethanol for 24 hours, wash it with water, then soak it in 6% hydrochloric acid for 5 hours, wash it with water until it is neutral, then soak it in 7% sodium hydroxide solution for 8 hours, and use After washing with water to neutrality, fill it with wet method, then wash the resin with ethanol until the eluate is clear, wash with water and set aside;

[0048] b. Citrus aurantium pretreatment

[0049] After 500g of dried Fructus Fructus Citrus Fructus is pulverized, adding and Fructus Fructus Fructus Fructus Fructus Weight Volume ratio is 1: 11, concentration is the ethanol of 80%, reflux extracts 4 times, each 7h, after filtering, multiple filtrates are combined, concentrated To a concentration of 35%, adjust the pH value to...

Embodiment 3

[0057] A method for separating polymethoxylated flavones, hesperidin and synephrine in Citrus aurantium, comprising the following steps:

[0058] a. Macroporous adsorption resin pretreatment

[0059] After soaking the macroporous adsorption resin in 95% ethanol for 23 hours, wash it with water, then soak it in 6% hydrochloric acid for 6 hours, wash it with water until neutral, then soak it in 5% sodium hydroxide solution for 6 hours, and wash it with After washing with water to neutrality, fill it with wet method, then wash the resin with ethanol until the eluate is clear, wash with water and set aside;

[0060] b. Citrus aurantium pretreatment

[0061] After the dried 500g of Fructus Fructus Fructus Citrus Fructus is crushed, add ethanol with a weight volume ratio of 1:10 and a concentration of 70% to Fructus Fructus Fructus Fructus Fructus Fructus Fructus Fructus Fructus Fructus Fructus Powder, and extract by reflux 4 times, each time for 7h, after filtering, combine multip...

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Abstract

The invention discloses a method for separating polymethoxylated flavones, hesperidin and synephrine from immature bitter orange. Macroporous ion exchange resin is adopted and subjected to five steps including macroporous adsorption resin pretreatment, immature bitter orange pretreatment, polymethoxylated flavones extraction, hesperidin extraction and synephrine extraction, and the three effective components in the immature bitter orange are extracted step by step. The method is simple in technological process, high in efficiency and capable of separating polymethoxylated flavones and hesperidin from flavone, and has the advantages of low cost, high efficiency and sufficient use of resources.

Description

Technical field [0001] The invention relates to a method for extracting active ingredients of traditional Chinese medicine, in particular to a method for separating polymethoxyflavonoids, hesperidin and synephrine from Fructus Aurantii, and belongs to the field of traditional Chinese medicine processing. Background technique [0002] Citrus aurantium is the dried young fruit of Yun spice lime and its cultivated variety sweet orange. Its nature is bitter, pungent, sour, and warm. It returns to the spleen and stomach meridian. It has the effects of breaking Qi, eliminating accumulation, resolving phlegm and dispersing pimples. Citrus aurantium mainly contains brass, volatile oils and a small amount of alkaloids. Flavonoids not only have antibacterial, antiviral, anti-inflammatory, and vasodilation effects, but also inhibit lipid peroxidation, platelet aggregation, and the activity of a large number of enzymes, and can improve the permeability and fragility of capillaries; amon...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/30C07D311/40C07H17/07C07H1/08C07C215/60C07C213/10
CPCC07C213/10C07D311/30C07D311/40C07H1/08C07H17/07C07C215/60
Inventor 刘健
Owner CHENGDU LYUTUO BIOLOGICAL SCI & TECH CO LTD