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Cultivation and preparation method of feed probiotic

A technology of prebiotics and feed, applied in the field of prebiotics, can solve the problems of large amount of addition, many miscellaneous bacteria and high cost, and achieve the effects of low cost, high bacterial amount and promoting the growth of livestock and poultry.

Inactive Publication Date: 2014-12-24
山东文远生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the amount of bacteria in the product obtained by fermentation and purification treatment of a single strain is less than 10 billion cfu / g, and there are many miscellaneous bacteria, and the amount of input into the livestock and poultry breeding industry is relatively large, resulting in high costs and certain economic losses for farmers. burden

Method used

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  • Cultivation and preparation method of feed probiotic
  • Cultivation and preparation method of feed probiotic

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preparation example Construction

[0015] A method for cultivating and preparing feed prebiotics of the present invention comprises the following steps:

[0016] A microbial strain is selected, and the selected microbial strain is Bacillus subtilis or Bacillus licheniformis.

[0017] Cultivate the selected microbial strains, including:

[0018] (1) Eggplant bottle slant medium culture, 1% tryptone, 0.5% yeast extract, 1% sodium chloride, 1.5% agar powder, 121 ° C, sterilized for 30 minutes; make a slant, and spread it after cooling. Incubate at 38°C for 18h.

[0019] (2) Primary seed tank culture, soybean meal 1%, corn flour 1%, glucose 0.01%, corn steep liquor 0.5%, sulfate 0.001%, peptone 0.001%, sodium hydroxide 0.001%, defoamer 0.001%; ​​121℃ Sterilize for 30 minutes, cool down to 38°C, and aseptically connect the prepared strains to the seed tank at a temperature of 38°C, a tank pressure of 0.05Mpa, a speed of 160 rpm, and an air volume of 160m 3 / h, cultivated for 10h.

[0020] (3) Secondary seed tank...

Embodiment 1

[0024] Cultivation and preparation method of Bacillus subtilis feed probiotics

[0025] 1. Strain culture

[0026] Add 1% tryptone, 0.5% yeast extract, 1% sodium chloride, 1.5% agar powder, sterilize at 121°C for 30 minutes to make a slant medium, add 1% tryptone, 0.5% yeast extract, 1% sodium chloride, and sterilize for 30 minutes to make a slant medium for eggplant bottle. After cooling, spread and inoculate with 38 Cultivate at ℃ for 18-24h.

[0027] 2. Seed culture

[0028] The bacterial classification cultivated in the eggplant bottle is transferred to the 500ml triangular flask in a sterile manner, in order to inoculate the seed tank. Add 4% soybean meal, 2% corn flour, and 0.03% protease to 400L primary seed tank culture medium, and hydrolyze with 45°C for 40 minutes, then add 0.6% glucose, 1.2% corn steep liquor, 0.025% manganese sulfate, and 0.075% sodium hydroxide , defoamer 0.16%, sterilized at 121°C for 30 minutes, cooled to 38°C, aseptically connected the prepa...

Embodiment 2

[0035] Bacillus subtilis, Bacillus licheniformis (10702)

[0036] 1. Strain culture

[0037] Add 1% tryptone, 0.5% yeast extract, 1% sodium chloride, 1.5% agar powder, sterilize at 121°C for 30 minutes to make a slant medium, add 1% tryptone, 0.5% yeast extract, 1% sodium chloride, and sterilize for 30 minutes to make a slant medium for eggplant bottle. After cooling, spread and inoculate with 38 Cultivate at ℃ for 18-24h.

[0038] 2. Seed culture

[0039] The bacterial classification cultivated in the eggplant bottle is transferred to the 500ml triangular flask in a sterile manner, in order to inoculate the seed tank. Add 4% soybean meal, 2.5% corn flour, and 0.03% protease to 400L primary seed tank medium, and hydrolyze with 45°C for 40 minutes, then add 0.6% glucose, 0.025% manganese sulfate, 0.03% sodium hydroxide, and 0.16% defoamer %, sterilize at 121°C for 30 minutes, cool down to 38°C, aseptically transfer the prepared strains into the seed tank at a temperature of ...

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Abstract

The invention discloses a cultivation and preparation method of feed probiotic. The method comprises the following steps: 1) selecting a microorganism strain which is either bacillus subtilis or bacillus licheniformis; and 2) cultivating the selected microorganism strain: (1) slant culture medium cultivation through an eggplant bottle; (2) cultivation through a primary seeding tank; (3) cultivation through a secondary seeding tank; and (4) fermenting cultivation. A method of powdering a finished product comprises the following steps: adding 0.1-3% of disodium hydrogen phosphate and 0.1-3% of calcium chloride which serve as a flocculating agent as well as calcium carbonate which functions as a filter aid to fermentation liquor, stirring for 20-30min at 60 DEG C, carrying out plate frame filtering, collecting solids, and drying the solids in a cyclone separator to obtain a powdery solid product. Bacterium amount of the feed probiotic prepared by the preparation method disclosed by the invention is equal to or more than 50 billion cfu / g, and the feed probiotic is high in activity, wide in application, reasonable in price and completely meets demands of various farmers.

Description

technical field [0001] The invention relates to a preparation method of a feed probiotic, in particular to a method for obtaining a prebiotic after selecting bacterial strains through seed cultivation, fermentation and purification treatment. Background technique [0002] Probiotics, also known as probiotics, probiotics, live bacteria preparations, and microecological regulators, have no uniform regulations on their Chinese names, and their interpretations are not consistent. First of all, Lilley and Stillwell used the term probiotic in 1965, which is opposite to antibiotic, and the two have contradictory effects. Antibiotics directly kill or inhibit the growth of harmful bacteria. In 1974, RE Parker of the United States believed that 'prebiotics are microorganisms or substances that maintain the balance of intestinal flora'. In 1989, Fuller redefined it as 'a live microbial feed additive that can exert beneficial effects on animals by changing the balance of intestinal mi...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/10C12R1/125A23K1/00A23K1/16
Inventor 李国文赵洪涛袁伟岗
Owner 山东文远生物技术有限公司