Isaria fumosorosea oil suspension
A technology of rose-smoke-colored spores and oil suspension, which can be used in insecticides, biocides, animal repellents, etc., and can solve the problem of uneven distribution of active ingredients, high requirements for spray equipment, and unsuitable promotion and popularization. problem, to achieve the effects of good stability, simple preparation method and improved control effect
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Embodiment 1-22
[0015] Example 1-22, the following table 1, take the components of various contents, first add the rose smoke-colored tofuspore spore powder to the vegetable oil stirred at a constant speed in the mixer, so that the spore powder is evenly distributed in the vegetable oil, and the processing process The ambient temperature is not higher than 40°C; at the same time, mix the emulsifier and stabilizer evenly in the grinder; finally mix the above two mixtures in the grinder, grind and mix evenly at a speed of 1000-1500rpm, the environment during processing The temperature is not higher than 40°C, so that the oil suspension of I. fumigatus is obtained.
[0016] Table 1 prepares the used components and content (percentage by weight) of the rose fume-colored I. japonicus oil suspension
[0017]
[0018]
Embodiment 23
[0019] Example 23, the control effect of I. fumigatus oil suspension on Bemisia tabaci
[0020] With 0.5% Tween 80 aqueous solution, the content is 5×10 6 spores / ml of spore suspension. Get the fourth instar Bemisia tabaci as the insect for testing, routinely spray after diluting the oil-suspension of I. tabaci in different times with water obtained in Example 4, and set the clear water (CKO) contrast and 5 × 10 at the same time. 6 Spores / ml of spore suspension (CK1) control. Adjust the temperature of the light incubator to 25±2°C and the relative humidity to 50±5%, observe regularly every day, record the number of dead insects in each treatment, and calculate the corrected mortality rate 7 days after application.
[0021] Table 2 The control effect of the conventional spray of I. tabaci oil suspension on Bemisia tabaci
[0022]
[0023] It can be seen from Table 2 that the corrected mortality rates on the 7th day after application were 98.0, 95.4, and 83.3, respectively...
Embodiment 24
[0024] Example 24, the control effect of I. fumigatus oil suspension on cabbage aphid
[0025] In 2013, a field efficacy test of one-time use fungi was conducted on vegetable aphids that harm eggplants in vegetable greenhouses in the suburbs of Nanjing. The oil suspension prepared in Example 13 was diluted 1000 times with water and carried out constant manual spraying. The amount of bacteria used in the diluent is: the amount of spore oil suspension contained in it reaches 20ml / mu. The relative control effect was 67.3% on the 6th day after using the bacteria, and 99.5% on the 10th day, while the population density of the cabbage aphid in the control treatment increased by 3.5 and 6.1 times respectively compared with that before using the bacteria.
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