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Fuchsin basic sodium sulfite agar and application thereof

A technology of red sodium sulfite and sodium sulfite, applied in the field of magenta sodium sulfite agar, can solve problems such as inability to infer and calculate, and achieve the effects of low cost, low variation rate and simple formula

Inactive Publication Date: 2015-01-07
QINGDAO KANGHEWEIYE COMML
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, it is still not possible to infer and calculate the medium formula suitable for a certain strain from the basic principles of biochemical reactions. We can only use the basic theories of biochemistry, cell biology, microbiology, etc., and refer to the more suitable ones used by predecessors. The empirical formula of a certain type of strain, combined with the characteristics of the strains used and the product, uses small fermentation equipment such as shake flasks and glass tanks, and selects a more suitable medium according to certain experimental designs and experimental methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A fuchsin sodium sulfite agar, the components of the culture medium include EC broth, 5.0g / L beef powder, 5.0g / L yeast powder, 1.0g / L basic fuchsin, 6.0g / L sodium sulfite, 15.0g / L agar, the pH value is 7.2-7.4;

[0023] On the basis of the above scheme, the EC broth contains 20.0 g of tryptone, 10.0 g of lactose, 2.0 g of potassium dihydrogen phosphate, 5.0 g of sodium chloride, and 4.0 g of dipotassium hydrogen phosphate per liter of EC broth. for distilled water.

[0024] Weigh 52.5g of this product, add 20ml of absolute ethanol and 980ml of distilled water, heat and stir to dissolve, subpackage, autoclave at 116°C for 20 minutes, and set aside.

[0025] Incubate at 36±1°C for 18-24 hours

[0026] Quality Control Strains

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PUM

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Abstract

The invention relates to the field of microorganisms, and particularly relates to fuchsin basic sodium sulfite agar. A culture medium comprises the following components: EC broth, 5.0g / L of beef powder, 5.0g / L of yeast powder, 1.0g / L of basic fuchsin, 6.0g / L of sodium sulfite and 15.0g / L of agar; the pH value is 7.2-7.4; and each liter of EC broth contains 20.0g of tryptone, 10.0g of lactose, 2.0g of monopotassium phosphate, 5.0g of sodium chloride, 4.0g of dipotassium phosphate and the balance of distilled water. The culture medium disclosed by the invention is simple in component, convenient to prepare and operate, low in cost, and beneficial for popularization and application; and bacteria are rapid in growth, low in aberration rate, and convenient to observe and analyze.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a magenta sodium sulfite agar and an application thereof. Background technique [0002] The diversity and variability among microorganisms in the world and the ecological complexity of species habitats have brought about the breadth, complexity and permanence of exploring and understanding microorganisms. A species of microorganism is a unique gene pool. The metabolism of microorganisms is an intricate life activity. In the long-term biological evolution, a complete and unified sensitive regulatory system has been formed. Depending on this regulatory system, various metabolic activities are strictly controlled. . Methodical and coordinated and adapts flexibly to circumstances. Different culture media, due to different media formulations, affect its growth and variation. [0003] Medium is an artificially prepared nutrient for the growth and maintenance of microorganis...

Claims

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Application Information

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IPC IPC(8): C12Q1/10C12N1/20
Inventor 高玉成王玉芝
Owner QINGDAO KANGHEWEIYE COMML
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