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Method for extracting oil from chlorella

A chlorella and oil technology, which is applied in the production of fat/fat and fat production, can solve the problems of troublesome operation, high energy consumption, complicated process, large time consumption, energy consumption, etc., to reduce energy consumption, reduce technological process, The effect of reducing material loss

Inactive Publication Date: 2015-01-28
GANSU DEFU BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This process consumes a lot of time and energy
In addition, during the process of recovering molecular sieves, molecular sieves and algal cells are mixed together, which will cause loss of algal cells or blockage of molecular sieves.
In the process of molecular sieve regeneration, heating or decompression is required to restore the dehydration performance of molecular sieves. This process also consumes a lot of energy, and the overall cost is high, which is not conducive to popularization and factory production.
[0005] Chinese patent CN102559376B discloses a method for extracting neutral oil from heterotrophic chlorella, but this method needs to collect algae cells by centrifugation first, and consumes more energy during the whole centrifugation process
Use of centrifugation will result in loss of fat from dead or autolyzed fractions
[0006] Chinese patent 201210431210.7 discloses a bio-oil in algae cells and its preparation method and application. This method uses ozone and high pressure to treat algae cells together, and continuously suppresses and releases pressure for 40 to 80 times to achieve the effect of cell crushing. This process is cumbersome to operate and consumes a lot of energy, and there is a lot of noise when the pressure is released quickly.
[0007] Chinese patent CN103725413A discloses a method for extracting seaweed oil from Chlorella. This method uses repeated freeze-thaw technology to break the wall of Chlorella, and uses ethanol as a solvent to extract seaweed oil. The operation is simple and the energy consumption is small, but the broken Poor wall effect and low oil extraction rate
[0008] In summary, the existing disclosed algae oil extraction technologies all have the disadvantages of complex process, high energy consumption, low extraction rate, and slow extraction rate.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1) Cell disruption: Mix fresh chlorella with phosphate buffer solution to prepare 100 mL of chlorella solution with a concentration of 1×10 10 Algae cells / mL, then add 100 mg of cellulase with an activity unit of 200,000, and then insert the ultrasonic probe of a 250W ultrasonic breaker into the chlorella solution at a distance of 1 cm from the bottom, at 25-35°C, pH 6.0 Under ambient conditions for 1 hour of cell disruption, the cell disruption rate was 96.3%;

[0029] 2) Extraction: Add 50 mL of hydrophobic organic solvent petroleum ether 2 (boiling range 60-90° C.) to the crushed chlorella solution in the above step 1), and extract the oil and fat components for 1 hour at a temperature of 30-40° C. The process oscillates intermittently;

[0030] 3) Preparation of algae oil and recovery of chlorella cells: After the extraction, add 10% potassium hydroxide solution to precipitate chlorella cells for recovery, shake well and stand still for 10 minutes, collect the uppe...

Embodiment 2

[0032] 1) Cell disruption: Mix fresh chlorella with phosphate buffer solution to prepare 100 mL of chlorella solution with a concentration of 1×10 10 Algae cells / mL, then add 300mg of cellulase with an activity unit of 200,000, and then insert the ultrasonic probe of a 250W ultrasonic breaker into the chlorella solution at a distance of 1 cm from the bottom, at 25-35°C, pH 6.0 Cell disruption was carried out under ambient conditions for 1 hour, and the measured cell disruption rate was 99.2%;

[0033] 2) Extraction: Add 50 mL of hydrophobic organic solvent petroleum ether 2 (boiling range 60-90° C.) to the crushed chlorella solution in the above step 1), and extract the oil and fat components for 1 hour at a temperature of 30-40° C. The process oscillates intermittently;

[0034] 3) Preparation of algae oil and recovery of chlorella cells: After the extraction, add 10% potassium hydroxide solution to precipitate chlorella cells for recovery, shake well and stand still for 10 ...

Embodiment 3

[0036] 1) Cell disruption: Mix fresh chlorella with phosphate buffered saline solution to prepare 100 mL of chlorella solution with a concentration of 1×10 10 Algae cells / mL, then add 500mg of cellulase with an activity unit of 200,000, and then insert the ultrasonic probe of a 250W ultrasonic breaker into the chlorella solution at a distance of 1 cm from the bottom, at 25-35°C, pH 6.0 Cell disruption was performed for 1 hour under ambient conditions, and the measured cell disruption rate was 99.5%;

[0037] 2) Extraction: Add 50 mL of hydrophobic organic solvent petroleum ether 2 (boiling range 60-90° C.) to the crushed chlorella solution in the above step 1), and extract the oil and fat components for 1 hour at a temperature of 30-40° C. The process oscillates intermittently;

[0038] 3) Preparation of algae oil and recovery of chlorella cells: After the extraction, add 10% potassium hydroxide solution to precipitate chlorella cells for recovery, shake well and stand still ...

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Abstract

The invention discloses a method for extracting oil from chlorella and belongs to the technical field of biochemical engineering of microalgae. The method comprises steps of cell discruption and extraction, preparation of algae oil and recovery of chlorella cells. According to the method, no flocculant is required to be added, no concentration is required, algae preparation powder is not required to be centrifuged or dried, and the method has the advantages that the method has a short technical process of extracting the algae oil, is simple in operation process, short in extraction time, low in energy consumption and cost and high in algae oil yield, and is suitable for directly extracting the oil from fermentation liquid.

Description

technical field [0001] The invention belongs to the technical field of microalgae biochemical engineering, and in particular relates to a method for extracting oil from chlorella. Background technique [0002] Global warming and the oil crisis have given birth to the development of a low-carbon economy, and the research on alternative energy has become urgent. As a new type of green energy, microalgae biodiesel can reduce CO 2 The advantages of low emission, high lipid content, high yield, and small land occupation area have become the research hotspots of countries all over the world. [0003] Utilizing chlorella for oil production is a complex system engineering, covering multiple technical links, including screening and cultivation of chlorella, large-scale cultivation of chlorella, collection and processing of oil and so on. Each link has higher requirements on equipment and production level. The cell wall of microalgae is tough, and the cell body is smaller than that...

Claims

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Application Information

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IPC IPC(8): C11B1/10C11B1/04
CPCC11B1/10C11B1/025
Inventor 白玉龙张国威
Owner GANSU DEFU BIOTECH
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