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Dual-functionalized graphene oxide composite material as well as preparation method and application thereof

A composite material, graphene technology, applied in the direction of chemical reaction of materials for analysis, chemiluminescence/bioluminescence, etc., can solve problems such as limitations, and achieve the effect of improving luminous intensity, improving stability, and expanding the scope of application

Active Publication Date: 2015-01-28
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this N-(4-aminobutyl)-N-ethyl isoluminol light-emitting functionalized graphene material synthesized in the prior art needs to exhibit excellent chemiluminescent properties under alkaline conditions, which To a certain extent, its application in biomedicine, biosensing and other fields is limited
In addition, although the luminescent functionalized graphene oxide composite material has high analytical sensitivity, there is still room for improvement in its sensitivity.

Method used

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  • Dual-functionalized graphene oxide composite material as well as preparation method and application thereof
  • Dual-functionalized graphene oxide composite material as well as preparation method and application thereof
  • Dual-functionalized graphene oxide composite material as well as preparation method and application thereof

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preparation example Construction

[0064] B. Preparation method of bifunctional graphene oxide composite material

[0065] The invention also provides a simple and rapid green method for preparing the bifunctional graphite oxide composite material of the invention.

[0066] In one embodiment, the reaction of the luminol-based chemiluminescence reagent of general formula (I) of the present invention and the graphene oxide aqueous solution is preferably carried out under stirring conditions, so as to obtain the graphene oxide of primary luminescent reagent monofunctionalization composite material. The reaction conditions are not particularly limited, and may be normal temperature and pressure. Preferably, the pH of the reaction system is about 8-12, preferably about 9-11, more preferably about 11. The mass ratio of the luminol-based chemiluminescence reagent of general formula (I) to graphene oxide is 1:100-1:20, preferably 1:50-1:10, more preferably 1:40.

[0067] In one embodiment of the present invention, t...

Embodiment 1

[0092] Example 1: Preparation of horseradish peroxidase and N-(4-aminobutyl)-N-ethylisoluminol bifunctional graphene oxide composite material 1 (Material 1)

[0093] In this embodiment, the luminol-based chemiluminescent reagent is N-(4-aminobutyl)-N-ethyl isoluminol, and the biological substance containing ferrous protoporphyrin active center is horseradish peroxide enzyme. The preparation method is as follows, wherein steps (b) and (c) are as follows Figure 9 Shown:

[0094] (a) At room temperature, a certain amount of graphite oxide was dissolved in an appropriate amount of ultrapure water, and ultrasonically stripped for 50 minutes to obtain a graphene oxide aqueous solution; the obtained graphene oxide aqueous solution was centrifuged at 4000 rpm for 5 minutes , and then remove the precipitate to obtain a pure graphene oxide aqueous solution;

[0095] (b) Under stirring at room temperature, a solution of 0.01 mol / liter of N-(4-aminobutyl)-N-ethyl isoluminol in 0.1 m...

Embodiment 2

[0099] Example 2: Preparation of horseradish peroxidase and luminol bifunctional graphene oxide composite material 2 (Material 2)

[0100] According to the same method for preparing material 1 in Example 1, except that luminol was used instead of N-(4-aminobutyl)-N-ethylisoluminol in step (b), material 2 was obtained . According to the above-mentioned test method (6), the chemiluminescent intensity of material 2 was measured, and the results of the maximum light intensity are shown in Table 1.

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Abstract

The invention relates to a dual-functionalized graphene oxide composite material as well as a preparation method and application thereof. The dual-functionalized graphene oxide composite material comprises graphene oxide, a light emitting fragment derived from a luminal chemiluminescent reagent shown as a formula (I) (in the Specification) and a light emission enhancing fragment derived from a biological substance containing a ferrous protoporphyrin active center, wherein the light emitting fragment derived from the luminal chemiluminescent reagent shown as the formula (I) and the light emission enhancing fragment derived from the biological substance containing the ferrous protoporphyrin active center are bound on the surface of the graphene oxide. The dual-functionalized graphene oxide composite material has an excellent light-emitting property.

Description

technical field [0001] The invention relates to a bifunctional graphene oxide composite material containing a chemiluminescence segment and a luminescence enhancement segment. The invention also relates to the preparation and application of the bifunctional graphene oxide composite material. Background technique [0002] Graphene oxide has attracted much attention in recent years because of its excellent mechanical, optical and electrical properties, special structure and easy preparation. Graphene oxide has a large surface area, abundant oxygen-containing functional groups on the surface, and good water solubility, making it an ideal choice for the synthesis of new functional composite materials. [0003] At present, functional molecules are usually bound to the surface of graphene oxide through covalent bonds and non-covalent bonds, so as to endow functionalized composites with corresponding properties. For example, in the prior art, N-(4-aminobutyl)-N-ethyl isoluminol w...

Claims

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Application Information

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IPC IPC(8): G01N21/76
Inventor 崔华刘晓英
Owner UNIV OF SCI & TECH OF CHINA
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