Edible fungus stock culture medium and preparation method thereof

Abstract

The invention relates to the technical field of preparation of edible fungus media, in particular to an edible fungus stock culture medium and a preparation method thereof. The stock culture medium is prepared by combining potatoes, glucose, agar powder and water, and the potatoes are unpeeled, so that kinds of raw materials of the prepared edible fungus stock culture medium are less than those of a traditional edible fungus stock culture medium, however, nutritional ingredients of the stock culture medium can meet the growth requirement of edible fungus stock cultures, the unpeeled potatoes bring partial fiber materials to the medium, and the structure of the stock culture medium is improved; two times of hardening and tempering, PH adjustment and raw material treatment in preparation technology steps are combined, so that ingredients of all the raw materials have complex micro biochemical reactions, the required nutritional structure is produced directionally, the nutritional structure of the stock culture medium is richer, the nutritional environment better meets the growth requirement of the stock cultures, the growth rate of the stock cultures is increased, the culture cycle is shortened, and the culture cost of the edible fungus stock cultures is reduced.

Description

technical field [0001] The invention relates to the technical field of edible fungus culture medium production, in particular to an edible fungus parent culture medium and a preparation method thereof. Background technique [0002] In the prior art, there are a variety of cultivation techniques for edible fungi, among which the most common and popular edible fungus cultivation scheme is to adopt a multi-stage strain culture method, that is, to prepare the mother seed medium first to carry out the mother seed cultivation. Cultivation, after obtaining the mother seed bacterium liquid, put the bacterium liquid on the original seed medium for original seed culture to obtain the original seed bacterium liquid; then place the original seed bacterium liquid on the cultivar medium for cultivation to obtain the cultivar , and put the cultivated species into the fruiting bag or the culture box, and control and adjust the process parameters appropriately to obtain the edible fungus; W...

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Problems solved by technology

[0003] The mother seed medium is the link that can provide basic strains for the subsequent cultivation steps during the cultivation of edible fungi. Therefore, the quality and quality of the mother seed cultivated at this stage will affect the cultivation obtained in the entire cultivation technology. Therefore, in the multi-stage cultivation technology of edible fungi, the research and exploration of the parent culture medium has become an essential part of the multi-stage cultivation of edible fungi, and it is also a key step in the cultivation of edible fungi. Therefore, a large number of researchers have also made a lot of research since then, such as the patent No. CN201410035566.8 "A Kind of Edible Fungus Mother Culture Medium and Its Preparation Method" through peeled potatoes, hard wood chips, glucose , sucrose, potassium dihydrogen phosphate, magnesium sulfate, gibberellin, 2,4-D, agar and water, prepared by weighing and adjusting the pH, then adding agar components, putting them into test tubes, and adjusting the content of liquid medium in the test tubes After plugging cotton plugs, carry out autoclave and carry out autoclaving after autoclaving, obtain the culture medium of mother species; Another example is " a kind of culture medium for cultivating edible fungus to produce mother species " with patent No. CN201210263132.4 through potato juice, glucose , Potassium dihydrogen phosphate, magnesium sulfate, agar powder, fish meal wastewater concentrate and the remaining water are added to the edible fungus production parent medium by replacing peptone with fish meal wastewater concentrate, which improves the quality and growth rate of mycelium growth. It is prepared by combining preparation, mixing, adding agar, heating and dissolving, and subpackaging; however, the above-mentioned medium is still difficult to achieve an ideal level for the cultivation of the mother species, and cannot make the appearance of the mother species hyphae beautiful , slow growth rate, long culture cycle, high culture cost