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Preparation method of freeze-dried powder for modifying ixtle SOD

A technology of agave hemp and freeze-dried powder, which is applied in the direction of biochemical equipment and methods, enzymes, oxidoreductases, etc., can solve the problems of high production cost, not necessarily able to achieve the desired effect, and insufficient stability of the product, and achieve immunity The effect of reducing or eliminating the originality, shortening the activation time, and improving the activation efficiency

Active Publication Date: 2015-02-18
LINGNAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although its two patents use small molecules to modify SOD molecules, the absorption and utilization rate of the human body is improved, but the stability of the product is not high enough, which limits its application to a certain extent, and there is a problem of high preparation cost
Zhao Hua's "Preliminary Study on Superoxide Dismutase Modified by Polyethylene Glycol" (Advances in Modern Biomedicine, 2008, 8 (02): 277-279) activated polyethylene glycol (PEG) with a molecular weight of 6000Da as the modification agent, to covalently modify corn superoxide dismutase (SOD), PEG-6000 is activated by cyanuric chloride, but this method may not be able to achieve the desired effect when used in other plant SOD substances

Method used

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  • Preparation method of freeze-dried powder for modifying ixtle SOD
  • Preparation method of freeze-dried powder for modifying ixtle SOD
  • Preparation method of freeze-dried powder for modifying ixtle SOD

Examples

Experimental program
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Effect test

Embodiment 1

[0024] A. Microwave Assisted Activation of Monomethoxypolyethylene Glycol (mPEG) Synthesis of Activation Modifiers:

[0025] Mix 100 parts by mass of mPEG, 3 parts by mass of SA, and 80 parts by mass of DMF; place the mixture in a microwave reflux device, select a power of 10%, and microwave for 3 minutes, then stop the radiation; Ethyl ether or petroleum ether to make the precipitation complete; filter with suction, discard the filtrate and keep the precipitate; add DMF to the obtained precipitate with stirring until the precipitate is completely dissolved, filter with suction, discard the precipitate and keep the filtrate; add anhydrous ether to the obtained filtrate to make After the precipitation is complete, filter with suction, discard the filtrate and keep the precipitate; repeat the above operation 3 times to obtain the intermediate product I, namely monomethoxypolyethylene glycol succinate (mPEG-S);

[0026] In a hot bath at 40°C, add 3 parts by mass of NHS, 80 parts...

Embodiment 2

[0030] A. Microwave Assisted Activation of Monomethoxypolyethylene Glycol (mPEG) Synthesis of Activation Modifiers:

[0031] Mix 120 parts of mass mPEG, 6 parts of mass SA, and 120 parts of mass DMF; place the mixture in a microwave oven reflux device, select a power of 90%, microwave radiation for 60 minutes, and stop the radiation; wait for the mixture to cool to room temperature, and then add Ethyl ether or petroleum ether to make the precipitation complete; filter with suction, discard the filtrate and keep the precipitate; add dichloromethane to the obtained precipitate with stirring until the precipitate is completely dissolved, filter with suction, discard the precipitate and keep the filtrate; add petroleum ether to the obtained filtrate Make the precipitation complete, filter with suction, discard the filtrate and keep the precipitate; repeat the above operation 5 times to obtain the intermediate product I, namely monomethoxypolyethylene glycol succinate (mPEG-S);

...

Embodiment 3

[0036] A. Microwave Assisted Activation of Monomethoxypolyethylene Glycol (mPEG) Synthesis of Activation Modifiers:

[0037] Mix 110 parts by mass of mPEG, 5 parts by mass of SA, and 90 parts by mass of DMF; place the mixture in a microwave oven reflux device, select a power of 70%, microwave radiation for 50 minutes, and stop the radiation; when the mixture is cooled to room temperature, add oil under stirring Add ether to make the precipitation complete; filter with suction, discard the filtrate and keep the precipitate; add chloroform to the obtained precipitate under stirring until the precipitate is completely dissolved, filter with suction, discard the precipitate and keep the filtrate; add anhydrous ether to the obtained filtrate to precipitate complete, filter with suction, discard the filtrate and keep the precipitate; repeat the above operation 4 times to obtain the intermediate product I, namely monomethoxypolyethylene glycol succinate (mPEG-S);

[0038] In a heat ...

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Abstract

The invention relates to a preparation method of freeze-dried powder for modifying ixtle SOD and belongs to the technical field of organic synthesis and application. The preparation method is characterized in that microwave-assisted succinic anhydride (SA) and N-hydroxyl succinimide (NHS) activated monomethoxypolyethylene glycol (Mpeg) are used as a activation modifying agent and ixtle superoxide dismutase (SOD) is used as a modifying object to prepare a modifier for modifying ixtle SOD. According to the preparation method of the activation modifying agent, microwave-assisted heating is utilized, so that the activation time is shortened greatly, and the activation efficiency is improved; the ixtle SOD is modified by the activation modifying agent, and the modifier for modifying SOD is prepared; the obtained modifier has higher capability of heat resistance, acid-base resistance and proteolysis resistance compared with natural ixtle superoxide dismutase, and the immunogenicity is lowered or eliminated.

Description

technical field [0001] The invention relates to a preparation method of plant SOD freeze-dried powder, in particular to a preparation method of modified agave hemp SOD freeze-dried powder, which belongs to the technical field of organic synthesis and application. Background technique [0002] Superoxide dismutase (SOD) is a kind of metalloenzyme widely present in organisms, and copper (Cu), zinc (Zn), iron (Fe), manganese (Mn) and other trace elements (tace elements) Metabolism is closely related. SOD is an important oxygen free radical scavenger in the organism, which can balance the oxygen free radicals in the body, so as to avoid the adverse reactions caused when the concentration of superoxide anion free radicals in the body is too high. It has shown unique functions in inhibiting tumors and cancers, autoimmune therapy, etc., and has attracted extensive attention from scholars and researchers at home and abroad. The research fields have involved many disciplines such as...

Claims

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Application Information

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IPC IPC(8): C12N9/02
CPCC12N9/0089C12Y115/01001
Inventor 陈冰
Owner LINGNAN NORMAL UNIV
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