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Application of zebra fish in detection of reproduction toxicity of BBP (Butyl Benzyl Phthalate) and method for rapidly detecting reproduction toxicity of BBP

A reproductive toxicity, zebrafish technology, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of high sample purity, poor sensitivity, and uncertain detection indicators.

Inactive Publication Date: 2015-02-25
ANHUI ACADEMY OF ENVRIONMENTAL SCIENCE RESEARCH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009]In toxicology research, the choice of experimental model has always been a difficult problem. Common animal models such as rats and mice have a long growth cycle (more than 8 weeks) , the detection index is uncertain, the sensitivity is poor, the sample purity is high and it is difficult to study on a large scale, and the pure cell material is difficult to reproduce the actual situation in the body

Method used

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  • Application of zebra fish in detection of reproduction toxicity of BBP (Butyl Benzyl Phthalate) and method for rapidly detecting reproduction toxicity of BBP
  • Application of zebra fish in detection of reproduction toxicity of BBP (Butyl Benzyl Phthalate) and method for rapidly detecting reproduction toxicity of BBP
  • Application of zebra fish in detection of reproduction toxicity of BBP (Butyl Benzyl Phthalate) and method for rapidly detecting reproduction toxicity of BBP

Examples

Experimental program
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Embodiment 1

[0026] 1. Breeding of zebrafish: 1) Wild-type zebrafish of the AB line were purchased from the International Zebrafish Center of Oregon State University (Oregon State University, OR. USA). Adult zebrafish were housed in 2.5 L aquariums with circulating system water passing through them. Adult zebrafish were fed brine shrimp twice a day. The brine shrimp are hatched from synthetic seawater. From 2 to 16 days after birth, the juvenile fish were fed with artificial feed Hatchfry EncapsulonTM (Argent Chemical Laboratories, Redmond, WA, USA). The constant temperature of the fish room is 28.5 oC, and the time of lighting and turning off the lights is 14:10 hours; 2) The tap water is filtered by activated carbon and treated by a reverse osmosis device to obtain purified water, and then natural salt of analytical purity is added to it to synthesize water that meets the requirements of the US Environmental Protection Agency. Standard (U.S. Environmental Protection Agency Guideline, U...

Embodiment 2

[0034] In order to further understand the effects of BBP exposure on the reproductive function of female and male fish, we designed a permutation mating experiment with control female / male fish respectively.

[0035] a, raising of zebrafish: with embodiment 1.

[0036] b. Detecting the acute toxic effect of BBP on zebrafish for 7 days: the same as in Example 1.

[0037] c. The reproductive toxicity of BBP was detected in the female / male fish replacement mating experiment: BBP treatment group (BBP concentrations were 0.6 mg.L -1 ,0.9 mg.L -1 ,1.2 mg.L -1 ,1.5mg.L -1 ) each randomly selected 18 males and each randomly selected 18 females. Eighteen males from each BBP treatment group were removed and replaced with 18 males from the solvent control group (BBP concentration 0), and mated with the corresponding females of the BBP treatment group. The same substitution was also applied to the females of each BBP treatment group. At 8 o'clock the next day, collect fish roe stati...

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Abstract

The invention belongs to the technical field of biology, relates to a detection method for detecting reproduction toxicity of BBP (Butyl Benzyl Phthalate), in particular relates to a detection method for detecting the reproduction toxicity of BBP by utilizing model organism zebra fishes and specifically relates to a method for rapidly detecting the male sperm production capability and female egg production capability of BBP by utilizing adult zebra fishes. According to the method disclosed by the invention, the experimental result displays that the detection period is only 8 days and is shorter than that of other methods reported in the existing literature; and moreover, the lowest concentration of the detected reproduction toxicity of BBP is 0.6mg.L<-1> and is superior to the detected concentration in other methods.

Description

technical field [0001] The invention discloses the application of zebrafish in detecting the reproductive toxicity of BBP and a method for rapidly detecting the reproductive toxicity of BBP. Background technique [0002] 1) BBP [0003] Phthalates (PAEs) are a class of compounds widely used as plasticizers in polyvinyl chloride. In recent years, it has been found that it is also a class of potential environmental endocrine disruptors. As a plasticizer, since PAEs and the main structure of the plastic are not combined with chemical bonds, they will be continuously released from the plastic during use and pollute the environment. The US Center for Human Reproductive Risk Evaluation (CERHR) proposed 9 PAEs closely related to human health through comprehensive assessment, and BBP is one of them. The current methods for detecting BBP plasticizers are GC-MS and liquid chromatography-mass spectrometry (HPLC-MS). Although the method can basically meet the requirements of routine...

Claims

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Application Information

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IPC IPC(8): G01N33/00
Inventor 豆长明张洁齐翠翠潘成荣王军杨远盛聂天琛
Owner ANHUI ACADEMY OF ENVRIONMENTAL SCIENCE RESEARCH
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