Microbial preparation for purifying sewage and deodorizing garbage and preparation method thereof

[0022] The preparation method of the composite strain is divided into two steps, one-stage and two-stage.

[0023] The above-mentioned "first level" is the pure culture of each component. Pick the colonies of each microorganism, respectively inoculate them into 100-200mL liquid culture medium, place them in a constant temperature shaker at 30-35℃, 100-200r/min for 16-24h; but Lactobacillus acidophilus and Lactobacillus plantarum do not shake , Stand still and cultivate. The inoculation amount of each microorganism is basically the same.

[0024] The above-mentioned "secondary" is mixed culture, the specific method is: prepare 5-10L liquid medium, inoculate the culture of Saccharomyces cerevisiae, Hansenula yeast, Rhodopseudomonas palustris, and Streptomyces tenuifolia after the first-level culture, 30~ 32 ℃ intermittent stirring (60~120r/min mechanical stirring, once every 3~5h, 1~2min each time) for 12~24h; then inoculate Bacillus licheniformis, Bacillus subtilis, Bacillus megaterium, Lactobacillus acidophilus, plant Lactobacillus, Streptococcus thermophilus, continue to cultivate for 16-24 hours. After incubation, the smelly ones are regarded as contaminated bacteria and should be discarded; those with sour smell are normal.

[0025] The fermentation culture process is divided into three stages: initial fermentation, high-sugar fermentation, and silent fermentation. The conditions are as follows:

[0026] Initial fermentation

[0027] Pump the prepared composite strain into the seed tank containing the above liquid culture medium, cultivate it naturally (natural temperature 15~30℃) without any conditions for 24~48h, and then pump it into a quarter volume liquid culture Based on the fermenter, the culture volume in the fermentor is about half of the total volume of the tank. In the fermenter at 30-32℃, pH lower limit 4.2, stirring for 1 min every 8-12h, provide light (for example, high-pressure gas discharge lamp, such as metal halide lamp, xenon lamp, etc., irradiate the liquid surface on the upper part of the fermenter, The role is to promote the growth of Rhodopseudomonas palustris and become the dominant bacteria, usually the illumination is not less than 1000LUX;), cultivate until floating matter (like fungus moss) appears. Usually the fermentation time at this stage is 1 to 3 days.

[0028] High sugar fermentation

[0029] Pump into the fermenter and then add 2 to 5% molasses the liquid medium to approximately three-quarters of the volume, and stir evenly. At 28~30℃, pH lower limit 3.9, stirring for 1 min every 8~12h, continue to provide light, cultivate until the pH no longer drops significantly. Usually the fermentation time at this stage is 4-8 days.

[0030] Silent fermentation

[0031] No longer heating, stirring, and light. When the pH lower limit is 3.9 and the pH upper limit is 4.4, continue to cultivate. When the pH value is lower than the lower limit, add sodium hydroxide or sodium bicarbonate to adjust; when the pH value is higher than the upper limit, add Adjust with sulfuric acid or glacial acetic acid. Most of the floating objects (more than 80%) sink, and the color is bright red or orange-yellow, the smell is sour or ester, and the number of viable bacteria reaches 1×10 10 Cfu/mL or more can be put in the can. Usually the fermentation time at this stage is 5-10 days.

[0036] Example 1

[0037] 1.1 Preparation of liquid culture medium

[0038] Take 8% molasses, 2% salt, 1% urea, and 2% compound growth factor according to the mass ratio, and add 5 tons of medium with dechlorinated tap water. Among them, 2 tons are put into 3 tons of seed tanks, 2.5 tons are put into 10 tons of fermentation tanks, and the remaining 0.5 tons are reserved.

[0039] 1.2 Preparation of composite strains

[0040] Dispense the spare liquid culture medium into several 200 mL Erlenmeyer flasks, each 100 mL, and autoclave at 121°C for 15 min. Afterwards, in the ultra-clean workbench, use an inoculating loop to pick some colonies from the test tube slope of each component, inoculate them into each bottle of culture medium, and place them in a constant temperature shaker at 32°C, 150r/min for 24h, but they are fond of Lactobacillus acidi and Lactobacillus plantarum are cultivated in a static state.

[0041] Take 10L of spare liquid medium, inoculate Saccharomyces cerevisiae, Hansenula, Rhodopseudomonas palustris, Streptomyces flavus, and cultivate for 24 hours at 32°C. During the cultivation period, use an impeller stirring motor to stir for one minute every 4 hours at a stirring rate of 60r/ min. After inoculation with Bacillus licheniformis, Bacillus subtilis, Bacillus megaterium, Lactobacillus acidophilus, Lactobacillus plantarum, Streptococcus thermophilus, and after sufficient stirring, continue to stand for 24 hours.

[0042] 1.3 Initial fermentation

[0043] The prepared composite strain is pumped into the seed tank, and after 48 hours of natural cultivation without conditions, pumped into the fermentation tank. In a fermenter at 32°C, pH lower limit 4.2, stirring for 1 min every 8h, providing light (using a metal halide lamp, projected from the top to the liquid surface, intensity 1000LUX), culture for 2 days until floating matter appears.

[0044] 1.4 High-sugar fermentation

[0045] Pump the freshly prepared liquid medium and 5% molasses (based on the volume of the newly added medium) into the fermenter to about three-quarters of the volume, and stir evenly. Stir for 1 min every 12h at 28°C and the lower limit of pH 3.9, continue to provide light, and continue to cultivate for 4 days until the pH no longer drops significantly.

[0046] 1.5 Silent fermentation

[0047] Without heating, stirring, and lighting, under the conditions of controlling the lower limit of pH 3.9 and the upper limit of pH 4.4, continue to cultivate until 80% of the floating matter sinks, the color of the fermentation system is bright red or orange, and the smell is sour or estery. Stir the fermentation broth and sample for colony count at time, if the number of viable bacteria reaches 1×10 10 Cfu/mL or more can be put in the can.

[0048] Finally, about 8 tons of this microecological preparation was obtained.

[0049] Example 2

[0050] 2.1 Preparation of liquid culture medium

[0051] Take 8% molasses, 2% salt, 1% urea, and 2% compound growth factor according to the mass ratio, and add 10 tons of medium with dechlorinated tap water. Among them, 4 tons are put into seed tanks, 5 tons are put into 20 tons fermentation tanks, and the remaining 1 ton is reserved.

[0052] 2.2 Preparation of composite strains

[0053] Dispense the spare liquid culture medium into several 200 mL Erlenmeyer flasks, each 100 mL, and autoclave at 121°C for 15 min. Then in the ultra-clean workbench, use an inoculating loop to pick some colonies from the test tube seed slope of each component, inoculate them into each bottle of culture medium, and place it in a constant temperature shaker at 32°C, 150r/min for 24h, acidophilic Lactobacillus and Lactobacillus plantarum are cultivated in a static state. Take 10L of spare liquid medium, inoculate Saccharomyces cerevisiae, Hansenula, Rhodopseudomonas palustris, Streptomyces gracilis, and cultivate at 32°C for 24 hours. During the cultivation period, use an impeller stirring motor to stir for one minute every 4 hours at a stirring rate of 60r/ min. Then inoculate Bacillus licheniformis, Bacillus subtilis, Bacillus megaterium, Lactobacillus acidophilus, Lactobacillus plantarum, Streptococcus thermophilus and stir well, and continue to stand for 24 hours.

[0054] 2.3 Initial fermentation

[0055] The prepared composite strain is pumped into the seed tank, and after 48 hours of natural cultivation without conditions, pumped into the fermentation tank. In a fermenter at 32°C, pH lower limit 4.2, stirring for 1 min every 8h, providing light (using a metal halide lamp, projected from above to the liquid surface, intensity of about 2000 LUX), and incubating for another 3 days until floating matter appears.

[0056] 2.4 High-sugar fermentation

[0057] Pump the freshly prepared liquid medium and 5% molasses based on the new medium to about three-quarters of the volume into the fermentor, and stir evenly. Stir for 1 min every 12h at 28°C and the lower limit of pH 3.9, provide light, and continue to cultivate for 5 days until the pH no longer drops significantly.

[0058] 2.5 Silent fermentation

[0059] No heating, stirring, light, the lower limit of pH 3.9, the upper limit of pH 4.4, culture until 80% of the floating matter sinks, the color of the culture is bright red or orange, the smell is sour or ester, and the number of viable bacteria reaches 1×10 10 Cfu/mL or more can be put in the can.

[0060] Finally, about 17.5 tons of this microecological preparation was obtained.

[0061] Usage and effect

[0062] (1) Dilute the microecological preparation obtained in Example 1 by 200 times and spray it evenly in a garbage transfer station with a knapsack sprayer. The deodorizing effect is significant. The concentration changes of ammonia and hydrogen sulfide are shown in the following table:

[0063]

[0064] (2) The microecological preparation obtained in Example 2 was added to the aeration tank of the sewage treatment system of a food company at an amount of 0.1% of the sewage influent, once a day, and the effect was ideal. The water quality changes as shown in the table below Indication: (Dosing of microecological preparations will start on August 14)

[0065] sampling time