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Microbial preparation for purifying sewage and deodorizing garbage and preparation method thereof

A technology for micro-ecological preparations and sewage purification, which is applied in microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., and can solve the problems of short duration of action, long activation time, and poor treatment effect. , to achieve the effect of environmental improvement

Inactive Publication Date: 2015-03-04
上海寄绿生物环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some existing micro-ecological preparations for water treatment have achieved some treatment effects, they generally have problems such as long activation time, short duration of action, and single type of microorganisms, which lead to poor treatment effects and other problems.

Method used

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  • Microbial preparation for purifying sewage and deodorizing garbage and preparation method thereof
  • Microbial preparation for purifying sewage and deodorizing garbage and preparation method thereof
  • Microbial preparation for purifying sewage and deodorizing garbage and preparation method thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0022] The preparation method of the composite bacteria is divided into two steps, one step and two steps.

[0023] The above "level one" refers to the pure culture of each component. Pick the colonies of each microorganism, inoculate them into 100-200mL liquid medium, place them in a constant temperature shaker at 30-35°C, 100-200r / min and cultivate them for 16-24 hours; however, Lactobacillus acidophilus and Lactobacillus plantarum do not shake , static cultivation. The inoculation amount of each microorganism was basically the same.

[0024] The above "secondary" is a mixed culture. The specific method is: prepare 5-10L liquid medium, inoculate the cultures of S. Intermittent stirring (60-120r / min mechanical stirring, once every 3-5h, 1-2min each time) at 32°C for 12-24h; after that, inoculate Bacillus licheniformis, Bacillus subtilis, Bacillus megaterium, Lactobacillus acidophilus, plant For Lactobacillus and Streptococcus thermophilus, continue to culture for 16-24 hou...

Embodiment 1

[0037] 1.1 Preparation of liquid medium

[0038] Take 8% molasses, 2% salt, 1% urea, and 2% compound growth factors according to the mass ratio, add dechlorinated tap water to prepare 5 tons of medium. Among them, 2 tons are put into 3-ton seed tanks, 2.5 tons are put into 10-ton fermentation tanks, and the remaining 0.5 tons are reserved.

[0039] 1.2 Preparation of composite strains

[0040] The spare liquid culture medium was divided into several 200mL Erlenmeyer flasks, 100mL in each bottle, and sterilized by high-pressure steam at 121°C for 15min. Finally, in the ultra-clean workbench, use an inoculation loop to pick some colonies from the slant of the test tubes of each component, inoculate them into each bottle of culture medium, and place them in a constant temperature shaker at 32°C and 150r / min for 24 hours. Lactobacillus acidum and Lactobacillus plantarum were cultured statically.

[0041] Take 10L of spare liquid medium, inoculate Saccharomyces cerevisiae, Hanse...

Embodiment 2

[0050] 2.1 Preparation of liquid medium

[0051] Take 8% molasses, 2% salt, 1% urea, and 2% compound growth factors according to the mass ratio, and add dechlorinated tap water to prepare 10 tons of medium. Among them, 4 tons are put into seed tanks, 5 tons are put into 20-ton fermentation tanks, and the remaining 1 ton is reserved.

[0052] 2.2 Preparation of composite strains

[0053] The spare liquid culture medium was divided into several 200mL Erlenmeyer flasks, 100mL in each bottle, and sterilized by high-pressure steam at 121°C for 15min. Finally, in the ultra-clean workbench, use an inoculation loop to pick some colonies from the slant of the test tubes of each component, inoculate them into each bottle of culture medium, and place them in a constant temperature shaker at 32°C and 150r / min for 24 hours. Lactobacillus and Lactobacillus plantarum static culture. Take 10L of spare liquid medium, inoculate Saccharomyces cerevisiae, Hansenula, Rhodopseudomonas palustris,...

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Abstract

The invention provides a microbial preparation for purifying sewage and deodorizing garbage. The microbial preparation is a liquid culture which comprises the following microorganisms: saccharomyces cerevisiae, hansenula polymorpha, rhodopseudomonas palustris, bacillus licheniformis, bacillus subtilis, bacillus megaterium, streptomyces microflavus, lactobacillus acidophilus, lactobacillus plantarum and streptococcus thermophilus. A liquid culturing medium comprises molasses, sodium chloride and urea. The number of viable bacteria in the microbial preparation is more than 1*1010cfu / ml. The microbial preparation can be added into the sewage or sprayed into a garbage transfer station, a garbage landfill and a farm to improve the environment significantly.

Description

technical field [0001] The invention belongs to the technical field of preparation of micro-ecological preparations, and in particular relates to a micro-ecological preparation for sewage purification and garbage deodorization and a preparation method thereof. Background technique [0002] Microecological preparations are widely used in agriculture, environmental protection, medical treatment and other fields. At present, they are used in the largest amount in agriculture, but not much in the field of environmental protection. Although some existing microecological preparations for water treatment have achieved some treatment effects, they generally have problems such as long activation time, short duration of action, and single microbial species, which lead to poor treatment effects. Contents of the invention [0003] In view of the shortage of existing micro-ecological preparations in the field of environmental protection, our R&D team was inspired by the concept of "eff...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/18C12N1/16C02F3/34A61L9/01C12R1/865C12R1/78C12R1/10C12R1/125C12R1/11C12R1/23C12R1/25C12R1/46C12R1/465C12R1/01A61L101/52
CPCC12N1/20A61L9/01C02F3/34C02F3/347C02F2101/16C12N1/16C12N1/18
Inventor 刘宫介张显忠葛文
Owner 上海寄绿生物环保科技有限公司
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