Microbial preparation for purifying sewage and deodorizing garbage and preparation method thereof

A technology for micro-ecological preparations and sewage purification, which is applied in microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., and can solve the problems of short duration of action, long activation time, and poor treatment effect. , to achieve the effect of environmental improvement

Inactive Publication Date: 2015-03-04
上海寄绿生物环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some existing micro-ecological preparations for water treatment have achieved some treatment effects, they generally have problems suc

Method used

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  • Microbial preparation for purifying sewage and deodorizing garbage and preparation method thereof
  • Microbial preparation for purifying sewage and deodorizing garbage and preparation method thereof
  • Microbial preparation for purifying sewage and deodorizing garbage and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0022] The preparation method of the composite strain is divided into two steps, one-stage and two-stage.

[0023] The above-mentioned "first level" is the pure culture of each component. Pick the colonies of each microorganism, respectively inoculate them into 100-200mL liquid culture medium, place them in a constant temperature shaker at 30-35℃, 100-200r / min for 16-24h; but Lactobacillus acidophilus and Lactobacillus plantarum do not shake , Stand still and cultivate. The inoculation amount of each microorganism is basically the same.

[0024] The above-mentioned "secondary" is mixed culture, the specific method is: prepare 5-10L liquid medium, inoculate the culture of Saccharomyces cerevisiae, Hansenula yeast, Rhodopseudomonas palustris, and Streptomyces tenuifolia after the first-level culture, 30~ 32 ℃ intermittent stirring (60~120r / min mechanical stirring, once every 3~5h, 1~2min each time) for 12~24h; then inoculate Bacillus licheniformis, Bacillus subtilis, Bacillus megat...

Example Embodiment

[0036] Example 1

[0037] 1.1 Preparation of liquid culture medium

[0038] Take 8% molasses, 2% salt, 1% urea, and 2% compound growth factor according to the mass ratio, and add 5 tons of medium with dechlorinated tap water. Among them, 2 tons are put into 3 tons of seed tanks, 2.5 tons are put into 10 tons of fermentation tanks, and the remaining 0.5 tons are reserved.

[0039] 1.2 Preparation of composite strains

[0040] Dispense the spare liquid culture medium into several 200 mL Erlenmeyer flasks, each 100 mL, and autoclave at 121°C for 15 min. Afterwards, in the ultra-clean workbench, use an inoculating loop to pick some colonies from the test tube slope of each component, inoculate them into each bottle of culture medium, and place them in a constant temperature shaker at 32°C, 150r / min for 24h, but they are fond of Lactobacillus acidi and Lactobacillus plantarum are cultivated in a static state.

[0041] Take 10L of spare liquid medium, inoculate Saccharomyces cerevisiae, Ha...

Example Embodiment

[0049] Example 2

[0050] 2.1 Preparation of liquid culture medium

[0051] Take 8% molasses, 2% salt, 1% urea, and 2% compound growth factor according to the mass ratio, and add 10 tons of medium with dechlorinated tap water. Among them, 4 tons are put into seed tanks, 5 tons are put into 20 tons fermentation tanks, and the remaining 1 ton is reserved.

[0052] 2.2 Preparation of composite strains

[0053] Dispense the spare liquid culture medium into several 200 mL Erlenmeyer flasks, each 100 mL, and autoclave at 121°C for 15 min. Then in the ultra-clean workbench, use an inoculating loop to pick some colonies from the test tube seed slope of each component, inoculate them into each bottle of culture medium, and place it in a constant temperature shaker at 32°C, 150r / min for 24h, acidophilic Lactobacillus and Lactobacillus plantarum are cultivated in a static state. Take 10L of spare liquid medium, inoculate Saccharomyces cerevisiae, Hansenula, Rhodopseudomonas palustris, Strepto...

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Abstract

The invention provides a microbial preparation for purifying sewage and deodorizing garbage. The microbial preparation is a liquid culture which comprises the following microorganisms: saccharomyces cerevisiae, hansenula polymorpha, rhodopseudomonas palustris, bacillus licheniformis, bacillus subtilis, bacillus megaterium, streptomyces microflavus, lactobacillus acidophilus, lactobacillus plantarum and streptococcus thermophilus. A liquid culturing medium comprises molasses, sodium chloride and urea. The number of viable bacteria in the microbial preparation is more than 1*1010cfu/ml. The microbial preparation can be added into the sewage or sprayed into a garbage transfer station, a garbage landfill and a farm to improve the environment significantly.

Description

technical field [0001] The invention belongs to the technical field of preparation of micro-ecological preparations, and in particular relates to a micro-ecological preparation for sewage purification and garbage deodorization and a preparation method thereof. Background technique [0002] Microecological preparations are widely used in agriculture, environmental protection, medical treatment and other fields. At present, they are used in the largest amount in agriculture, but not much in the field of environmental protection. Although some existing microecological preparations for water treatment have achieved some treatment effects, they generally have problems such as long activation time, short duration of action, and single microbial species, which lead to poor treatment effects. Contents of the invention [0003] In view of the shortage of existing micro-ecological preparations in the field of environmental protection, our R&D team was inspired by the concept of "eff...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/18C12N1/16C02F3/34A61L9/01C12R1/865C12R1/78C12R1/10C12R1/125C12R1/11C12R1/23C12R1/25C12R1/46C12R1/465C12R1/01A61L101/52
CPCC12N1/20A61L9/01C02F3/34C02F3/347C02F2101/16C12N1/16C12N1/18
Inventor 刘宫介张显忠葛文
Owner 上海寄绿生物环保科技有限公司
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