Method for determining prochloraz or metabolite of prochloraz in plant-derived food

Abstract

The invention provides a method for determining prochloraz or metabolite of prochloraz in plant-derived food. The method comprises the following steps: a, selecting the plant-derived food, adding organic solvent and sodium chloride into the plant-derived food, uniformly mixing, centrifuging the mixture, standing for layering the mixture, and taking the supernatant; b, adding anhydrous sodium sulfate into the supernatant acquired in the step a, uniformly mixing the anhydrous sodium sulfate with the supernatant, standing the mixture, and taking the supernatant; c, concentrating the supernatant acquired in the step b, making the volume constant, adding anhydrous sodium sulfate, N-propylethylenediamine and octadecyl silane bonded silica gel into the supernatant, uniformly mixing the mass, centrifuging the mixture, and taking the supernatant as a sample to be detected; and d, detecting the sample by adopting GC-MS / MS or GC-MS (Gas Chromatography-Mass Spectrometer). According to the method, a specific detection condition is adopted, linear response is good, and the residue of prochloraz or metabolite of the prochloraz can be accurately, rapidly and conveniently determined; meanwhile, the method has advantages of simplicity, rapidness, good selectivity, high sensitivity and good matrix interference resistance.

Description

technical field [0001] The invention relates to a method for determining prochloraz or its metabolites in plant-derived foods. Background technique [0002] Prochloraz is a broad-spectrum imidazole fungicide, which can prevent and control powdery mildew, anthracnose and other diseases. Preservation and preservation during post-storage and transportation, and prevention and treatment of diseases during storage. The final metabolite of prochloraz is 2,4,6-trichlorophenol, which has high toxicity and poor biodegradability, which is likely to cause serious environmental pollution and is suspected to be a strong carcinogen. The United Nations Food and Agriculture Organization / World Health Organization (FAO / WHO) Codex Alimentarius Commission (CCPR) stipulates that the maximum residue limit of prochloraz on crops includes the sum of the residues of prochloraz and its metabolites. Therefore, it is necessary to detect and control the residues of prochloraz and its metabolites in fr...

AI Technical Summary

Problems solved by technology

High-performance liquid chromatography (HPLC) can only detect the residual amount of prochloraz, but cannot detect the residual amount of its metabolite 2,4,6-trichlorophenol, and its sensitivity is lower than that of gas chromatography. For example, Yuan Xiaoya et al. (Determination of prochloraz 2,4,6-trichlorophenol residues in bananas by solid phase extraction-high performance liquid chromatography-tandem mass spectrometry. Yuan Xiaoya, Pan Yonggui, Wang Meiling, Dai Hua. Physical and Chemical Testing-Chemical Volume, 2014 No. 50 Volume) Determination of banana samples by solid phase extraction-high performance liquid chromatography-tandem mass spectrometry, only the residual amount of prochloraz was detected, but 2,4,6-trichlorophenol was not detected

Zhang Shirui et al. (Determination of prochloramine residues in two tropical fruits by GC. Zhang Shirui, Yuan Hongqiu, Yin Guihao. Modern Pesticides, August 2010, Volume 9, Issue 4) used GC to detect prochloramine in fruits residues, 2,4,6-trichlorophenol was not detected at the same time, and its sample pretreatment steps were complicated

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