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A kind of synthesis method of synthetic marijuana antigen and the application of synthetic marijuana antigen

A technology for synthesizing hemp and synthetic methods, which is applied in the field of immunoassays, can solve the problems of large space occupation, high price, unfavorable industrial production, etc., and achieve the effects of easy portability, long-term storage, and stability

Active Publication Date: 2018-09-18
ASSURE TECH HANGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The first three detection methods are precise, expensive, take up a lot of space, and take a long time, which is not conducive to the on-site use of anti-drug law enforcement departments
The ELISA method has a large throughput, but it is not convenient to carry around
[0004] In addition, the existing methods for synthesizing cannabis antigens use Grignard reagents and sodium hydride. These reagents are expensive, require harsh conditions, and have relatively large safety hazards, which are not conducive to industrial production.
The cross-linking arm of the existing detection antigen should be the same as the cross-linking arm of the immune antigen. In the process of antibody production, the cross-linking arm is likely to belong to the antibody recognition region

Method used

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  • A kind of synthesis method of synthetic marijuana antigen and the application of synthetic marijuana antigen
  • A kind of synthesis method of synthetic marijuana antigen and the application of synthetic marijuana antigen
  • A kind of synthesis method of synthetic marijuana antigen and the application of synthetic marijuana antigen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation of 1.3-(1-naphthoyl) indole: under the protection of nitrogen, in the round bottom flask, the naphthoyl chloride of 754.8mg is dissolved in 16ml of anhydrous dichloromethane, then add the anhydrous aluminum chloride of 717mg , stirred for 20min, the solution was yellow-green. Dissolve 351.45mg of indole in 6ml of anhydrous dichloromethane, then drop it into a round bottom flask, and continue stirring for 3h. The progress of the reaction was detected by TLC, and the developer petroleum ether: ethyl acetate = 4:1. After the reaction, the solution turns yellow; put the reaction system in an ice-water bath, then slowly add 45ml of ice water, transfer to a separatory funnel to separate the organic phase; extract with 2*45ml of dichloromethane, combine the organic phases, and wash with saturated saline , evaporated to dryness under reduced pressure, and obtained 606mg of 3-(1-naphthoyl)indole by column chromatography, yield 74.54%. The structure of the obtai...

Embodiment 2

[0052] The difference with Example 2 is that: during the preparation of 2-(3-(1-naphthoyl)indole-1-)ethyl acetate, ethyl bromoacetate is replaced with propyl bromoacetate.

Embodiment 3

[0054] A synthetic method for synthesizing cannabis antigens, comprising the following steps,

[0055] A. Take 90mg of (S)-2-(3-(1-naphthoyl)indole-1-)-N-(2-oxytetrahydrothiophene-3-)acetamide prepared in Example 1 and dissolve in 3mL methanol, then add 0.9mL 1N NaOH aqueous solution, after stirring at room temperature for 8min, add 0.9mL 1M hydrochloric acid to obtain (S)-2-(2-(3-(1-naphthylmethyl)indole-1- )acetamide)-4-mercaptobutanoic acid;

[0056] The structure of (S)-2-(2-(3-(1-naphthylmethyl)indole-1-)acetamide)-4-mercaptobutanoic acid is shown in formula (Ⅵ):

[0057]

[0058] B. SPDP is dissolved in DMF, obtains SPDP-DMF solution, and the concentration that makes SPDP in DMF is 28mg / mL;

[0059] Add 0.18mL SPDP-DMF solution dropwise into 1mL BSA solution, react at room temperature for 1h, remove unreacted SPDP, and obtain SPDP-BSA protein solution;

[0060] C. Take 42mL of the SPDP‐BSA protein solution prepared in step B, and drop into (S)‐2‐(2‐(3‐(1‐naphthylme...

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Abstract

The invention relates to the technical field of immunodetection and discloses a synthetic method for synthesizing a cannabis sativa antigen. The synthesized cannabis sativa antigen is obtained by coupling JWH series substances with macromolecular proteins through disulfide bonds. The invention further discloses application of the synthesized cannabis sativa antigen synthesized by the method to a colloidal gold detection card. According to the method, the synthesized JWH analogues are coupled with the macromolecular proteins through the disulfide bonds after being sulfhydrylated, and the chemical bonds of the JWH analogues are relatively stable, so that the antigen can be stored for a long term and the product stability can be well guaranteed; the cross-linking arms of the detection antigen are different from those of an immunizing antigen, so that nonspecific binding in an immunodetection process is reduced; in addition, the detection antigen with different cross-linking arms is guaranteed to be used for quick immunodetection; a Grignard reagent and sodium hydride are not used, so that the technical problems of severe operating conditions and a certain potential safety hazard during use of the Grignard reagent and sodium hydride are solved.

Description

technical field [0001] The invention relates to the technical field of immune detection, relates to a method for synthesizing a cannabis antigen, and in particular to the application of the synthetic cannabis antigen synthesized by the method. Background technique [0002] Synthetic marijuana (synthetic cannabis) is a new type of spice drug. It is a low-cost chemical synthetic drug that mixes different spices and herbs with different chemical substances to make different flavors. Its trade names include K2, Spice, Genie, Zohai, and Psychedelic Sage. Because this new type of spice does not contain natural cannabis active ingredients, in the early years of the 21st century, countries around the world basically did not include it in the scope of drug inspections, so it became popular in Europe and the United States and spread to Asia. However, studies in recent years have shown that the main psychoactive ingredients in synthetic cannabis JWH-018, JWH-073, JWH-250 and CP-47,497...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/765G01N33/558
CPCC07K1/1077C07K14/765C07K19/00G01N33/558
Inventor 凌世生
Owner ASSURE TECH HANGZHOU