Preparation method of chlorogenic acid uniformly-imprinted microspheres

Abstract

The invention discloses a preparation method of chlorogenic acid uniformly-imprinted microspheres, which comprises the following steps: dissolving chlorogenic acid and functional monomer MMA (methacrylic acid) in a mole ratio of 1:3-1:5 in an acetonitrile-toluene-DMSO (dimethyl sulfoxide) mixed solution, oscillating at room temperature for 3-7 hours, adding a crosslinking agent DVB (divinylbenzene) and a right amount of initiator AIBN (2,2'-azobisisobutyronitrile), transferring the mixture into a glass ampoul, introducing nitrogen for 5-15 minutes, sealing the ampoul, carrying out rotary polymerization at 60 DEG C for 24 hours, precipitating with methanol, precipitating with tetrahydrofuran, and filtering. The uniformly-imprinted microspheres with the diameter of about 2.5 mu m are prepared by using chlorogenic acid as the template molecule by a precipitation polymerization process. The polymer yield is 35-40%; and the molecularly imprinted polymer has uniform particle size and proper size, can be conveniently used as a liquid chromatography separation material and for preparation, separation and purification, and has the advantages of favorable separation efficiency, high extraction yield and wide application prospects.

Description

technical field [0001] The invention relates to a preparation method of chlorogenic acid uniform imprinted microspheres. Background technique [0002] The development of new high-selectivity separation materials is an important step to achieve high-efficiency separation. Molecularly imprinted polymer is a high molecular polymer that completely matches the target molecule in terms of space and binding sites after polymerizing target molecules, functional monomers, and cross-linking agents in a solution and removing the target molecules. This imprinted polymer can specifically recognize target molecules when extracting complex samples, so it is a separation medium with important application prospects. [0003] At present, although there are many studies on the preparation of molecularly imprinted polymers with polyphenolic compounds such as chlorogenic acid as templates, it is difficult to obtain microspheres with uniform particle size and excellent performance, which have th...

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Problems solved by technology

[0003] At present, although there are many studies on the preparation of molecularly imprinted polymers with polyphenolic compounds such as chlorogenic acid as templates, it is difficult to obtain microspheres with uniform particle size and excellent performance, and have the basic conditions for industrial application.

Molecularly imprinted microspheres with controllable particle size and regular shape can be prepared by dispersion polymerization, but the perfluoropolymer dispersant used is expensive and economical; although multi-step swelling method can be prepared in polar solvent Imprinted microspheres, but the operation process is cumbersome

[0004] In the past, we studied the preparation of caffeic acid-imprinted polymers by in-situ polymerization in empty chromatographic columns, and discussed their chromatographic identification and solid-phase extraction performance. Precipitation polymerization, but without success