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A kind of preparation method of immobilized enzyme

A technology of immobilizing enzymes and methyl acrylate, applied in directions such as being immobilized on/in an organic carrier, can solve the problems of difficulty in large-scale production and application, cumbersome processes, etc., to reduce non-specific interactions and simplify the process. , the effect of reducing costs

Active Publication Date: 2017-10-10
北京首医临床医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the above-mentioned technologies have disadvantages in improving carrier biocompatibility and immobilized enzymes, such as cumbersome processes, difficulty in large-scale production and application, etc.

Method used

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  • A kind of preparation method of immobilized enzyme
  • A kind of preparation method of immobilized enzyme
  • A kind of preparation method of immobilized enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A preparation method for immobilized enzyme, comprising the following steps:

[0030] (1), the mass ratio of keratin and poly(methyl acrylate-acrylic acid) is added into N,N-dimethylformamide solvent according to 1:19 to form a solution with a total mass fraction of 30%, and magnetically stirred at room temperature until Completely dissolve to obtain a uniform spinning solution, inject it into the electrospinning device, and electrospin under the conditions of a voltage of 18kV, a spinning flow rate of 0.2ml / h, and a receiving distance of 20cm to obtain a diameter of 600nm. Poly(methyl acrylate-acrylic acid) composite nanofiber membrane, the composite nanofiber membrane was dried in a vacuum oven at 25°C for 5 hours to obtain a composite nanofiber membrane of keratin and poly(methyl acrylate-acrylic acid), Observe the morphology and structure of composite nanofibers, such as figure 1 shown.

[0031] Wherein, the mass ratio of methyl acrylate to acrylic acid of the pol...

Embodiment 2

[0035] A preparation method for immobilized enzyme, comprising the following steps:

[0036](1), the mass ratio of keratin and poly(methyl acrylate-acrylic acid) is added into N,N-dimethylformamide solvent according to 1:4 to form a solution with a total mass fraction of 26%, and magnetically stir at room temperature until Completely dissolve to obtain a uniform spinning solution, inject it into the electrospinning device, and electrospin under the conditions of a voltage of 16kV, a spinning flow rate of 0.6ml / h, and a receiving distance of 15cm to obtain a diameter of 300nm. The poly(methyl acrylate-acrylic acid) composite nanofiber membrane is dried in a vacuum oven at 40° C. for 10 hours to obtain a composite nanofiber membrane of keratin and poly(methyl acrylate-acrylic acid).

[0037] The mass ratio of methyl acrylate to acrylic acid of the poly(methyl acrylate-acrylic acid) is 9:1.

[0038] (2) Weigh 5 mg of keratin and poly(methyl acrylate-acrylic acid) composite nanof...

Embodiment 3

[0043] A preparation method for immobilized enzyme, comprising the following steps:

[0044] (1), the mass ratio of keratin and poly(methyl acrylate-acrylic acid) was added into N,N-dimethylformamide solvent to form a 28% solution at a mass ratio of 1:9, and magnetically stirred at room temperature until it was completely dissolved. Obtain uniform spinning liquid, inject in the electrospinning device, be 18kV at the voltage, the spinning flow rate is 0.6ml / h, under the condition that receiving distance is 20cm, obtain the 400nm diameter keratin and poly(acrylic acid) methyl ester-acrylic acid) composite nanofiber membrane, the composite nanofiber membrane was dried in a vacuum oven at 25°C for 5 hours to obtain a composite nanofiber membrane of keratin and poly(methyl acrylate-acrylic acid). The mass ratio of methyl acrylate to acrylic acid of the poly(methyl acrylate-acrylic acid) is 19:1.

[0045] (2) Weigh 5 mg of keratin and poly(methyl acrylate-acrylic acid) composite na...

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Abstract

The invention provides a method for preparing immobilized enzymes. The invention uses a composite nanofiber membrane of keratin and poly(methyl acrylate-acrylic acid) as an immobilized enzyme carrier, and the prepared nanofiber membrane has high specific surface area and high porosity High efficiency, good biocompatibility, provide a bio-friendly microenvironment for immobilized enzymes, and effectively improve the catalytic activity and stability of immobilized enzymes. Compared with the prior art, the method provided by the invention has simple process, and the prepared immobilized enzyme has high enzyme loading capacity, good catalytic stability and good reusability.

Description

technical field [0001] The invention relates to the field of preparation of nanofiber membranes, in particular to a method for preparing immobilized enzymes. Background technique [0002] Enzyme is an important biocatalyst with the characteristics of high catalytic efficiency, strong specificity and mild reaction conditions. It is widely used in food, brewing, medicine and other fields. Due to the special properties of enzymes, their advanced structures are very sensitive to the environment, such as temperature, pH, organic solvents, heavy metal ions and other factors, and cannot be recovered after the reaction, which limits the industrial application of enzymes. [0003] The immobilized enzyme technology can effectively improve the stability and catalytic activity of enzymes under extreme conditions, and at the same time, it is easy to recycle and reuse. At present, there are many materials and methods for preparing immobilized enzymes. Among them, nanomaterials immobiliz...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/08
Inventor 李长龙周磊刘新华常桑唐文彬
Owner 北京首医临床医学科技有限公司
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