Stirred bioreactor convertible in flow direction and for artificial liver

Abstract

The invention discloses a stirred bioreactor convertible in flow direction and for an artificial liver. The stirred bioreactor convertible in flow direction and for the artificial liver comprises a bioreactor with a stirring paddle, wherein microcapsules of hepatic cells are loaded and embedded in the bioreactor. Due to the fact that the specific gravity of the microcapsules is approximate to the specific gravity of water, the microcapsules can easily enter a suspension state under stirring function, and accordingly the microcapsules can be fully mixed with liquid under a continuous perfusion operating condition high in flow speed, and simultaneously solid-liquid mass transfer is promoted, and furthermore sufficient nutrient substance supply and efficient active substance release of the hepatic cells in the microcapsules are guaranteed. Additionally, for avoiding the circumstance that the microcapsules or fragments block a filter screen, which possibly occurs in long time single pass perfusion, an electromagnetic valve and a peristaltic pump are respectively arranged at an inlet end and an outlet end of the bioreactor, conversion of flow directions can be achieved, backlash effects are achieved, a pipeline is prevented from jamming, and solid and liquid mixing in the bioreactor is kept even. The stirred bioreactor convertible in flow direction and for the artificial liver can achieve good solid and liquid mixing and substance exchange under the conditions that a solid-to-liquid ratio is large, the perfusion speed is high and the cell density is large, effectively avoids a perfusion dead space and invalid perfusion, and develops cure effects of the artificial liver to the utmost.

Description

technical field [0001] The invention relates to a medical device, in particular to a bioartificial liver or a hybrid artificial liver support system core device for the clinical treatment of acute liver failure: a stirring bioreactor with changeable flow direction. Background technique [0002] Acute liver failure is a common critical disease with a mortality rate as high as 65-95%. At present, artificial liver therapy has become an important means for the treatment of acute liver failure. The artificial liver support system is an extracorporeal mechanical or physical and chemical device that temporarily assists or completely replaces the function of the failed liver, removes various harmful substances, and compensates for the metabolic function of the liver. The biological artificial liver loaded with highly active liver cells can theoretically perform all the functions of liver synthesis and transformation, which is the trend and trend of the future development of artific...

AI Technical Summary

Problems solved by technology

The microcapsule-fixed bed reactor has a high cell loading density, and can reach a high operating flow rate, which can meet the oxygen supply of the cells and achieve good material exchange, but the microcapsules are pressure sensitive, and the high column produced by high flow rate perfusion High pressure can easily lead to microcapsule deformation and block the bed, while high shear force and continuous column pressure can easily cause microcapsule breakage

The main form of the microcapsule-suspension reactor is a fluidized bed reactor, which has the advantages of uniform mass transfer, low shear force, low column pressure, and is not easy to form inherent channels and perfusion dead spaces. Close, easy to be carried out by liquid during fluidization, the operating flow rate that can be achieved is low, which limits material exchange and cannot meet the oxygen demand of liver cells

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