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Preparation method of liquid preparation of bacillus pumilus and bdellovibrio

A technology of bacillus pumilus and liquid preparations, which is applied in the field of feed additives, can solve the problems that the preparation methods of liquid preparations of bacillus pumilus and bdellovibrio have not been reported, and achieve the reduction of vibriosis of prawns, the reduction of breeding costs, and the inhibition of growth Effect

Inactive Publication Date: 2015-03-25
SUZHOU IRIVET BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] But about the preparation method of the liquid preparation of Bacillus pumilus and Bdellovibrio of the present invention, there is no report yet

Method used

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  • Preparation method of liquid preparation of bacillus pumilus and bdellovibrio
  • Preparation method of liquid preparation of bacillus pumilus and bdellovibrio
  • Preparation method of liquid preparation of bacillus pumilus and bdellovibrio

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1 liquid preparation

[0022] The liquid preparation of the present invention can be obtained by separately carrying out liquid fermentation culture of Bacillus pumilus and Bdellovibrio, and then mixing the concentrates of the two to obtain the liquid preparation of the present invention. The liquid preparation prepared in this embodiment is used for the test of embodiment 3, embodiment 4, embodiment 5. The specific preparation method of the present embodiment is as follows:

[0023] The bacillus pumilus bacterial strain is cultivated in the order of slant, liquid seed, and liquid fermentation, and the slant and seed medium are conventional nutrient agar medium or broth medium; fermentation medium formula (W / V): bean cake powder 3% , 1.5% starch, 1% corn steep liquor, 0.5% glucose, (NH 4 ) 2 SO 4 0.1%, NH 4 Cl0.1%, KH 2 PO 4 0.05%, NaH 2 PO 4 0.5%, MgSO 4 0.05%, MnSO 4 0.05%, the fermentation culture condition is 28-37 ℃, ventilated culture 36-48h,...

Embodiment 2

[0026] Embodiment 2 powder preparation

[0027] The powdery preparation of the present invention can carry out solid-state fermentation culture respectively by Bacillus pumilus and Bdellovibrio respectively, then mix the powdery solid of the two; The two are mixed, and then dried to obtain a powder preparation. The powdered formulation prepared in this example was used in the tests of Example 4 and Example 5. The specific preparation method of the present embodiment is as follows:

[0028] Bacillus pumilus was inoculated in LB medium, cultured statically at 37°C for 12 hours, and the bacterial concentration was adjusted to 5×10 11 cfu / ml, according to the weight ratio Bacteria solution: Carrier is 1:1, add carrier corncob powder, dry at 60°C for 24 hours to make the number of viable bacteria ≥ 5×10 8 cfu / g Bacillus pumilus powder.

[0029] Inoculate 100ml of Bdellovibrio strains into 1 / 10NB medium, add 100L to a concentration of 1.0×10 6 cfu / ml Escherichia coli suspension, ...

Embodiment 3

[0031] Embodiment 3 suppresses vibrio spectrum analysis

[0032] The liquid preparation prepared in Example 1 was analyzed for inhibition of Vibrio by the flat filter paper diffusion method. The strength of the antibacterial ability was determined according to the size of the inhibition zone, and the results of the antibacterial spectrum analysis are shown in Table 1:

[0033] Table 1 Analysis of Vibrio Inhibitory Activity of Compositions

[0034]

[0035] Note: +++: Strong antibacterial activity; ++: Moderate antibacterial activity; +: Has antibacterial activity.

[0036] It can be seen from Table 1 that the liquid preparations of Bacillus pumilus and Bdellovibrio of the present invention have broad-spectrum inhibitory vibrio activity from the analyzed common pathogenic Vibrio.

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Abstract

The invention relates to a preparation method of a liquid preparation of bacillus pumilus and bdellovibrio. The preparation method comprises the following steps: respectively carrying out fermental cultivation on bacillus pumilus and bdellovibrio with a quorum sensing system inhibiting effect to prepare a concentrated liquid; and then mixing the bacillus pumilus and bdellovibrio. The preparation method provided by the invention has the advantages that the preparation method provided by the invention is simple in process and efficient and the prepared liquid preparation can be used for effectively eliminating diseases caused by vibrio in a broad-spectrum mode.

Description

technical field [0001] The invention relates to the technical field of feed additives, in particular to a preparation method of liquid preparations of Bacillus pumilus and Bdellovibrio. Background technique [0002] Vibrio belongs to the genus Vibrio in the Vibrio family. It is a kind of short rod-shaped bacteria with Gram-negative, polar flagella, motility and no spores. Some of them have a certain curvature, not soft, and some are curved Not obvious. Vibrio is one of the dominant groups of marine bacteria. It is widely distributed in water bodies and sediments in various estuaries, inner bays, coasts, aquaculture areas and open seas around the world. Various marine organisms often exist in high density on the surface or in their bodies. Marine Vibrio occupies an important ecological position in the marine environment, has a variety of physiological and ecological functions, plays an important role in the marine microfood cycle, and plays a very important role in the cycle...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/18A23K1/16C12N1/20C12R1/01C12R1/07
Inventor 宋增福许维素
Owner SUZHOU IRIVET BIOTECH
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