Rhodamine B-based fluorescence sensor and preparation

A technology of fluorescent sensor and aminocaproic acid, which is applied in the field of fluorescent sensor and its preparation, can solve the problems of expensive detection and insufficient fast detection, and achieve the effects of good selectivity, high sensitivity, and resource saving

Inactive Publication Date: 2015-03-25
NANJING UNIV OF SCI & TECH
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  • Abstract
  • Description
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Problems solved by technology

However, these methods have many disadvantages, such as sample pretreatment, detection is not fast enough, detection is expensive, etc.

Method used

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  • Rhodamine B-based fluorescence sensor and preparation
  • Rhodamine B-based fluorescence sensor and preparation
  • Rhodamine B-based fluorescence sensor and preparation

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Embodiment Construction

[0022] The synthetic route of the present invention is as follows:

[0023]

[0024] 1. Synthesis of target compounds

[0025] 1. Add 100 mg of 3-(3',6'-bis(diethylamino)-3-oxaspiro[isoindoline-1,-1,9'-xanthene]-2-yl)propane (see the article Design and synthesis of a novel Rhodamine B[2]rotaxane) was dissolved in 5mL of methanol, then 79mg of 6-aminocaproic acid was added, and stirred at room temperature for 2h;

[0026] 2. Weigh 85mg of sodium triacetylborohydride, dissolve it in 1ml of tetrahydrofuran, add it to the above reaction solution with a pipette gun, continue to stir at room temperature, use TLC to detect the reaction progress, and the reaction is complete in 3 hours;

[0027] 3. Add 1 mL of saturated sodium bicarbonate solution dropwise to the above reaction solution, and continue to stir for 10 minutes. The saturated sodium bicarbonate is to quench the sodium triacetylborohydride added thereto;

[0028] 4. Remove the solvent with a rotary evaporator, and sepa...

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Abstract

The invention discloses a rhodamine B-based fluorescence sensor and preparation. The preparation comprises the following steps: dissolving 3-(3',6'-bi(diethyl amino)-3-oxaspiro[isoindoline-1,-1,9'-xanthene]-2-yl) propanal (A) in methyl alcohol, adding 6-aminocaproic acid (B), and stirring at room temperature; dissolving sodium triacetoxyborohydride in tetrahydrofuran, adding to a reaction liquid in the step 1, and further carrying out stirring reaction at room temperature; dropwise adding a saturated sodium bicarbonate solution to the reaction liquid, and further stirring; and removing a solvent by virtue of a rotary evaporator, and separating by using a thin layer chromatography, so as to obtain a target product. The rhodamine B-based fluorescence sensor is relatively simple in synthesis step; resources are saved; and nearly all the reported fluorescence probes can only be applied to an organic solvent or an organic water solution, thus the application of the fluorescence sensor in the fields of actual water sample analysis and life is limited.

Description

technical field [0001] The invention relates to a rhodamine B-based fluorescent sensor and its preparation, belonging to the field of biological fluorescent sensor preparation. Background technique [0002] Tin is one of the essential trace elements in the human body. It has an important impact on various physiological activities of the human body and the maintenance of human health. Its main physiological functions are anti-tumor and promotion of protein and nucleic acid synthesis. Studies have found that the tin content in tumor tissue is lower than other normal tissues. The lack of tin in the human body will lead to abnormal metabolism of protein and nucleic acid, but if there is too much tin accumulated in the human body, adverse symptoms such as dizziness, diarrhea, and nausea may occur. Chromium is also an essential trace element in the human body. It is an important element for normal growth and development and regulation of blood sugar. Too low or too high levels o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D491/107C09K11/06G01N21/64
CPCC07D491/107C09K11/06C09K2211/1088G01N21/6428
Inventor 包晓峰陈晓燕曹乾升曹小伟
Owner NANJING UNIV OF SCI & TECH
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