Method for extracting cordycepin from cordyceps militaris rice medium under ultrahigh pressure

A culture medium, the technology of Cordyceps militaris, applied in the biological field, can solve the problems of unsuitable large-scale preparation of cordycepin and limited injection volume, etc., and achieve the effects of saving material consumption, high recovery rate and improving yield

Active Publication Date: 2015-03-25
武汉添年科技有限公司
View PDF8 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the limited amount of injection, it is not suitable for large-scale preparation of cordycepin

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting cordycepin from cordyceps militaris rice medium under ultrahigh pressure
  • Method for extracting cordycepin from cordyceps militaris rice medium under ultrahigh pressure
  • Method for extracting cordycepin from cordyceps militaris rice medium under ultrahigh pressure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Take 30.0 g of Cordyceps militaris culture medium powder that has been crushed and passed through a 20-mesh sieve in a sealable plastic container, add distilled water at a material-to-liquid ratio of 1:70, stir evenly to make it fully wet, and use a vacuum packaging machine to degas it. Make sure that there are no air bubbles in the container, then put it into the ultra-high pressure treatment device, add tap water as the loading medium into the treatment chamber, use the depth gauge to check the liquid level until the height is appropriate, remove the depth gauge, push the plunger to the pressurized position, and close the supercharger. The high-voltage main engine protection door is ready to start pressurization. Set the pressure to 400MPa, and hold the pressure for 15 minutes. After the treatment is completed and the pressure is relieved, take out the container, pour out the extract and centrifuge (4000r / min, 10min), and discard the residue to obtain an ultra-hig...

Embodiment 2

[0034] The difference from Example 1 is that the used medium powder of Cordyceps militaris was crushed through a 60-mesh sieve, and its content was finally measured to be 41.09%, and the ultra-high pressure extraction yield was 1558.556ug / g, and the crude cordycepin was extracted by ultra-high pressure after freeze-drying The mass is 1.311g, and the purity of the dried crude cordycepin is 94.02% after separation by countercurrent chromatography.

Embodiment 3

[0036] The difference from Example 1 is that the used Cordyceps militaris culture medium powder is crushed through a 60-mesh sieve, and the pressure is 600Mpa. Finally, the content of cordycepin in the ultra-high pressure extract is measured to be 40.57%, and the extraction yield is 1393.71ug / g. The quality of the crude cordycepin extracted by ultra-high pressure after drying was 1.142g, and the purity of the dried crude cordycepin was 91.82% after separation by countercurrent chromatography.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for extracting cordycepin from a cordyceps militaris rice medium under ultrahigh pressure, and belongs to the technical field of natural products. The technological process of the method comprises the following steps: performing slice on the cordyceps militaris rice medium, drying with hot air, crushing, extracting under ultrahigh pressure, performing centrifugation, removing impurities through a ceramic membrane, concentrating, freeze-drying, purifying cordycepin through a high-speed countercurrent chromatography, and detecting through a high performance liquid chromatography. According the method, the yield of the cordycepin from the cordyceps militaris rice medium is 1.2 times that of a common method, and the purity can reach more than 90%. The method adopts ultrahigh pressure to extract the cordycepin, so that advantages of high extraction rate, low impurity content, good stability and the like are realized; high temperature is prevented in the whole extraction process, so that active ingredients and structures of the cordycepin can be effectively retained. The cordycepin prepared by the method is safe and free from contamination, has a high food (drug) use value, and provides a certain market application prospect and economic benefits for comprehensive utilization of edible fungus cordyceps militaris by-products.

Description

technical field [0001] The invention specifically relates to a method for rapidly and efficiently extracting cordycepin from rice medium of Cordyceps militaris, belonging to the field of biological technology. Cordycepin in Cordyceps militaris medium was extracted by ultra-high pressure, and impurities were initially removed from the extract by ceramic membrane, and high-purity cordycepin was finally prepared by high-speed countercurrent chromatography. Background technique [0002] Cordyceps militaris, as a medicinal (edible) fungus, has a similar effect to Cordyceps sinensis, and has been recognized and widely accepted by the people of our country. With the rise of market demand, the technology of artificial cultivation of Cordyceps militaris continues to mature and is widely used in the market, and while achieving good market effects and economic benefits, the problem that arises is that the residual medium after harvesting fruiting bodies is discarded in The environment...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07H19/16C07H1/06
CPCC07H1/06C07H19/16
Inventor 程薇高虹陈丽冰吴光旭史德芳范秀芝郭鹏陈明利薛淑静杨德乔宇汪兰吴文锦李露
Owner 武汉添年科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap