A kind of hypoheme dipeptide and its preparation method and application
A technology of hypoheme and its application, which is applied in the field of hypoheme dipeptide and its preparation, can solve problems such as no public reports, and achieve good application prospects, small molecular weight, and simple operation
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0035] The solid-phase synthesis of embodiment 1 heme dipeptide
[0036] ①Resin swelling: weigh 5mmol Rink-NH 2 Resin, add 100ml dichloromethane to swell for 2h, filter with suction, wash resin 3 times with 100ml DMF (dimethylformamide);
[0037] ② Deprotection: add 200ml 20% piperidine / DMF solution, shake at room temperature for 30min, filter with suction, wash the resin 3 times with DMF, use a chromogenic reagent to detect the deprotection of amino groups, when the resin particles all turn blue, it means deprotection completely;
[0038] ③ Connected amino acid: Fmoc-α-Ala-OH, PyBOP (benzotriazol-1-yl-oxytripyrrolidinylphosphonium hexafluorophosphate), HOBT (1-hydroxybenzotriazole), NMM (N -Methylmorpholine) and Rink-NH 2 The resin is added into the reactor sequentially at a molar ratio of 2.5:2.5:2.5:2.5:1, 100ml of dimethylformamide is added to shake at room temperature for 1 hour, filtered with suction, the resin is washed 3 times with dimethylformamide, and detected wi...
Embodiment 2
[0044] Example 2 Comparison of the protective effects of vitamin C, hypoheme dipeptide, hypoheme dipeptide analogs and other hypoheme-short peptide compounds on improving the stability of capsanthin under light conditions
[0045] According to Table 1, take 25 μL of vitamin C / acetone solution, hypoheme dipeptide / acetone solution, hypoheme dipeptide analog / acetone solution and hypoheme-short peptide compound / acetone solution with a concentration of 50 μg / mL, and add 5.0 The capsanthin / acetone solution (mass ratio of 1:100) with a mL concentration of 50 μg / mL was mixed evenly, placed under light conditions for 5 days, the color value of each solution was measured, and the residual rate of the color value of each solution was calculated. See Table 1 for color valence residual rate.
[0046] Color value of capsanthin According to the national standard GB10783-2008 [14] Measured by UV spectrophotometry. Indicates the absorbance at 460nm of 0.5% of the tested sample in a 1cm cu...
Embodiment 3
[0051] Example 3 Comparison of the protective effects of vitamin C, hypoheme dipeptide, hypoheme dipeptide analogs and other hypoheme-short peptide compounds on improving the stability of capsanthin under high temperature conditions
[0052] According to Table 1, take 25 μL of vitamin C / acetone solution, hypoheme dipeptide / acetone solution, hypoheme dipeptide analog / acetone solution and hypoheme-short peptide compound / acetone solution with a concentration of 50 μg / mL, and add 5.0 The capsanthin / acetone solution (mass ratio: 1:100) with a mL concentration of 50 μg / mL was mixed evenly, and placed at 60°C for 5 days to measure the color value of each solution, and calculate the residual rate of the color value of each solution. See Table 2 for the color value residual rate of the solution
[0053] Table 2: Comparison of hypoheme dipeptide and vitamin C, hypoheme dipeptide analogues and other hypoheme-short peptide compounds on the protection of capsanthin
[0054] hypoh...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More