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Method for preparing and using low-heavy-metal-content chlorella cultivation raw material mother solution

A technology of low heavy metals and chlorella, applied in the direction of microorganism-based methods, biochemical equipment and methods, applications, etc., can solve the problems of excessive heavy metals in products, high cost, excessive heavy metals, etc., and achieve simple and easy operation and control Exposure chance, raw material simple effect

Inactive Publication Date: 2015-04-08
东莞市绿安奇生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, during the cultivation and production of commercial microalgae such as Chlorella, Spirulina, Salina and Haematococcus pluvialis, the problem of excessive heavy metals in the products is often encountered, which brings economic losses to algae cultivation enterprises. and may cause health hazards to product users
The problem of excessive heavy metals is the biggest unfavorable factor affecting the production of microalgae cultivation
[0003] In order to reduce the content of common heavy metals such as lead, arsenic, cadmium, and mercury in the production of microalgae, flocculants, chelating agents, etc. are generally used in the raw material mother liquor, but the effect of reducing heavy metals is limited, or the cost is high, and it is even possible Unsuitable for microalgae cultivation due to adverse effects of improper use on chlorella cultivation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Humic acid pretreatment: the specific operation method is to put 50 kg of humic acid into a 1t-grade plastic vat, add 500 kg of nitric acid with a concentration of 0.1% (m / v), stir well, and centrifuge with a disc centrifuge Separation, the rotation speed is 4500rpm; add 250 kg of clean water to the thick slurry material, stir and mix well, and then centrifuge again. This step is repeated twice; collect the supernatant (including fulvic acid) obtained from each step of centrifugation for the preparation of trace elements; finally centrifuge The thick slurry obtained is humic acid, sampled and dried, and the background content of each heavy metal is measured, and the detection method is in accordance with the corresponding national standard method. After testing, among the humic acid raw materials, the insoluble component (the main component is humic acid) is about 87%, and the soluble component (the main component is fulvic acid) is about 13%. Among the insoluble compone...

Embodiment 2

[0029] Configuration of raw material elements for cultivation: urea, ammonium bicarbonate, magnesium sulfate, sodium chloride, ferrous sulfate and other raw materials used in chlorella cultivation are dissolved in water respectively, and are configured to the concentration required for cultivation and 100 times the concentration of the working solution.

Embodiment 3

[0031] Potassium dihydrogen phosphate de-heavy metal mother liquor configuration: Agricultural fertilizer potassium dihydrogen phosphate usually contains a relatively high content of heavy metal arsenic, weigh 50 kg of agricultural fertilizer potassium dihydrogen phosphate (the arsenic content is 8.3 mg / kg), add 500 kg of water, add 20 kg (dry weight) of the insoluble component obtained in Example 1 was adjusted to pH 5 with nitric acid at a concentration of 0.1 mol / L, stirred by a water pump for more than 10 minutes, centrifuged with a disc centrifuge at a speed of 4500 rpm, and the thick slurry was In order to absorb the humic acid suspension of heavy metals, after sampling and drying, the heavy metal arsenic content in it was tested to be 18.3mg / kg; the clear liquid is the mother liquor of potassium dihydrogen phosphate for heavy metal removal, which can be used for chlorella cultivation after blending .

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Abstract

The invention provides a method for preparing and using a low-heavy-metal-content chlorella cultivation raw material mother solution. The method is characterized by comprising the following steps: pretreating humic acid used as a main material to obtain liquid fulvic acid and solid humic acid; mixing the liquid fulvic acid with a micro-element raw material to prepare a mother solution, and promoting dissolution of the microelement; mixing the solid humic acid with a high-heavy-metal-content raw material mother solution, and adsorbing heavy metals by using humic acid to reduce the heavy metal content of the raw material mother solution, thereby obtaining a solid part namely humic acid with the adsorbed heavy metals; and removing the heavy metals from humic acid with the adsorbed heavy metal by virtue of an acid cleaning method to realize regeneration. The microelement cultivation raw material mother solution containing humic acid and the constant cultivation raw material mother solution without the heavy metals, which are proportioned according to certain used amounts, can be used for cultivating various chlorella, and the method is low in cost, simple to operate, capable of effectively reducing the heavy metal content of chlorella cultivation raw materials, and capable of effectively promoting the growth of chlorella and effectively improving the product quality and yield of chlorella.

Description

technical field [0001] The invention relates to the technical field of chlorella biological cultivation, in particular to a method for reducing the heavy metal content in the chlorella cultivation raw material mother liquor and a method for using the cultivation raw material mother liquor. Background technique [0002] Microalgae have the biological characteristics of absorbing and enriching heavy metals. In the process of microalgae cultivation, the raw material mother liquor used for algae growth is due to the water used, various fertilizers, especially mineral fertilizers such as phosphorus and potassium fertilizers and table salt, and construction and cultivation. The cement used in the pool and the ambient air contain heavy metal ions of different types and concentrations, which makes the raw material mother liquor rich in heavy metal ions. Microalgae cells can absorb heavy metal ions in the mother liquor of the medium raw material and absorb them into the cells. There...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C12N1/12C12R1/89
CPCC05B1/02C05G3/00C12N1/12C12N1/125C12R2001/89C05B7/00C05C1/00C05C3/00C05C5/02C05C9/00C05D1/02C05D3/00C05D5/00C05D9/00C05D9/02
Inventor 俞建中
Owner 东莞市绿安奇生物工程有限公司
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