Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for extracting and preparing tanshinone compounds from dregs of radix salviae miltiorrhizae by virtue of saccharification pretreatment method

A technology for tanshinone and medicinal residues is applied in the field of tanshinone-based components, extraction and preparation of tanshinone-based components, which can solve the problems of inefficient and high-value utilization of danshinone medicinal residues, waste of resources, etc., so as to improve resource utilization efficiency, reduce environmental pollution, The effect of good eco-efficiency

Inactive Publication Date: 2015-04-08
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
View PDF2 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, most of the dregs of salvia miltiorrhiza are incinerated or landfilled, and a few are used as bio-organic fertilizers and animal feed additives for simple treatment and low-value utilization, resulting in a huge waste of resources
Therefore, the establishment of a method for preparing tanshinone components using Danshen dregs as raw material with a feasible process can solve the current technical problem that Danshen dregs cannot be utilized efficiently and with high value, and contribute to the improvement of the utilization efficiency of Danshen resources and the resource utilization of Danshen dregs. Utilization and industrialization have important application value and ecological benefits

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting and preparing tanshinone compounds from dregs of radix salviae miltiorrhizae by virtue of saccharification pretreatment method
  • Method for extracting and preparing tanshinone compounds from dregs of radix salviae miltiorrhizae by virtue of saccharification pretreatment method
  • Method for extracting and preparing tanshinone compounds from dregs of radix salviae miltiorrhizae by virtue of saccharification pretreatment method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Take 500g of salvia miltiorrhiza, mix it with acetic acid-sodium acetate buffer solution with a pH value of 4.8 according to the mass volume ratio of 1:1, then add cellulase solution, and place it in a constant temperature shaker at 50°C and 200r / min Carry out enzymatic hydrolysis for 24 hours;

[0039] (2) Add 500 ml of ethyl acetate to the enzymolysis solution and extract 3 times, combine the extracts, recover the solvent under reduced pressure, and obtain the concentrate;

[0040](3) Take the concentrate and dissolve it in an appropriate amount of ethanol solution, mix the sample, apply D101 macroporous adsorption resin, use 10%, 30%, 50%, 70%, 90% ethanol to elute, collect 70% ~ 90% ethanol to wash Deliquification, concentration under reduced pressure, spray drying to obtain the total tanshinone extract powder, the HPLC test results show that the total tanshinone purity is 92.3%, wherein the weight percentage of tanshinone IIA is 36.92%, and the weight percentag...

Embodiment 2

[0042] (1) Take 500g of salvia miltiorrhiza dregs, mix it with acetic acid-sodium acetate buffer solution with a pH value of 4.8 according to the mass volume ratio of 1:2, then add cellulase solution, and place it in a constant temperature shaker at 50°C and 200r / min. Enzymolysis for 24 hours;

[0043] (2) Add 8% Na to the enzymolysis solution 2 CO 3 The solution was mixed uniformly, extracted three times with 800 ml of ethyl acetate, combined the extracts, recovered the solvent under reduced pressure, and obtained the concentrate;

[0044] (3) Take the concentrate and dissolve it in an appropriate amount of ethanol solution, mix the sample, apply D101 macroporous adsorption resin, use 15%, 30%, 50%, 70%, 95% ethanol to elute, collect 70% ~ 95% ethanol to wash Deliquification, concentration under reduced pressure, spray drying to obtain the total tanshinone extract powder, the HPLC detection results show that the total tanshinone purity is 90.06%, wherein the tanshinone IIA ...

Embodiment 3

[0046] (1) Take 500 g of Danshen herb dregs, mix them with 10% HCl solution according to the mass volume ratio of 1:2, and put them in a water bath at 50°C for acidolysis for 24 hours;

[0047] (2) Take the hydrolyzed solution to adjust the pH value to neutral, add 500ml of ethyl acetate for solvent extraction 3 times, combine the extracts, recover the solvent under reduced pressure, and obtain the concentrate;

[0048] (3) Dissolve the concentrate in an appropriate amount of ethanol solution, mix the sample, apply AB-8 macroporous adsorption resin, use 10%, 30%, 50%, 70%, 95% ethanol to elute, and collect 70% to 95% The ethanol eluate is concentrated under reduced pressure and spray-dried to obtain the total tanshinone extract powder. The HPLC detection results show that the total tanshinone purity is 70%, wherein the tanshinone IIA weight percent is 22.52%, and the tanshinone I weight percent The content is 21.98%, the weight percentage of cryptotanshinone is 5.27%, and the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
purityaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for extracting and preparing tanshinone compounds from dregs of radix salviae miltiorrhizae by virtue of a saccharification pretreatment method. The method comprises the steps of firstly carrying out saccharification treatment on waste dregs of radix salviae miltiorrhizae to damage cell wall cellulose, hemicelluloses and lignocelluloses, extracting treatment liquid by virtue of an organic solvent, concentrating extraction liquid to a certain volume, carrying out ethanol elution on a macroporous resin column, collecting optimized eluate parts, concentrating, and carrying out spray drying, so as to prepare chemical components of tanshinone. According to the method, the extraction rate can reach 80%-100%, and the purity can reach 70%-95%; the content of tanshinone IIA can reach 36%, the content of tanshinone I can reach 32%, the content of cryptotanshinone can reach 8%, the content of dihydrotanshinone can reach 9%, and the content of active ingredients is high. Experiment results prove that the tanshinone compounds have well efficacies of promoting blood circulation and removing stasis and can be used for preparing medicines or functional health products capable of preventing and treating cardiovascular and cerebrovascular diseases.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine, and relates to a method for resource utilization of discarded salvia miltiorrhiza dregs, in particular to a method for extracting and preparing tanshinone components from discarded salvia miltiorrhiza dregs, and the preparation and use of the tanshinone components. Background technique [0002] Salvia miltiorrhiza Bge. is the dry root and rhizome of Salvia miltiorrhiza Bge. It was first recorded in "Shen Nong's Materia Medica" and listed as top grade. Said: "Salvia miltiorrhiza is bitter and slightly cold, governs evil spirits in trusted subordinates, bowel sounds faint like running water, accumulation of cold and heat, breaking symptoms and removing lumps, relieving restlessness and fullness, and benefiting qi." Clinically, it is mostly used to treat irregular menstruation, amenorrhea dysmenorrhea, accumulation of lumps in the abdomen, tingling pain in the chest and abdomen...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07J73/00A61K31/58A61P9/00A23L1/30A23L33/105
CPCA61K36/537A23V2002/00A61K31/58A61K2236/10A61K2236/19A61K2236/333A61K2236/51C07J73/00A23V2200/326A23V2250/21
Inventor 宿树兰段金廒赵步长唐志书王明耿钱大玮郭盛
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com