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Magnetic immobilized cellobiase nano mesoporous material and preparation method thereof

A technology of nano-mesoporous materials and cellobiase, which is applied in the directions of immobilized on or in inorganic carriers, fixed on/in organic carriers, etc., can solve the problems of recovery of immobilized enzymes, and the number of repeated uses is small , limit applications and other issues, to achieve the effect of improving storage stability and use temperature, reducing production costs and prolonging the storage period

Inactive Publication Date: 2015-04-08
TSINGHUA UNIV
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The immobilized enzyme using polymer gel material as the carrier has short service life, high cost, poor anti-toxicity, low activity and less repeated use times; the sol-gel process and template of immobilized enzyme are prepared by surfactant gelation method The removal process often requires harsh conditions (such as high temperature and high pressure, strong acid-base environment, and the use of a large amount of organic solvents), which have a great destructive effect on biologically active substances (such as: proteins and nucleic acids), thus extremely This greatly limits the application of the traditional surfactant method in the biological field, and there are also problems in the recovery of the immobilized enzyme prepared by this method

Method used

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  • Magnetic immobilized cellobiase nano mesoporous material and preparation method thereof
  • Magnetic immobilized cellobiase nano mesoporous material and preparation method thereof
  • Magnetic immobilized cellobiase nano mesoporous material and preparation method thereof

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preparation example Construction

[0032] According to preparation method of the present invention and above-mentioned principle picture , the method further enters the step of removing the template and drying step S1300: grind the gel, wash with water to remove the template and obtain the gel by suction filtration, embed the gel obtained by suction filtration into silica gel for drying for 3-7 days, and dry the dried The gel is ground through a 40-mesh sieve and packed into bags for later use.

[0033] The activity of the mesoporous material immobilized cellobiase product prepared according to the present invention can reach more than 80% of the activity of free cellobiase, and can be reused more than ten times without reducing the activity; The magnetic nanoparticles can be recycled and used, thereby greatly reducing the production cost of cellulosic ethanol; the use of mesoporous materials to immobilize cellobiase can improve the storage stability and use temperature of cellobiase, and prolong its storage p...

Embodiment 1

[0038] according to picture The preparation method of the magnetically immobilized cellobiase nano-mesoporous material of the present invention shown in 1 first enters the sol formation step S1100: take 32 grams of tetraethyl orthosilicate (TEOS), add 5 grams of 0.1M The dilute HCl solution, the mass ratio of hydrochloric acid solution to TEOS is 5:32, after vigorous stirring for 10 minutes, TEOS is hydrolyzed at room temperature with dilute acid as a catalyst to form a transparent sol, and the hydrolyzed transparent sol is cooled at 0°C for 2 hours Finally, use a high-vacuum system to extract the by-product ethanol produced in the hydrolysis process of the system. After the extraction for a period of time, keep the temperature of the sol water bath at 37°C for extraction until the mass content of ethanol in the sol is ≤5%.

[0039] Then enter the gel formation step S1200: add template solution, cellobiase buffer and magnetic nano-Fe in sequence to the sol under stirring 3 o...

Embodiment 2

[0042] according to picture The preparation method of the magnetically immobilized cellobiase nano-mesoporous material of the present invention shown in 1 first enters the sol formation step S1100: take 32 grams of tetraethyl orthosilicate (TEOS), add 13 grams of 0.3 M The mass ratio of hydrochloric acid solution to TEOS is 13:32. After stirring vigorously for 20 minutes, TEOS is hydrolyzed at room temperature with dilute acid as a catalyst to form a transparent sol, and the hydrolyzed transparent sol is cooled at 10°C for 4 hours. Finally, use a high-vacuum system to extract the by-product ethanol produced in the hydrolysis process of the system, and after a period of extraction, keep the temperature of the sol water bath at 50°C for extraction until the mass content of ethanol in the sol is ≤5%.

[0043] Then enter the gel formation step S1200: add template solution, cellobiase buffer and magnetic nano-Fe in sequence to the sol under stirring 3 o 4 Particles, adjust the s...

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Abstract

The invention discloses a preparation method of a magnetic immobilized cellobiase nano mesoporous material, which comprises the following steps: a sol forming step: dilute acid is taken as a catalyst for hydrolyzing tetraethoxysilane to form transparent sol, ethanol is dissociated from the sol until mass content is less than or equal to 5%; and a gel forming step: a template solution, a cellobiase buffer and magnetic nanoparticles Fe3O4 in sol under stirring, adjusting a system to neutrality by a dilute alkali solution, and placing to generate the gel. The invention also discloses the magnetic immobilized cellobiase nano mesoporous material prepared by the method. aperture of a nano medium material obtained by the method is about 2-7 nm, so that specific surface area is high; the nano medium material can be gathered in short time under magnetic field effect, so that recovery and usage are convenient; and the nano medium material has the advantages of high biological activity and reutilization.

Description

technical field [0001] The invention relates to a nano mesoporous material and a preparation method thereof, in particular to a magnetically immobilized cellobiase nano mesoporous material and a preparation method thereof. Background technique [0002] Affected by factors such as world oil supply, price, environmental protection and global climate change, the development of renewable clean energy-biofuels has attracted the attention of many countries, especially the United States, Brazil and China. Biofuel is mainly fuel ethanol, and biotechnology enzymatic method is one of the important steps to obtain fuel ethanol from cellulose, which is the largest amount of renewable carbon source material on the earth. However, the high degree of polymerization of cellulose makes it difficult to degrade. Compared with starch, the enzymatic hydrolysis rate of cellulose is more than 2 orders of magnitude lower, which makes the cost of cellulase too high, which seriously restricts the ind...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/04
Inventor 危岩李书润张翔
Owner TSINGHUA UNIV
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