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Method for extracting phosphatide and sea cucumber oil by utilizing sea cucumber blanching liquid

A technology of blanching liquid and sea cucumber, which is applied in the direction of edible phospholipid composition, food extraction, chemical instruments and methods, etc., can solve the problems of extracting phospholipid and sea cucumber oil, etc., achieve high nutritional and health value, and prevent senile dementia , The effect of low production cost

Active Publication Date: 2015-04-22
SHANDONG HOMEY AQUATIC DEV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there is no report of extracting phospholipids and sea cucumber oil from sea cucumber blanching liquid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A method for extracting phospholipids and sea cucumber oil from sea cucumber blanching liquid, through the following process steps:

[0031] (1) Selection and processing of raw materials

[0032] Select 4000L of sea cucumber blanching liquid as a raw material, concentrate it under reduced pressure through a vacuum concentration equipment, concentrate to a volume of 800L, and have a solid content of 10%, to obtain a concentrated solution;

[0033] (2), enzymatic hydrolysis

[0034] Heat the concentrated solution obtained in step (1) to 100°C for 30 minutes, fully dissolve, cool, and control the temperature to 55°C, then add a compound protease with a concentrated liquid quality of 1‰ to the concentrated solution, and perform enzymatic hydrolysis for 1 hour to obtain an enzymolyzed solution ; Among them, the composite protease is a composite protease produced and sold by Ningxia Hersbit Biotechnology Co., Ltd.;

[0035] (3), inactivate enzyme

[0036] Heat the enzymati...

Embodiment 2

[0048] A method for extracting phospholipids and sea cucumber oil from sea cucumber blanching liquid, through the following process steps:

[0049] (1) Selection and processing of raw materials

[0050] Select 4000L of sea cucumber blanching liquid as a raw material, send it into a vacuum concentration equipment for vacuum concentration, concentrate to a volume of 1000L, and a solid content of about 5%, to obtain a concentrated solution;

[0051] (2), enzymatic hydrolysis

[0052] Heat the concentrated solution obtained in step (1) at 120°C for 10 minutes, fully dissolve, cool the temperature to 60°C, then add a compound protease with a concentrated solution quality of 2‰ to the concentrated solution, enzymatically hydrolyze it for 1 hour, and then add a concentrated solution with a quality of 2‰ ‰ of flavored protease, hydrolyzed for 2.5h to obtain enzymatic hydrolyzate; wherein, the compound proteases are commercially available Novozymes compound proteases, and the flavored...

Embodiment 3

[0066] A method for extracting phospholipids and sea cucumber oil from sea cucumber blanching liquid, through the following process steps:

[0067] (1) Selection and processing of raw materials

[0068] Select 4000L of sea cucumber blanching liquid as a raw material, send it into a vacuum concentration equipment for vacuum concentration, concentrate to a volume of 500L, and a solid content of about 15%, to obtain a concentrated solution;

[0069] (2), enzymatic hydrolysis

[0070] Heat the concentrated solution obtained in step (1) at 90°C for 30 minutes, fully dissolve, cool the temperature to 50°C, then add papain with a concentrated solution quality of 3‰ to the concentrated solution, enzymatically hydrolyze it for 2 hours, and then add the concentrated solution with a quality of 2 ‰ of alkaline protease, hydrolyzed for 3h, to obtain enzymatic hydrolyzate; wherein, papain is the papain sold by Shanghai Shifeng Biotechnology Co., Ltd., and the neutral protease is the neutra...

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Abstract

A related method for extracting phosphatide and sea cucumber oil by utilizing a sea cucumber blanching liquid comprises: selecting a fresh sea cucumber blanching liquid as a raw material, performing reduced-pressure concentration, getting the concentrate and heating for dissolving, cooling and using protease for enzymatic hydrolysis; killing enzyme in the enzymatic hydrolysis liquid, employing a disc centrifuge for centrifuging, and collecting the enzymatic hydrolysis light liquid; performing hydration reaction on the enzymatic hydrolysis light liquid, and employing a disc centrifuge for secondary centrifuging, so as to obtain a precipitate and a light liquid; heating the light liquid, and performing vacuum drying, so as to obtain a sea cucumber oil crude extractive; and performing freeze drying on the precipitate, so as to obtain a sea cucumber phosphatide crude extractive. The extraction method is reasonable in technology, simple in operation and low in production cost, and is capable of reducing environmental pollution. The sea cucumber oil and the sea cucumber phosphatide extracted by using the method are high in nutrition and health-care value, and are widely applicable to food, health care and other fields.

Description

technical field [0001] The invention relates to mollusc aquatic food products, in particular to a method for extracting phospholipids and sea cucumber oil from sea cucumber blanching liquid. Background technique [0002] After fresh sea cucumbers are harvested, due to the presence of proteolytic enzymes such as autolytic enzymes, it is easy to peel and autolyze. In order to facilitate preservation, fresh sea cucumbers are usually processed and stored after being blanched in hot water to kill enzymes. Fresh sea cucumbers will produce a large amount of blanching liquid in the process of boiling in hot water. According to analysis and research, the sea cucumber blanching liquid contains a large amount of glycoprotein (ZL 2011 1 0384722.8), protein, saponin (imitation sea cucumber processing waste liquid) In addition to the separation and identification of several sea cucumber saponin compounds (Journal of Shandong University (Science Edition) 2011 No. 7), it is also rich in li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/325A23L1/327A23L1/28A23L1/29C07F9/10A23L17/00A23L17/20A23L33/00
CPCA23V2002/00C07F9/103A23V2200/30A23V2250/187A23V2250/1868A23V2250/2042A23V2300/14A23V2300/40
Inventor 袁文鹏刘昌衡张绵松孙永军胡炜鞠文明王志伟唐晓波
Owner SHANDONG HOMEY AQUATIC DEV
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