Polyamino acid-based elastic vascular tissue engineering scaffold and preparation method thereof

Abstract

The invention discloses a polyamino acid-based elastic vascular tissue engineering scaffold and a preparation method thereof. The inner layer is composed of ‑NH3+ in polysaccharides and ‑COO‑ in polyamino acids through electrostatic compounding, and the middle layer is chemically crosslinked. Scaffold layer; the outer layer is an electrospinning mesh, which is made of poly(ε-caprolactone)-b-polyamino acid ester block copolymer through an electrospinning method; the present invention simulates the normal blood vessel Complex structure and mechanical properties, on the one hand, the scaffold has good biocompatibility, and its compliance can be well matched with the host blood vessel; on the other hand, the intermediate layer scaffold has good elasticity, under the dynamic stimulation of the vascular bioreactor, the scaffold material It can well withstand the transmural pressure and the compliance of blood flow to the vessel wall; and the scaffold material deforms periodically, which can strengthen the remodeling of the mechanism itself, so that the regenerated blood vessel is closer to the normal blood vessel in terms of tissue composition, and has More excellent biomechanical properties.

Description

technical field [0001] The invention relates to a polyamino acid-based elastic blood vessel tissue engineering scaffold and a preparation method thereof. Background technique [0002] Cardiovascular disease is recognized as the main cause of morbidity and death. The World Health Organization announced in September 2011 that the proportion of death caused by cardiovascular disease was 30%. In the United States alone, 1 million people die from cardiovascular disease each year. Treatments for this disease include chemotherapy, angioplasty, stent insertion, resection of diseased tissue, and others. However, for patients, the success rate of this type of treatment is low, and there is a status quo of disease progression. At present, the most commonly used method is the reconstruction and replacement of blood vessels. However, due to the limitation of the source of autologous and allogeneic blood vessels, uncontrollable physical and chemical properties of tissues, severe inflamm...

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Problems solved by technology

However, such materials still cannot avoid degradation products that are acidic, immunogenic, and cause thromboembolism, and the regenerated vascular tissue lacks mechanical properties.

[0006] The fourth is to plant cells on biodegradable scaffolds for in vitro vascular regeneration. Although thromboembolism can be effectively avoided, the regenerated blood vessels lack mechanical strength.

Biomaterials (vol.31, 2010, p621-630) disclosed that under dynamic / static stimulation, smooth muscle cells were planted on non-woven polyglycolide porous scaffolds to successfully regenerate the vascular smooth muscle layer. Through histological and biomechanical analysis, dynamic stimulation The structure of the regenerated vascular smooth muscle layer is more similar to that of the normal vascular smooth muscle layer, but the material lacks elasticity

Therefore, it is still a huge challenge to prepare regenerated blood vessels similar to normal blood vessels. The key technology is due to the fact that the mechanical properties of scaffold materials cannot meet clinical needs.

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