Food enzyme preparation and a preparation method thereof
An enzyme preparation and food technology, applied in the field of food additives, can solve the problems of low thermal stability, high arsenic content, low enzyme activity preservation rate, etc., and achieve the effects of low fineness and high heat resistance
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Embodiment 1
[0024] Take by weighing 100 parts of Bacillus subtilis, 35 parts of Aspergillus niger, 55 parts of corn flour, 25 parts of ammonia, 40 parts of soybean cake, 15 parts of ammonium bicarbonate, 30 parts of alcohol, 50 parts of sulfuric acid, and 20 parts of ammonium sulfate. 5 parts of urea, 10 parts of maltose, 10 parts of calcium carbonate, 5 parts of sucrose, and 1 part of amyloglucosidase.
[0025] Put Bacillus subtilis, corn flour and soybean cake into the reaction kettle in turn, raise the temperature to 35°C, and react for 60 minutes, add calcium carbonate and Aspergillus niger, raise the temperature to 40°C, and react for 30 minutes.
[0026] Add sucrose and maltose, heat up to 120°C, react for 10 minutes, add ammonia, ammonium sulfate, ammonium bicarbonate and urea, react at 70°C for 40 minutes, then add alcohol and remaining materials, react at 50°C for 20 minutes, and precipitate.
[0027] The enzyme activity preservation rate of the product at room temperature for ha...
Embodiment 2
[0029] Take by weighing 100 parts of Bacillus subtilis, 55 parts of Aspergillus niger, 75 parts of corn flour, 45 parts of ammonia, 60 parts of soybean cake, 35 parts of ammonium bicarbonate, 50 parts of alcohol, 70 parts of sulfuric acid, and 40 parts of ammonium sulfate. 45 parts of urea, 30 parts of maltose, 50 parts of calcium carbonate, 25 parts of sucrose, and 20 parts of amyloglucosidase.
[0030] Put Bacillus subtilis, corn flour and soybean cake into the reaction kettle in sequence, raise the temperature to 55°C, and react for 100 minutes, add calcium carbonate and Aspergillus niger, raise the temperature to 60°C, and react for 50 minutes.
[0031] Add sucrose and maltose, heat up to 140°C, react for 30 minutes, add ammonia, ammonium sulfate, ammonium bicarbonate and urea, react at 90°C for 60 minutes, then add alcohol and remaining materials, react at 70°C for 40 minutes, and precipitate.
[0032] The enzyme activity preservation rate of the product at room temperatu...
Embodiment 3
[0034] Weigh 100 parts of Bacillus subtilis, 40 parts of Aspergillus niger, 60 parts of corn flour, 30 parts of ammonia, 45 parts of soybean cake, 20 parts of ammonium bicarbonate, 35 parts of alcohol, 55 parts of inorganic salt, ammonium sulfate 25 parts, 15 parts of urea, 15 parts of maltose, 20 parts of calcium carbonate, 10 parts of sucrose, 5 parts of amyloglucosidase.
[0035] Put Bacillus subtilis, corn flour and soybean cake in sequence in the reaction kettle, raise the temperature to 40°C, and react for 70 minutes, add calcium carbonate and Aspergillus niger, raise the temperature to 45°C, and react for 35 minutes.
[0036] Add sucrose and maltose, heat up to 125°C, react for 15 minutes, add ammonia, ammonium sulfate, ammonium bicarbonate and urea, react at 75°C for 45 minutes, then add alcohol and remaining materials, react at 55°C for 25 minutes, and precipitate.
[0037] The enzyme activity preservation rate of the product at room temperature for half a year is 95%, ...
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