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Preparation method of room-temperature preserved bifidobacteria enteric microcapsule

A bifidobacteria, room temperature preservation technology, applied in microcapsules, food preparation, medical preparations with inactive ingredients, etc., can solve the problems of reduced recovery rate, increased amount of capsule material, irregular shape of bacteria powder particles, etc. To achieve the effect of improving tolerance and heat resistance

Inactive Publication Date: 2015-04-29
内蒙古双奇药业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the fluidized bed coating process of the above two methods, the capsule core material is freeze-dried bacterial powder particles. Because the freeze-dried bacterial powder particles are loose in texture and irregular in shape, they are placed in the fluidized bed. Under the action of air flow, the violent impact between particles and particles, particles and pot body, particles and air will make the particles smaller and produce too much fine powder, which is adsorbed on the pot wall or shaking bag due to electrostatic effect , does not participate in fluidization, cannot be fully coated
At the same time, particles with too small particle size are easy to leak from the filter bag or bottom sieve, which reduces the recovery rate; Uneven coating thickness

Method used

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  • Preparation method of room-temperature preserved bifidobacteria enteric microcapsule
  • Preparation method of room-temperature preserved bifidobacteria enteric microcapsule
  • Preparation method of room-temperature preserved bifidobacteria enteric microcapsule

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1. Preparation method of bifidobacterium NQ-1501 enteric-coated microcapsules preserved at room temperature

[0020] The preparation method of bifidobacterium NQ-1501 enteric-coated microcapsules which can be stored at room temperature comprises the following steps:

[0021] 1) Prepare an enteric coating liquid composed of 40% polyacrylic acid resin latex, 1% triethyl citrate, 2% talcum powder and 57% purified water for subsequent use;

[0022] 2) Put 1.5kg of Bifidobacterium NQ-1501 bacteria powder in a fluidized bed coating machine, spray 500ml of 30% maltodextrin aqueous solution as a binder into the fluidized bed to prepare stable capsule core granules for later use ;

[0023] 3) Put the capsule core in a fluidized bed coating machine, first use 900ml of 30% starch aqueous solution as the coating liquid for bottom spray coating, and after spraying and drying, the primary microcapsules are obtained. Afterwards, 1875ml of polyacrylic acid resin enteric-coati...

Embodiment 2

[0025] Example 2. Bifidobacterium NQ-1501 enteric-coated microcapsule effect experiment

[0026] 1) Heat resistance test

[0027] 10 g of the freeze-dried bifidobacteria powder of Example 1 and the coated microcapsules were each placed in a sealed aluminum-plastic bag, and placed at 50° C. for 1, 4, 8, and 24 hours to determine the number of viable bacteria immediately. The result is as follows:

[0028]

[0029] In Example 1, the freeze-dried bacteria powder of bifidobacteria and the coated microcapsules were packed into sealed aluminum-plastic bags in an amount of 10 g per portion, stored at 25° C. for 8 weeks, and the results of the changes in the number of viable bacteria were as follows:

[0030]

[0031]

[0032] 2) gastric acid resistance test

[0033] Dissolve 1 g of the bifidobacterium freeze-dried powder and coated microcapsules in Example 1 in 100 ml of hydrochloric acid solution with a pH of 1.2, and measure the number of viable bacteria after bathing in...

Embodiment 3

[0036] Example 3. Preparation method of bifidobacterium NQ-1501 enteric-coated microcapsules preserved at room temperature

[0037] Concrete preparation method is identical with the technological process of embodiment 1, difference is as follows:

[0038] 1) Prepare an enteric coating solution composed of 30% polyacrylic acid resin latex, 2% triethyl citrate, 5% talcum powder and 63% purified water for later use.

[0039] 2) Evenly mix the bifidobacterium cells and the freeze-drying protective agent according to the mass ratio of 1:10, then vacuum freeze-dry, crush and pass through a 40-mesh sieve to obtain the bifidobacterium powder, and the number of live bacteria in the powder is 3.10×10 11 cfu / g.

[0040] 3) Put 2.0 kg of bifidobacterium powder in a fluidized bed coating machine, spray 750 ml of 40% maltodextrin aqueous solution as a binder into the fluidized bed to prepare stable capsule core granules for future use.

[0041] 4) Put the capsule core in a fluidized bed c...

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Abstract

The invention belongs to the technical field of probiotics product preparation, and particularly relates to a preparation method of a room-temperature preserved bifidobacteria NQ-1501 enteric microcapsule. The preparation method comprises the following steps: firstly, performing top spray and granulation to bifidobacteria NQ-1501 bacterial powder by taking maltodextrin as an adhesive through a fluidized bed coating machine, and then coating a capsule core with starch and polypropylic acid resin, to prepare the room-temperature preserved bifidobacteria NQ-1501 enteric microcapsule with two coatings. Through the technological flow of firstly granulating the bifidobacteria NQ-1501 bacterial powder and then coating, the problems that the coating recovery rate is low and the surface coating of the microcapsule is uneven in thickness due to direct coating of lyophilized bacterial powder can be solved, and further the normal-temperature preservation and intestinal-targeted release functions of the bifidobacteria NQ-1501 microcapsule can be realized.

Description

technical field [0001] The invention belongs to the technical field of preparation of probiotic products, and in particular relates to a method for preparing bifidobacterium NQ-1501 enteric-coated microcapsules which can be stored at room temperature. Background technique [0002] Bifidobacterium is a kind of gram-positive anaerobic bacteria, which has important physiological and health functions such as improving human immunity, improving intestinal function, lowering blood fat, protecting liver and anti-tumor. With the in-depth research on bifidobacteria, more and more bifido products have been promoted and applied in medical treatment, health care and nutrition. [0003] Because bifidobacteria are relatively fragile, they are easily affected by external factors such as temperature, pH, gas composition, and mechanical force, resulting in a decrease in the number of viable bacteria and a decrease in efficacy. Bifidobacterium products currently on the market, on the one han...

Claims

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Application Information

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IPC IPC(8): A61K35/745A61K47/32A61K9/50A23L1/29A23L33/00
Inventor 黄少磊刘彦民李康宁王春颖李晓军程超冯谦王世隆刘斌邢会霞龚虹李云旭王海霞马晓红袁虹马跃英王潇海棠
Owner 内蒙古双奇药业股份有限公司
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