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Arbuscular mycorrhizal fungi efficiently degrading atrazine and promoting plant growth and application of arbuscular mycorrhizal fungi

A technology to promote plant growth and arbuscular mycorrhizal fungi, applied in the direction of fungi, microorganism-based methods, microorganisms, etc., can solve the problems of limited biomass accumulation and achieve good results, high-efficiency degradation, and strong anti-interference Effect

Active Publication Date: 2015-04-29
南京蕃茂生物科技有限公司
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0005] The present invention aims to solve the problem that existing arbuscular mycorrhizal fungi for repairing atrazine-contaminated soil have limited accumulation of plant biomass, and provide an arbuscular mycorrhizal fungus that can efficiently degrade atrazine and effectively promote plant growth Mycorrhizal fungi and their applications

Method used

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  • Arbuscular mycorrhizal fungi efficiently degrading atrazine and promoting plant growth and application of arbuscular mycorrhizal fungi
  • Arbuscular mycorrhizal fungi efficiently degrading atrazine and promoting plant growth and application of arbuscular mycorrhizal fungi
  • Arbuscular mycorrhizal fungi efficiently degrading atrazine and promoting plant growth and application of arbuscular mycorrhizal fungi

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Embodiment 1

[0020] Such as Figure 1-3 As shown, an arbuscular mycorrhizal fungus that efficiently degrades atrazine and promotes plant production in this embodiment is the spore of Acaulospora lacunosa HDSF4, which is round or nearly round, and in the spore soil Solitary, growing laterally on conidia hyphae, orange-brown to dark brown, 80-130 μm in diameter; 2 layers of spore wall, W 1 It is colorless, 0.5μm thick, W 2 Orange-brown to dark brown, layered wall, the inner and outer surfaces are covered with regular or irregular conical and dish-shaped pits; the bud wall has 2 layers, the first layer is colorless membranous, 0.5 μm thick, and the second layer is colorless Membranous, with beaded patterns on the surface, 0.5-1 μm thick.

[0021] W in the spore wall of Acaulospora lacunosa (Acaulospora lacunosa) HDSF4 in the present embodiment 1 It is a perishable wall, which falls off when the spores mature, and has no reaction with Melzer's reagent; W 2 The wall thickness is 2 μm, the s...

Embodiment 2

[0025] Acaulospora lacunosa HDSF4 was screened in farmland soil contaminated by atrazine residues in Qiqihar.

[0026] In this implementation, the collection, screening and purification of Acaulospora lacunosa HDSF4 are carried out according to the following steps:

[0027] 1. Take the soil in the 2-20cm soil layer where the root circumference of the plant is polluted by the pesticide atrazine residues, use wet sieve decantation-sucrose centrifugation to separate the spores of different arbuscular mycorrhizal fungi from the soil, and separate the different arbuscular mycorrhizal fungi The root fungal spores were classified, and the dominant arbuscular mycorrhizal fungal spores were screened out; among them, the AM fungal dominance was divided into 4 grades according to the important value (I), I>50% was the dominant genus (species), 30 %<I≤50% is a subdominant genus (species), 10%<I≤30% is an associated genus (species), I≤10% is a rare genus (species);

[0028] Two, soak the ...

Embodiment 3

[0031]The expansion cultivation method of Acaulospora lacunosa (Acaulospora lacunosa) HDSF4 of the present embodiment comprises the following steps:

[0032] 1. Preparation of culture substrate: Weigh peat soil, frog stone and sand according to the volume ratio of 2:3:5, mix them evenly, and sterilize with high-temperature steam at 121°C for 1.5h;

[0033] 2. Expansion of strains: Germinate sorghum seeds, sow them on the culture substrate prepared in step 1, inoculate Acaulospora lacunosa HDSF4 strains at the same time, cover with soil 0.8-1.2cm, water and light After culturing for 4 months, the spores, mycelium fragments and host root system infected by the fungus containing Acaulospora lacunosa HDSF4 are obtained as the strains for propagation.

[0034] The spore density of Acaulospora lacunosa HDSF4 in the substrate obtained by the multiplication method of this embodiment is 20 spores / g soil.

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Abstract

The invention discloses an arbuscular mycorrhizal fungi efficiently degrading atrazine and promoting plant growth and an application of the arbuscular mycorrhizal fungi and relates to a fungi and an application of the fungi, in particular to an acaulospora arbuscular mycorrhizal fungi and an application of the acaulospora arbuscular mycorrhizal fungi. The problem that the existing arbuscular mycorrhizal fungi repairing atrazine polluted soil has limited accumulation action on plant biomass is solved. The arbuscular mycorrhizal fungi efficiently degrading the atrazine and promoting the plant growth is acaulospora lacunosa HDSF4, and preserved in China General Microbiological Culture Collection Center with a preservation number of CGMCC No. 9907 (China General Microbiological Culture Collection Center Number 9907). The acaulospora lacunosa HDSF4 is used for repairing the atrazine polluted soil, and tests prove the acaulospora lacunosa HDSF4 has a good effect and can effectively improve the amount of growth of plants in the atrazine polluted soil.

Description

technical field [0001] The invention relates to a fungus and its application, in particular to an arbuscular mycorrhizal fungus of the genus Asepsis and its application. Background technique [0002] Atrazine, also known as atrazine, is a selective systemic conduction pre-emergence and post-emergence herbicide, suitable for corn, sorghum, orchard, woodland, etc., and can control annual grass weeds and broad-leaved weeds, It also has a certain inhibitory effect on some perennial weeds. Due to its low cost and good weed control effect, atrazine is widely used in agricultural planting. And long-term use leads to a large amount of residues in the soil, which greatly reduces the yield of crops. The remediation of atrazine-contaminated soil can use physical, chemical and biological methods, because the physical treatment method only transfers the pollutants, cannot completely eliminate atrazine, and is expensive; while the chemical treatment method is likely to cause the contami...

Claims

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Application Information

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IPC IPC(8): C12N1/14B09C1/10C12R1/645
CPCB09C1/105C12N1/145C12R2001/645
Inventor 宋福强孙育新王辰郭夏立常伟范晓旭
Owner 南京蕃茂生物科技有限公司
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