The invention discloses a small-current single-phase earth fault line selection method based on spiking neural P systems. The method comprises the following steps: extracting zero sequence characteristics, comprising steady state and transient characteristics, of each line of a power distribution network at the fault moment, and establishing a fault measurement function of each line; obtaining theline selection criterion weight and line selection decision rule based on an information gain degree and a rough set theory; and performing fuzzy reasoning on the spiking neural P systems to obtain the fused fault measures, and determining the faulty line. According to the method disclosed by the invention, the fault information is fully utilized, the acquired steady state zero sequence current amplitude, steady state zero sequence current phase angle, zero sequence reactive power amplitude, zero sequence admittance amplitude, transient zero sequence current energy value and transient zero sequence current effective value are taken as the line selection criterions, the maximal fault measure is obtained by performing fuzzy reasoning on the spiking neural P systems, line selection results can be accurately obtained, the defect that a single line selection criterion cannot adapt to a complex fault condition is overcome, the small-current fault line selection precision is improved, and safe and reliable power supply of the power system is ensured.