Fucoidan sulfate and application of low-molecular-weight fucoidan sulfate in preparation of metabolic syndrome resistant medicines and health products

A fucoidan sulfate, low molecular weight technology, applied in the field of medicine, can solve the problems such as no application report of fucoidan sulfate

Inactive Publication Date: 2015-05-06
MARINE BIOMEDICAL RES INST OF QINGDAO CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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  • Fucoidan sulfate and application of low-molecular-weight fucoidan sulfate in preparation of metabolic syndrome resistant medicines and health products
  • Fucoidan sulfate and application of low-molecular-weight fucoidan sulfate in preparation of metabolic syndrome resistant medicines and health products
  • Fucoidan sulfate and application of low-molecular-weight fucoidan sulfate in preparation of metabolic syndrome resistant medicines and health products

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Experimental program
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Embodiment 1

[0026] The preparation method of fucoidan sulfate ester of the present invention comprises the following steps:

[0027] (1) Put the brown algae powder in an organic solvent such as methanol, acetone or ethanol with a mass ratio of 80%, soak each time at 80°C for 3 hours, repeat 3 times, to degrease by soaking;

[0028] The brown algae is taken from one or more of Fucus, Ascophyllum nodosum, Sargasso, Undaria pinnatifida, Hijiki, Sage, sea millet, kelp or kelp, among which Fucus is preferred.

[0029] (2) Extract with water at 80°C, then precipitate with ethanol with a mass ratio of 80%, and then dry at 40°C to obtain crude fucoidan sulfate;

[0030] (3) Then in CaCl 2 or MgCl 2 Precipitation with 20% ethanol in the presence of water-soluble alginate;

[0031] (4) Dialysis, concentration, precipitation with 80% ethanol by mass ratio, and drying at 40° C. to obtain purified fucoidan sulfate. The prepared fucoidan sulfate has a molecular weight of 200kD-2000kD (both values ​...

Embodiment 2

[0033] Example 2 Cytotoxicity detection of fucoidan sulfate (FS) and low molecular weight fucoidan sulfate (LFS).

[0034] The present invention adopts a classic cell model of metabolic syndrome, and evaluates the effect of the fucoidan sulfate on improving insulin resistance and anti-metabolic syndrome through experiments. Experimental results such as figure 1 As shown, fucoidan sulfate (FS) and low molecular weight fucoidan sulfate (LFS) have no obvious toxicity to cells in the range of 0-400 μg / mL, and when the concentration reaches 500 μg / mL and above, showed some cytotoxicity. However, the highest experimental concentration of the present invention is 100 μg / mL, so it will not produce any toxicity to cells.

Embodiment 3

[0035] Example 3 Fucoidan sulfate (FS) and low molecular weight fucoidan sulfate (LFS) inhibit the gluconeogenesis pathway of hepatocytes, increase glycogen synthesis, and improve insulin resistance.

[0036] The inhibitory effect of FS and LFS on the gluconeogenesis pathway of hepatocytes was evaluated by using the cell model established by the human hepatocyte line HepG2 exposed to high glucose and insulin-containing medium. Metformin was selected as the positive drug.

[0037] The experimental results are shown in Table 1. FS and LFS can significantly inhibit the gluconeogenesis level of liver cells and increase the amount of glycogen synthesis. The Western blot results in Table 2 also show that FS and LFS can significantly up-regulate insulin as shown in Table 2. Phosphorylation of IRS-2, Akt, GSK-3 in the signal transduction pathway, inhibits the expression of G6Pase. It was proved that FS and LFS can improve insulin resistance by specifically activating IRS-2 / PI3K signa...

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Abstract

The invention provides fucoidan sulfate and an application of low-molecular-weight fucoidan sulfate in preparation of metabolic syndrome resistant medicines and health products. By adopting fucoidan sulfate, insulin signaling pathways can be relatively well improved, gluconeogenesis can be inhibited, the glycogen synthesis quantity can be increased, the glucose absorption of the skeletal muscles can be promoted, and insulin resistance can be effectively improved. Fucoidan sulfate can be taken as a natural product or medicine to be used for preventing and treating insulin resistance and metabolic syndromes; and fucoidan sulfate is sourced from marine algae, has the advantages of rich resources, easiness in industrialization and high safety, and has wide market and application prospects on the aspects of preventing and treating the metabolic syndromes. Fucoidan sulfate and the application provided by the invention can be used for providing reference significance and enlightenments for high-value applications of the marine algae.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of fucoidan sulfate and low molecular weight fucoidan sulfate in the preparation of anti-metabolic syndrome medicines and health care products. Background technique [0002] Senile metabolic syndrome is a group of clinical syndromes with cardiovascular and cerebrovascular diseases, diabetes, hyperlipidemia, and hypertension, which seriously affect the health of the body. It is called the "death quartet". In recent years, with rapid economic development and changes in lifestyle and diet, the prevalence of metabolic syndrome in the elderly has risen sharply, and it has been widely recognized as a major health problem affecting human health all over the world. According to epidemiological data in my country, the prevalence rates of people over 45 years old and 55 years old are 2 times and 2.8 times that of people over 35 years old, respectively, and the ...

Claims

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Application Information

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IPC IPC(8): A61K31/737A61P3/00A23L1/30A23L33/10
Inventor 管华诗郝杰杰敦云楼周晓琳于广利夏萱
Owner MARINE BIOMEDICAL RES INST OF QINGDAO CO LTD
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