Preparation method for gel scaffold for repairing articular cartilage injuries

A technology for cartilage damage and joint repair, applied in medical science, prostheses, etc., can solve the problems of growth factor immunogenicity that cannot be used in vivo, gene transfection technology safety tumorigenicity, unfavorable differentiation of mesenchymal stem cells, etc. , to achieve the effects of clinical application, cartilage damage repair, light intensity and light time shortening

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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods have various limitations: growth factors cannot be used in vivo due to immunogenicity, there are safety and tumorigenic problems in gene transfection technology, and it is difficult to integrate tissue-engineered cartilage implants with original tissues, etc.
[0005] At present, most of the scaffold materials used in research and application are PLGA, PGA, collagen, fibrin, etc. These scaffold materials are characterized by being able to induce cells to produce a large amount of type I collagen instead of type II collagen, which is not conducive to mesenchymal Stem cells differentiate into chondrocytes
In the research field of drug sustained-release stents, most of the drugs used for sustained release are growth factors, which are expensive and immunogenic, and their application in vivo may bring the risk of animal-derived diseases

Method used

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  • Preparation method for gel scaffold for repairing articular cartilage injuries
  • Preparation method for gel scaffold for repairing articular cartilage injuries
  • Preparation method for gel scaffold for repairing articular cartilage injuries

Examples

Experimental program
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Embodiment 1

[0071] This embodiment is a method for preparing a gel scaffold for repairing articular cartilage damage, which specifically includes the following steps:

[0072] (1) Modification of sodium hyaluronate;

[0073] (2) Dissolve the PLGA nanoparticles of KGN in the sodium hyaluronate modified in step (1) at a concentration of 10um / L, and then add the photoinitiator Irgacure 2595 to obtain the slow-release small molecule compound-sodium hyaluronate gel Glue compound system, by weight, the amount of photoinitiator added is 1 / 9 of the small molecular compound;

[0074] (3) Inject / fill the slow-release small molecule compound-sodium hyaluronate gel complex system into the articular cartilage damage to be repaired and irradiate with ultraviolet point light source, 20 μW / cm 2 The ultraviolet point light source was irradiated for 20 seconds to obtain a gel scaffold for repairing articular cartilage damage.

[0075] The preparation method of the PLGA nanoparticles of the KGN comprises ...

Embodiment 2

[0081] This embodiment is a method for preparing a gel scaffold for repairing articular cartilage damage, which specifically includes the following steps:

[0082] (1) Modification of sodium hyaluronate;

[0083] (2) Dissolve the PLGA nanoparticles of KGN in the sodium hyaluronate modified in step (1) at a concentration of 2um / L, and then add the photoinitiator Irgacure 2595 to obtain the slow-release small molecule compound-sodium hyaluronate gel Glue compound system, by weight, the amount of photoinitiator added is 1 / 10 of the small molecule compound;

[0084](3) Inject / fill the slow-release small molecule compound-sodium hyaluronate gel complex system into the articular cartilage damage to be repaired and irradiate with ultraviolet point light source, 10 μW / cm 2 The ultraviolet point light source was irradiated for 40 seconds to obtain a gel scaffold for repairing articular cartilage damage.

[0085] The preparation method of the PLGA nanoparticles of the KGN comprises th...

Embodiment 3

[0091] This embodiment is a method for preparing a gel scaffold for repairing articular cartilage damage, which specifically includes the following steps:

[0092] (1) Modification of sodium hyaluronate;

[0093] (2) Dissolve the PLGA nanoparticles of KGN in the sodium hyaluronate modified in step (1) at a concentration of 60um / L, then add the photoinitiator Irgacure 2595 to obtain the slow-release small molecule compound-sodium hyaluronate gel Glue compound system, by weight, the amount of photoinitiator added is 1 / 8 of the small molecule compound;

[0094] (3) Inject / fill the slow-release small molecule compound-sodium hyaluronate gel complex system into the articular cartilage damage to be repaired and irradiate with ultraviolet point light source, 6 μW / cm 2 The ultraviolet point light source was irradiated for 1 min to obtain a gel scaffold for repairing articular cartilage damage.

[0095] The preparation method of the PLGA nanoparticles of the KGN comprises the followi...

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Abstract

The invention relates to the fields of medicines and medical instruments, and particularly relates to a preparation method for a gel scaffold for repairing articular cartilage injuries. The preparation method specifically comprises the following steps: (1) modifying sodium hyaluronate; (2) dissolving PLGA nano-particles of a controlled-release small molecular compound in the sodium hyaluronate modified in the step (1), and then adding with a photoinitiator to obtain a controlled-release small molecular compound-sodium hyaluronate gel compound system; and (3) injecting/filling the controlled-release small molecular compound-sodium hyaluronate gel compound system to the articular cartilage injuries to be repaired for being irradiated by an ultraviolet point light source, so as to obtain the gel scaffold for repairing articular cartilage injuries. The gel scaffold provided by the invention is high in plasticity, and capable of being randomly shaped according to the shapes of cartilage defects; harmful substances are not introduced in the whole process, and the gel scaffold can be used in vivo.

Description

technical field [0001] The invention relates to the field of medicines and medical devices (interventional medical devices), in particular to a method for preparing a gel support for repairing articular cartilage damage. Background technique [0002] As my country enters an aging society, more and more patients suffer from knee cartilage damage. There is currently no good treatment for this disease. At present, subchondral bone drilling, osteochondral transplantation and autologous chondrocyte transplantation commonly used in clinical practice can only partially relieve the symptoms, and cannot achieve the purpose of reconstructing cartilage defects. Compared with the type II collagen of the original hyaline cartilage, the biological characteristics are far different. [0003] The basic principle of regenerating and repairing tissue at the cartilage defect and rebuilding the cartilage defect is: to recruit self or introduce exogenous stem cells, compare primitive cells to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/52A61L27/20A61L27/18A61L27/54
Inventor 顾臻史冬泉
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