A method for preparing biomacromolecule-coated microspheres by microwave heating

A biomacromolecule and microwave heating technology, applied in the field of materials, can solve the problems of poor dispersion and uneven product particle size, and achieve the effect of high microwave absorption rate

Active Publication Date: 2016-08-31
武汉纳达康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the above defects or improvement needs of the prior art, the purpose of the present invention is to provide a method for preparing biomacromolecule-coated microspheres by microwave heating, wherein the key cross-linking and coagulation steps, heating means, temperature setting, etc. are improved , compared with the existing technology, it can effectively solve the problem of uneven particle size and poor dispersion of the product; and the preparation method is flexible in operation, and can adjust parameter settings for different biomacromolecule / microsphere systems, thickness requirements of the coating layer, etc. , a wide range of applications

Method used

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  • A method for preparing biomacromolecule-coated microspheres by microwave heating
  • A method for preparing biomacromolecule-coated microspheres by microwave heating
  • A method for preparing biomacromolecule-coated microspheres by microwave heating

Examples

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Effect test

Embodiment 1

[0058] In this example, superparamagnetic nanoparticles coated with bovine serum albumin (BSA) were prepared by microwave heating. The frequency of microwave is 2450MHz. The magnetic particles used are Fe prepared by co-precipitation method 3 o 4 Superparamagnetic nanoparticles (SPIO, particle size 7-20nm) were treated with citric acid and washed with PBS buffer solution for later use. The thermal denaturation temperature of the BSA used is 72-74° C. when heated for 30 minutes under the condition of 0.1% PBS buffer solution (obtained by measuring the particle size change of the BSA solution at different temperatures by dynamic light scattering method).

[0059] Concrete preparation steps are as follows:

[0060] 1. Take 5 g of bovine serum albumin BSA and dissolve it in 500 mL of PBS buffer solution, the concentration of the PBS buffer solution is 0.1 mol / L. Add 0.5g of SPIO particles prepared in advance by co-precipitation method, the ratio of BSA to SPIO is 10:1, and mix...

Embodiment 2

[0064] 1. Get 2kg of bovine serum albumin BSA and dissolve it in 500L of PBS buffer solution (to make the whole system more stable). The concentration of the PBS buffer solution is 0.05mol / L. Add 2kg of SPIO particles prepared in advance by co-precipitation method, the ratio of BSA to SPIO is 1:1, and mix at room temperature for 12 hours at a speed of 60rpm on a roller bottle to make BSA adhere to the surface of magnetic particles as much as possible.

[0065] 2. Put the above solution into a microwave heater to heat. The overall temperature of the solution is preset to 63°C, the total heating time is set to 35 minutes, the maximum microwave power is set to 1800W, and the heating method is pulse heating. The duty cycle of the heating pulse signal is automatically determined by the PID temperature controller through the AT self-tuning function. adjust. For a schematic diagram of the device used, see image 3 . The stirrer rotates at 200 rpm during the heating process.

[00...

Embodiment 3

[0068] 1. Get 10kg bovine serum albumin BSA and dissolve it in 500L PBS buffer solution, the concentration of the PBS buffer solution is 0.1mol / L. Add 100g of SPIO particles prepared in advance by co-precipitation method, the ratio of BSA to SPION is 100:1, and mix at room temperature at 40rpm on a roller bottle for 24 hours to make BSA adhere to the surface of magnetic particles as much as possible.

[0069] 2. Put the above solution into a microwave heater to heat. The overall temperature of the solution is preset to 63°C, the total heating time is set to 60 minutes, the maximum microwave power is set to 5000W, and the heating method is pulse heating. The duty cycle of the heating pulse signal is automatically determined by the PID temperature controller through the AT self-tuning function. adjust. The stirrer rotates at 100 rpm during the heating process.

[0070] 3. After the reaction is complete, cool the solution to room temperature. Use a magnetic separator to suck t...

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Abstract

The invention discloses a method for preparing biological macromolecule coated microspheres through microwave heating. The method comprises the following steps: performing crosslinking coagulation on biological macromolecules on the particle surface by adopting a microwave pulse heating mode to form core-shell type microspheres coated by the biological macromolecules; and maintaining the properties of other biological macromolecules in the biological macromolecule / particle mixed solution system invariable, and orderly controlling formation of the core-shell type microspheres coated by the biological macromolecules. By utilizing the anisotropy of inorganic nanometer materials on the microwave absorption rate, the duty ratio of microwaves is adjusted, so that the crosslinking coagulation of the biological macromolecules is orderly and stably performed, and the thickness of the biological macromolecule coating layer can be flexibly controlled; the preparation method is simple, and the prepared core-shell type microspheres are low in nonspecific adsorption, high in biocompatibility and easy to couple and label and can be widely applied to the fields of labeled immunoassay, immunochromatography, bio-labeling and separation and purification.

Description

technical field [0001] The invention belongs to the field of materials, and more specifically relates to a method for preparing biomacromolecule-wrapped microspheres by microwave heating. Background technique [0002] In recent years, the application of inorganic nanomaterials (such as quantum dots, superparamagnetic nanoparticles, fluorescent upconversion nanocrystals, etc.) in biomedicine has become a research hotspot in the scientific and industrial circles. In order to apply inorganic nanomaterials to biomedicine, it is first necessary to solve the problems of water solubility, stability, modifiability and biocompatibility of inorganic microparticles. At present, the most commonly used method is to wrap inorganic particles with synthetic polymers such as polystyrene to form stable core-shell microspheres, and then increase the water solubility and modifiability of polymer microspheres through surface chemical modification and other methods. Although the polymer-encapsul...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J13/02B01J13/14
CPCB01J13/04B01J13/14
Inventor 杨海栗静张卓玲汪紫晶金伟周琦冰杨祥良
Owner 武汉纳达康生物科技有限公司
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