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The invention discloses 1-(4-aminophenyl)-3-[(4-aminophenyl) carbamoylamino] urea as well as a preparation method and application thereof. The 1-(4-aminophenyl)-3-[(4-aminophenyl) carbamoylamino] urea product is obtained by reacting p-phenylenediamine and diethyl azodicarboxylate under the action of a catalyst. The method is simple in process, milder in reaction condition, easier to control, high in environmental protection property, low in danger coefficient, high in yield and more suitable for industrial application. The product can interact with carbon black to improve the dispersion of carbon black with high specific surface area, can react with carbon-carbon double bonds in natural rubber to form a stable cross-linked network, improves the anti-reversion performance of a rubber material, and has more important social significance and higher economic value.