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The new strain t16 of authentic astragalus nitrogen-fixing bacteria and the preparation method of its fermented liquid

A technology of authentic astragalus and new strains, applied in microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of low nitrogen fixation efficiency and special nitrogen fixation, and achieve convenient fermentation conditions, easy preservation and preparation process of strains simple effect

Inactive Publication Date: 2017-12-15
SHANXI AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among the three types of nitrogen-fixing bacteria mentioned above, self-fixing nitrogen-fixing bacteria are not suitable for widespread application in agriculture due to their low nitrogen-fixing efficiency and special nitrogen-fixing. Therefore, combined nitrogen-fixing bacteria and symbiotic nitrogen-fixing bacteria are mainly used in agriculture at present.

Method used

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  • The new strain t16 of authentic astragalus nitrogen-fixing bacteria and the preparation method of its fermented liquid
  • The new strain t16 of authentic astragalus nitrogen-fixing bacteria and the preparation method of its fermented liquid
  • The new strain t16 of authentic astragalus nitrogen-fixing bacteria and the preparation method of its fermented liquid

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Effect test

Embodiment 1

[0045] A genuine Astragalus nitrogen-fixing bacteria ( Rhizobium sp.) The new strain t16 was preserved in the General Microorganism Center of China Committee for Microorganism Culture Collection on September 18, 2014, and it was classified as Rhizobium Rhizobium sp., the preservation number is CGMCC No. 9676, address: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

[0046] Authentic astragalus nitrogen-fixing bacteria of the present invention ( Rhizobium sp.) The new strain t16 was isolated from the growth soil of Astragalus membranaceus in Hunyuan area, Shanxi province. Put the new strain t16 on the Ashby nitrogen-free medium, and the colonies of the new strain t16 are round, white, translucent and moist on the YMA plate (see figure 1 ). Gram stain showing the new strain t16 as a Gram-negative bacilli (see figure 2 ), malachite green staining was negative, suggesting that the cells of the new s...

Embodiment 2

[0049] The preparation method of authentic astragalus nitrogen-fixing bacteria new bacterial strain t16 fermented liquid of the present invention, comprises the steps:

[0050] (1) Seed liquid preparation Pick the new strain t16 from the slant of the test tube with an inoculation needle and put it on the slant medium (the slant made by adding 1.5% agar to the YMA liquid medium), cultivate it at a constant temperature of 28°C for 24 hours, and inoculate it into a 100mL container. In the Erlenmeyer flask of the LB culture medium, 180r / min shake culture 12h, make seed liquid;

[0051] (2) Fermentation broth preparation: Incorporate the seed liquor prepared in (1) into the fermentation medium at a ratio of 1% by volume. The culture conditions are: 28°C, initial pH 6.8-7.0, shaker speed 180-200r / min Cultivate for 30 hours to obtain a fermentation broth.

[0052] The fermentation medium is composed of the following mass-volume ratios: 14 parts of mannitol, 5 parts of yeast powder, ...

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Abstract

The invention discloses an authentic new strain t16 of Rhizobium sp., which was preserved on September 18, 2014 in the General Microorganism Center of China Committee for the Collection of Microorganisms, and the preservation number is CGMCC No.9676. The present invention describes in detail the morphological characteristics, physiological and biochemical indicators, and 16S rDNA sequence determination results of the new strain t16 of the authentic Astragalus azotobacter (Rhizobium sp.). The nitrogen fixation test shows that the fermentation liquid of the new strain t16 of the authentic Astragalus azotobacter (Rhizobium sp.) of the present invention has a better nitrogen fixation effect on the authentic Astragalus membranaceus, and can effectively provide the natural nitrogen source requirements for the growth of the authentic Astragalus membranaceus, thereby improving the The natural quality of authentic Astragalus provides the corresponding source of nutrition.

Description

technical field [0001] The invention relates to the utilization field of plant nitrogen-fixing bacteria, in particular to a preparation method of authentic traditional Chinese medicine astragalus nitrogen-fixing bacteria t16 and a fermented liquid. Background technique [0002] In recent years, the fertilizer waste, soil and groundwater pollution, and ecological imbalance caused by the global planting industry's over-reliance on and large-scale application of chemical fertilizers have attracted widespread attention from all over the world. Reducing the use of chemical fertilizers and increasing the use efficiency of nitrogen fertilizers are important issues that need to be solved urgently in current agricultural production. Giving full play to the biological nitrogen fixation of nitrogen-fixing bacteria is the most effective way to reduce the use of nitrogen fertilizers. The research, development and utilization of biological nitrogen fixation resources play an important rol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08
CPCC12N1/205C12R2001/01
Inventor 唐中伟梁建萍周然薛智权李浩李倩
Owner SHANXI AGRI UNIV
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