Method for preparing peptone from enzymatically-hydrolyzed earthworm dry powder and purpose of peptone

A technology for preparing peptone and earthworm powder, which is applied in bacteria, fermentation and other directions, can solve the problem of not finding protein by enzymatic hydrolysis of earthworm powder, and achieve the effects of rich amino acid species, good effect and high protein content

Inactive Publication Date: 2015-05-06
高向阳
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Method for preparing peptone from enzymatically-hydrolyzed earthworm dry powder and purpose of peptone
  • Method for preparing peptone from enzymatically-hydrolyzed earthworm dry powder and purpose of peptone

Examples

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Effect test

Embodiment 1

[0018] The preparation method of peptone prepared by enzymatic hydrolysis of earthworm powder:

[0019] Degreasing of earthworm powder: take earthworm powder, pre-dry in a dryer at room temperature for 10-15 minutes, use Soxhlet extraction system, and extract with petroleum ether for 25-30 minutes at 45°C-55°C. The liquid ratio is earthworm powder: petroleum ether = 1g: 5~10ml, rinse with petroleum ether for 25~30min, and after degreasing, place the sample in a fume hood to fully evaporate to dry to obtain degreased earthworm powder.

[0020] Fully wash fresh earthworms with clean water to remove surface impurities, soak them in 0.1% salt water for 15-20 minutes, and wash them with clean water after soaking. Pour the washed fresh worms into boiling water at 90°C~100°C for 1~2min. After the completion, remove the filtered water, put them in an oven, and dry them at 80°C~100°C for 6~8 hours to obtain dried earthworms. Earthworm powder was crushed with a pulverizer, and its wate...

Embodiment 2

[0025] Experiments on cultivating Escherichia coli and lactic acid bacteria with earthworm peptone:

[0026] Competitor (LB medium) content (g / 100ml) Content of medium prepared by earthworm peptone of the present invention (g / 100ml) Peptone 1.0 - Peptone of the present invention - 1.0 Yeast extract 0.5 0.5 NaCl 1.0 1.0

[0027] Competitor (MRS medium) content g / 100ml) Content of medium prepared by earthworm peptone of the present invention (g / 100ml) casein peptone 1.0 - Homemade Peptone - 1.0 Beef Extract 1.0 1.0 yeast extract 0.5 0.5 glucose 0.5 0.5 sodium acetate 0.5 0.5 Diammonium citrate 0.2 0.2 Tween 80 0.1 0.1 Dipotassium phosphate 0.2 0.2 Magnesium Sulfate Heptahydrate 0.02 0.02 Manganese Sulfate Heptahydrate 0.005 0.005

[0028] Note: "-" means no such substance

[0029] The comparison of the OD value and viable count results bet...

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Abstract

A provided method for preparing peptone from an enzymatically-hydrolyzed earthworm dry powder comprises: (1) getting earthworm powder, under the condition of 45 DEG C-55 DEG C, utilizing a Soxhlet extraction system to perform extraction for 25-30 min and leaching for 25-30 min by using petroleum ether, and drying to obtain degreased earthworm powder; and (2) adding the degreased earthworm powder into a citric acid-sodium citrate buffer with pH of 4-8, heating to 40 DEG C-80 DEG C, adding trypsin accounting for 2%-5% by weight of the earthworm powder for enzymatic hydrolysis for 1-4 h, after enzymatic hydrolysis is finished, performing enzyme deactivation on the solution at 90 DEG C-100 DEG C for 15-20 min, centrifuging, adjusting pH of the supernatant obtained through centrifugation to be 5.5-7.5, and performing reduced-pressure drying, so as to obtain peptone dry powder. The method does not destroy amino acid compositions in earthworm powder, and is simple in technological operation, high in hydrolysis efficiency and small in environmental pollution. The prepared peptone has good effect when being applied to culture escherichia coli and lactic acid bacterium, and is applicable as a microbe medium.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing peptone by enzymolyzing earthworm dry powder and its application. Background technique [0002] Earthworms, also known as earthworms, belong to the phylum Oligochaeta, and there are many types. Eisenia chinensis, which produces earthworm meat and worm castings, belongs to the family Orthowormidae, such as the "Daping No. 2" earthworm and "Beixing No. 2" earthworm introduced from Japan in 1979. These two species belong to Eisenia chinensis. It has strong adaptability, high reproductive rate, and is suitable for artificial breeding. The protein content of earthworms is very high, accounting for about 8% to 10% of fresh weight and 50% to 67% of dry matter. It contains 17 kinds of amino acids, and the essential amino acids are complete. It is a high-quality protein and can be used as a food additive with cyanobacteria protein comparable. The lipid co...

Claims

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Application Information

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IPC IPC(8): C12P21/06C12N1/20
Inventor 高向阳冯俊杰林伟民连晓东
Owner 高向阳
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