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Preparation method of microorganism-nano particle composite system used for degrading phenol

A nanoparticle and microbial technology, applied in chemical instruments and methods, biological water/sewage treatment, water pollutants, etc., can solve the problems of lack of synergy between microorganisms and nanomaterials, complex preparation methods, and difficulty in promotion. The effect of overcoming the difficulty of desorption, simple preparation method and accelerating the biodegradation process

Active Publication Date: 2015-05-13
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its preparation method is too complicated, and the synergistic effect of microorganisms and nanomaterials has not been brought into play, so it is difficult to promote

Method used

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  • Preparation method of microorganism-nano particle composite system used for degrading phenol
  • Preparation method of microorganism-nano particle composite system used for degrading phenol
  • Preparation method of microorganism-nano particle composite system used for degrading phenol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Composite system of microorganisms and nanoparticles for degrading phenol

[0036] (1) Introduce polymer into hollow porous silica (HMS)

[0037]Dissolve 2ml of γ-aminopropyltriethoxysilane and 0.8g of succinic anhydride in 10ml of dimethylsulfoxide, then disperse 50mg of hollow porous silica (HMS) into this solution, stir at room temperature for 5h, centrifuge, The precipitate was vacuum-dried to obtain surface-modified hollow porous silica HMS-COOH; the obtained HMS-COOH nanoparticles were dispersed to contain 113 mg N-isopropylacrylamide, 1 mmol N,N-methylenebisacrylamide, 1.0 mmol methyl Acrylamide and 7mg of azobisisobutyronitrile in 10ml of dimethyl sulfoxide, stirred for 12 hours, let the monomer and initiator small molecules enter the cavity of the nanoparticle; centrifuge to remove the supernatant, and redisperse the remaining precipitate Into 10 ml dimethyl sulfoxide, under the protection of nitrogen, polymerize at 70°C for 7 hours, centrifuge, prec...

Embodiment 2

[0046] Disperse 50 mg TRPHMS and HMS separately to 20 ml 500mg L -1 In the phenol solution, samples were taken at different times to measure the adsorption capacity. The TRPHMS adsorbed to saturation was obtained by centrifugation, and then placed in clear water at different temperatures to test the concentration of phenol in the water, so as to determine the desorption of phenol from nanoparticles.

[0047] attached image 3 The adsorption of TRPHMS and HMS above and the desorption of TRPHMS at different temperatures (below and above the nanoparticle LCST (lower critical solution temperature)), it can be clearly seen from the figure that compared with HMS, the introduction of polymer After TRPHMS adsorption rate and adsorption capacity are increased. In addition, in the case of desorption, after the temperature rises (greater than LCST), the desorption speed of TRPHMS is faster than when the temperature is lower than LCST, indicating that the hollow porous nanoparticles of ...

Embodiment 3

[0049] Divide 150 ml of Pseudomonas putida LB culture solution into three equal portions, two of which were grafted with TRPHMS according to the above example, and the other portion was centrifuged for later use. Add two parts of BacteriaTRPHMS and pure bacterial liquid Bacteria to 100 ml 500 mg L -1 The phenol solution was made into three samples; the pure bacterial solution and one BacteriaTRPHMS sample were placed at room temperature; the other BacteriaTRPHMS sample was placed alternately at room temperature and in a 37°C water bath; samples were taken separately to test the concentration of phenol at different times. The steps of alternating water bath treatment are 1h at room temperature and 20 min in 37°C water bath

[0050] attached Figure 4 is the variation of phenol concentration in different samples at different times. It can be seen from the figure that the composite system degrades phenol faster than the simple bacteria, which is due to the adsorption of nanopar...

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Abstract

The invention discloses a preparation method of a microorganism-nano particle composite system used for degrading phenol. The preparation method comprises the following steps: at first, introducing a temperature sensitive polymer into the hollow cavities of hollow porous silica nanoparticles; then grafting the composite nanoparticles onto the surfaces of bacteria. Under the condition of a lower temperature, the composite nanoparticles can adsorb phenol from liquid waste and can release the adsorbed phenol molecules at a higher temperature, by means of this process, the relative concentration of phenol around the bacteria increases, so as to speed up the metabolism process. The phenol is completely degraded by the microbial metabolism and is converted into carbon dioxide and water, and no secondary pollution is generated. The temperature sensitive polymer is introduced into the hollow cavities of the hollow porous silica nanoparticles to increase the adsorbing capacity of the nanoparticles on one hand, and the adsorption and desorption of the phenol on the nanoparticles can be achieved at different temperatures on the other hand. The invention provides a wider selection for wastewater treatment agents.

Description

technical field [0001] The invention belongs to the technical field of polymer nano-composite materials, and in particular relates to a preparation method of a temperature-responsive nano-composite compounded with microorganisms and an application in phenol degradation. Background technique [0002] Phenol is a common chemical raw material. With the development of the national economy and the expansion of the chemical industry, phenol has become one of the common pollutants in sewage. In sewage treatment, the removal of phenol has also become a routine task. Due to the high solubility of phenol in water, wastewater containing phenol is considered to be one of the most difficult wastewater to treat. At present, the methods for treating phenol-containing wastewater mainly include adsorption, chemical oxidation, and biological methods. Among them, microorganisms can degrade phenol into carbon dioxide and water without secondary pollution, so the biological method is recognized...

Claims

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Application Information

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IPC IPC(8): C02F3/34C02F101/34
CPCC02F3/34C02F2101/345C02F2103/34
Inventor 路建美陈冬赟杨舜
Owner SUZHOU UNIV
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