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A horse-faced fish skin collagen antioxidant peptide and preparation method thereof

A technology of antioxidant peptides and collagen, applied in chemical instruments and methods, animal/human proteins, specific peptides, etc., can solve problems such as the influence of antioxidant peptide activity, reduced antioxidant performance, poor thermal stability, etc., to improve resources The effect of utilization rate, high antioxidant activity, and good thermal stability

Active Publication Date: 2018-02-23
ZHEJIANG WANLI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the antioxidant peptides in the prior art generally have poor thermal stability, and the activity of antioxidant peptides is easily affected severely after digestion in the gastrointestinal environment, thereby reducing their antioxidant properties
[0004] Horse-faced fish, also known as skinned fish and rubber fish, is a marine economic fish second only to hairtail in my country, while the edible part of horse-faced fish is less, and the inedible part of horse-faced fish accounts for about 53%, of which fish skin accounts for 8.5% to 9.4%, the skin of horse noodles is thick and hard, and the fish skin is usually peeled off and discarded during processing, which not only pollutes the environment, but also causes a waste of resources
In fact, horse-faced fish skin is rich in collagen. If it is developed and utilized to prepare active peptides, significant economic and social benefits will be obtained. However, in the prior art, collagen is prepared from horse-faced fish skin. The technology of protein antioxidant peptide is rarely reported

Method used

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  • A horse-faced fish skin collagen antioxidant peptide and preparation method thereof
  • A horse-faced fish skin collagen antioxidant peptide and preparation method thereof
  • A horse-faced fish skin collagen antioxidant peptide and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0037] In the present embodiment, the preparation method of horse-faced fish skin collagen antioxidant peptide comprises the following steps:

[0038] (1) Pretreatment of horse-faced fish skin

[0039] Add the washed and minced horse-faced fish skin into isopropanol with a volume concentration of 5% according to the solid-to-liquid weight ratio of 1:20, soak and degrease for 10 hours, filter to remove isopropanol, wash the filter residue with distilled water, and then remove the filter residue Add the NaCl solution with a mass concentration of 10% according to the solid-liquid weight ratio of 1:5, filter after soaking for 12 hours, and wash the obtained filter residue with distilled water for later use;

[0040] (2) Preparation of horse-faced fish skin collagen

[0041] The filter residue obtained at the end of step (1) was added into acetic acid with a concentration of 0.5 mol / L and soaked for 12 hours at a solid-to-liquid weight ratio of 1:10, and then centrifuged at 10,000...

Embodiment 2

[0050] In the present embodiment, the preparation method of horse-faced fish skin collagen antioxidant peptide comprises the following steps:

[0051] (1) Pretreatment of horse-faced fish skin

[0052] Add the washed and minced horse-faced fish skin into isopropanol with a volume concentration of 15% according to the solid-to-liquid weight ratio of 1:10, soak and degrease for 20 hours, filter to remove isopropanol, wash the filter residue with distilled water, and then remove the filter residue Add the NaCl solution with a mass concentration of 10% according to the solid-liquid weight ratio of 1:10, filter after soaking for 24 hours, and wash the obtained filter residue with distilled water for subsequent use;

[0053] (2) Preparation of horse-faced fish skin collagen

[0054] The filter residue obtained at the end of step (1) was added into acetic acid with a mass concentration of 0.1mol / L according to the solid-to-liquid weight ratio of 1:30 and soaked for 36h, and then cen...

Embodiment 3

[0063] In the present embodiment, the preparation method of horse-faced fish skin collagen antioxidant peptide comprises the following steps:

[0064] (1) Pretreatment of horse-faced fish skin

[0065] Add the washed and minced horse-faced fish skin into isopropanol with a volume concentration of 10% according to the solid-liquid weight ratio of 1:15, soak and degrease for 16 hours, filter to remove isopropanol, wash the filter residue with distilled water, and then remove the filter residue Add the NaCl solution with a mass concentration of 8% according to the solid-liquid weight ratio of 1:7, filter after soaking for 18 hours, and wash the obtained filter residue with distilled water for later use;

[0066] (2) Preparation of horse-faced fish skin collagen

[0067] Add the filter residue obtained at the end of step (1) into acetic acid with a concentration of 0.3mol / L at a solid-to-liquid weight ratio of 1:20 and soak for 25h, then centrifuge at 9000r / min to collect the sup...

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Abstract

The invention relates to a jerk filefish skin collagen antioxidative peptide which is characterized in that the amino acid sequence of the antioxidative peptide is Asn-Glu-Gly-Ala-Cys-Gly, and the molecular ion peak detected by UPLC-MS is [M]+531m / z. The invention further relates to a preparation method of the jerk filefish skin collagen antioxidative peptide. According to the method provided by the invention, a double-enzyme fractional hydrolysis technique is combined with an ultrafiltration and gel column chromatography technique, the whole preparation process is simple and easy to control and high in hydrolysis efficiency, and the obtained antioxidative peptide is safe and free of side effects, has relatively high antioxidant activity, and not only has a good eliminating effect to DPPH.free radicals, but also has relatively good thermal stability. The antioxidative peptide digested by the gastrointestinal digestive tract still maintains relatively high antioxidant activity. In addition, the raw materials adopted by the antioxidative peptide prepared are processing wastes of a marine low-value fish jerk filefish, so that the environmental pollution is effectively avoided and the utilization ratio of resources is improved.

Description

technical field [0001] The invention relates to a horse-faced fish skin collagen antioxidant peptide, and also relates to a preparation method of the horse-faced fish skin collagen antioxidant peptide. Background technique [0002] Oxidation is one of the important causes of human aging phenomenon, this view has been confirmed. Studies have shown that cancer, aging or other diseases are mostly related to excessive free radicals. Therefore, how to resist oxidation in the human body has become one of the main directions of research and development of health care products and cosmetics. Oxidants have become a research hotspot in recent years. [0003] Studies have found that the peptides obtained by protein hydrolysis have good antioxidant activity, and are small in molecular weight, easy to absorb, safe and reliable. Therefore, antioxidant peptides have good application prospects in many fields such as food processing, health products, and cosmetics. The Chinese invention pa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/78C12P21/06C07K1/36C07K1/34C07K1/16
CPCC07K14/78
Inventor 曹少谦戚向阳柳余莉杨华
Owner ZHEJIANG WANLI UNIV