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Spirulina culture medium

A culture medium and technology of spirulina, applied in the direction of microorganism-based methods, microorganisms, bacteria, etc., can solve problems such as waste and increased production costs, and achieve the effects of reducing production costs, high survival rate, and improving cultivation efficiency

Active Publication Date: 2015-05-13
NINGBO FUTIAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a constant flow of gas may cause CO 2 The waste; the use of real-time monitoring pH meter will also lead to an increase in production costs

Method used

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  • Spirulina culture medium
  • Spirulina culture medium
  • Spirulina culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0011] (1) Prepare the spirulina medium with the following formula in fresh water: sodium bicarbonate 16.8g / L, superphosphate 0.5g / L, potassium chloride 0.5g / L, sodium chloride 1g / L, magnesium chloride 0.2g / L , ferric ammonium citrate 15mg / L, N 2 EDTA 10mg / L, methyl naphthalene acetate, 0.5mg / L, VB 1 0.006mg / L, VB 12 0.05mg / L, ZnSO 4 4H 2 O 23μg / L, MnCl 2 4H 2 O 178μg / L, CuSO 4 ·5H 2 O10μg / L, Na 2 MoO 4 2H 2 O 7.3 μg / L, CoCl 2 ·6H 2 O 12μg / L, thymolphthalein solution 1ml / l.

[0012] (2) Get the Spirulina platensis in the logarithmic growth phase and inoculate it into the medium for cultivation, light for 10 hours every day, and the light intensity is about 50 μmol / (m 2 s), the temperature of the incubator is controlled at about 25 degrees Celsius, and 0.1% CO is introduced 2 air, the ventilation volume is 0.2vvm. Record the dry weight (DW) of the spirulina in the culture medium, the pH value of the algae liquid and the color of the algae liquid at regular interv...

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Abstract

The invention discloses a spirulina culture medium. A formula of the spirulina culture medium is prepared from the following components in mass concentration: 15 to 18g / L of sodium bicarbonate, 0.3 to 0.8g / L of superphosphate, 0.3 to 0.8g / L of potassium chloride, 0.5 to 1.5g / L of sodium chloride, 0.1 to 0.3g / L of magnesium chloride, 10 to 20mg / L of ammonium ferric citrate, 10 to 12mg / L of N2EDTA (Ethylene Diamine Tetraacetic Acid), 0.005 to 0.007mg / L of VB1, 0.04 to 0.06mg / L of VB12, 20 to 26mcg / L of ZnSO4.4H2O, 160 to 185mcg / L of MnCl2.4H2O, 8 to 12mcg / L of CuSO4.5H2O8, 6.8 to 7.5mcg / L of Na2MoO4.2H2O, 10 to 14mcg / L of CoCl2.6H2O, 0.5 to 1mg / L of plant growth regulating substances, and 0.5 to 1.5ml / L in volume concentration of a spirulina pH indicator. The spirulina culture medium in spirulina culture has the advantages of being high in survival rate and high in spirulina growth rate; in addition, the pH of the spirulina liqluid is conveniently regulated, so that the culture efficiency can be increased.

Description

technical field [0001] The invention relates to an algae culture medium, in particular to a spirulina culture medium. Background technique [0002] Spirulina is a blue photoautotrophic microscopic algae belonging to Cyanophyta, Oscillatoriaceae, and Spirulina. The protein content of spirulina is as high as 60-70%. It is an ideal protein recommended by the Food and Agriculture Organization of the United Nations (FAD). It contains eight kinds of amino acids that the human body cannot synthesize by itself, including lysine, threonine, methionine, Amino acid, leucine, tryptophan, phenylalanine, and valine, whose content in spirulina is very close to the human intake recommended by the FAD organization, can be reasonably supplemented and fully absorbed and utilized. In addition, spirulina is also rich in vitamins, algae polysaccharides, lipid compounds, carotene, chlorophyll, minerals, and phycocyanin. Natural spirulina has very little cell wall structure fiber, so its nutrients...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 王兆伟彭小伟王洁宋佳巍杜学星江灵芝孙小雪
Owner NINGBO FUTIAN BIOTECH