Preparation method of bio-modified castor protein and application of bio-modified castor protein to preparation of drug for treating malignant pleural mesothelioma

A biological modification, protein technology, applied in antitumor drugs, peptide/protein components, medical preparations containing active ingredients, etc., to achieve the effects of stable quality, diverse dosage forms, and obvious curative effects

Inactive Publication Date: 2015-05-20
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] Experiments have proved that the ricin toxin that binds A chain and B chain with disulfide bonds is very toxic, but the separated A chain and B chain are not t

Method used

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  • Preparation method of bio-modified castor protein and application of bio-modified castor protein to preparation of drug for treating malignant pleural mesothelioma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Put 1000g of dried biomodified castor bean body into a room temperature puffing device (200520108339.X), fill the puffing device with carbon dioxide gas and pressurize it to 0.1~5MPa for 10~60min, and then release it quickly Pressure to normal pressure, the biomodified castor bean body is expanded into powder;

[0035] (2) The bio-modified castor-bean powder after puffing and pulverization is added to the expansion solvent system extraction device (201210179076.6), and 1~10Kg of liquid carbon dioxide, 100~500mL of methanol and 100~300g of NH 3 ·H 2 O, pressurize to 2~10MPa to form an expansion solvent system, keep the temperature at 10~20°C for 10~30min, dissolve the protein in the biomodified castor bean powder, and then reduce the pressure to normal pressure to generate biomodified castor Crude hemp protein with methanol-NH 3 ·H 2 O mixture;

[0036] (3) Collect the solution containing the crude biomodified castor protein, and filter it with a microporous filt...

Embodiment 2

[0042] (1) Put 1000g of dried biomodified castor bean body into a room temperature puffing device (200520108339.X), fill the puffing device with carbon dioxide gas and pressurize it to 0.1~5MPa for 10~60min, and then release it quickly Pressure to normal pressure, the biomodified castor bean body is expanded into powder;

[0043] (2) The bio-modified castor powder after puffing and crushing is added to the expansion solvent system extraction device (201210179076.6), and 5Kg of liquid carbon dioxide, 300mL of methanol and 200g of NH 3 ·H 2 O, pressurize to 5MPa to form an expansion solvent system, keep the temperature at 15°C for 20 minutes, dissolve the protein in the biomodified castor bean powder, then reduce the pressure to normal pressure, and generate the biomodified castor protein crude product and methanol- NH 3 ·H 2 O mixture;

[0044] (3) Collect the solution containing the crude biomodified castor protein, and filter it with a microporous filter with a pore size of...

Embodiment 3

[0050] (1) Put 1000g of dried biomodified castor bean body into a room temperature puffing device (200520108339.X), fill the puffing device with carbon dioxide gas and pressurize it to 0.1~5MPa for 10~60min, and then release it quickly Pressure to normal pressure, the biomodified castor bean body is expanded into powder;

[0051] (2) The bio-modified castor-bean powder after puffing and crushing is added to the expansion solvent system extraction device (201210179076.6), and 1~10Kg of liquid carbon dioxide, 100~500mL of ethanol and 100~300g of ammonium chloride are added, and the pressure is increased to 2~ 10MPa to form an expansion solvent system, keep the temperature at 10~20°C for 10~30min, dissolve the protein in the biomodified castor bean powder, and then reduce the pressure to normal pressure to generate biomodified castor protein crude product and ethanol-chlorine A mixture of ammonium chloride;

[0052] (3) Collect the solution containing the crude biomodified casto...

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Abstract

The invention discloses a preparation method of a bio-modified castor protein and application of the bio-modified castor protein to preparation of a drug for treating malignant pleural mesothelioma. The preparation method of the bio-modified castor protein comprises the following steps of puffing and smashing a bio-modified castor insect body in a normal-temperature puffing device; then, adding the smashed bio-modified castor insect body into an expanding solvent system extracting device to extract a crude bio-modified castor protein; collecting a solution containing the crude bio-modified castor protein, and filtering by using a micropore filter to remove impurities; carrying out ultrafiltration treatment by using an ultrafilter, and collecting a bio-modified castor protein ultrafiltration solution; dissolving the ultrafiltration solution into acetonitrile, separating by using a solid-phase extraction column, and collecting an eluted component; dissolving the eluted component into a solvent system, and separating and purifying by using an ultrasonic stationary wave preparative liquid chromatography; and preparing freeze-dried protein powder by using a vacuum freeze-drying machine, wherein the freeze-dried protein powder can be used for preparing the drug for treating malignant pleural mesothelioma. A bio-modified castor protein preparation prepared by using the preparation method is stable in quality, clear in structure, distinct in pharmacology and remarkable in curative effect.

Description

technical field [0001] The invention relates to a preparation method of medicine and its new application, in particular to a preparation method of biomodified castor protein and its application in preparation of anti-malignant pleural mesothelioma medicine. Background technique [0002] Malignant pleural mesothelioma is a pleural tumor originating from the pleural mesothelial tissue. About 80% of patients with malignant pleural mesothelioma are related to exposure to various asbestos fibers in the occupational environment. Other possible factors leading to malignant pleural mesothelioma include tuberculous pleurisy, viral infection, radiation exposure, history of exposure to special industrial compounds, and genetic factors etc. [0003] Currently, the commonly used treatment methods include surgery, radiotherapy, chemotherapy, immunotherapy and molecular targeted therapy. [0004] Traditional Chinese medicine treats the symptoms by promoting qi and activating blood circul...

Claims

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Application Information

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IPC IPC(8): C07K1/36C07K1/34C07K1/16A61K38/00A61K9/02A61K9/127A61K9/48A61P35/00A61K36/47A61K35/64
Inventor 徐华民赵荣华
Owner HARBIN INST OF TECH
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