Microbial preparation for reclamation soil of gangue hill and method for reclamation of coal gangue soil using the preparation

A technology of microbial preparations and coal gangue, which is applied in the field of gangue reclamation, can solve the problems of unfavorable accumulation of organic matter in gangue, low number of soil microorganisms, poor nutrients in gangue, etc., and achieve the goal of solving the problem of spontaneous combustion, low cost, and reduced effectiveness Effect

Active Publication Date: 2018-12-21
INST OF AGRI ENVIRONMENT & RESOURCE SHANXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, it is generally used in production to "discharge coal gangue in layers, cover soil and roll it", and then carry out vegetation construction, but practice has proved that this planting mode of covering soil still has many defects: 1) gangue hills are poor in nutrients, and crops are difficult to grow; 2) The gangue hill has a high ground temperature, a small number of soil microorganisms, and a simple microbial community, which is not conducive to the accumulation of organic matter in the gangue hill; 3) After the gangue hill is covered with soil and rolled, there will still be oxygen, which will cause the gangue hill to spontaneously combust

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] There are six microbial strains involved in the preparation of microbial preparations, namely Bacillus subtilis ACCC10242, Bacillus licheniformis ACCC01193, Bacillus megaterium ACCC11107, Bacillus mucilaginosus ACCC10013, Azotobacter chroococcum ACCC10006, and Lysinibacillus fusiformis CGMCC1.3602 are all freeze-dried strains and are commercial products.

[0026] Under aseptic conditions, the target strains were successively activated, cultured on slant planes, cultured in liquid shake flasks and fermenters.

[0027] 1) Activation. Under aseptic conditions, clean the unstrained spirits tube containing freeze-dried and preserved microbial strains with absorbent cotton soaked in 75% alcohol, heat the top of the unstrained spirits tube with a flame, drop a small amount of sterile water to the heated top to make it rupture, Use tweezers to knock open the cracked ammonium tube. Use a sterile pipette to absorb 0.3-0.5mL of a suitable liquid medium, drop it into the fermente...

Embodiment 2

[0032] Weigh 50g of Bacillus subtilis sludge and 50g of Bacillus licheniformis sludge, mix with 150g of diatomaceous earth and 150g of bentonite, stir evenly with a stirrer, and place in a sterile ventilated room at 15-50°C to ventilate and dry until the water content reaches 2×10 8 CFU / g.

[0033] Weigh 50g of jelly-like Bacillus slime and 75g of Bacillus licheniformis slime, mix with 175g of diatomite and 200g of bentonite, add 75g of calcium magnesium phosphate fertilizer, stir evenly with a stirrer, and place in a sterile ventilated room at 15-50°C. Ventilate and dry in the room until the water content is less than 10%, to obtain type II microbial preparations, and the effective number of viable bacteria is greater than 2×10 8 CFU / g.

[0034] Weigh 25g of brown nitrogen-fixing bacteria sludge, 25g of Bacillus megaterium sludge, 50g of jelly-like Bacillus sludge, and 25g of Bacillus lysine sludge, mix with 150g of straw powder, 100g of sawdust, and 125g of peat soil, and...

Embodiment 3

[0036] Weigh 50g of Bacillus subtilis sludge and 75g of Bacillus licheniformis sludge, mix with 200g of diatomaceous earth and 175g of bentonite, stir evenly with a stirrer, and place in a sterile ventilated room at 15-50°C to ventilate and dry until the water content reaches 2×10 8 CFU / g.

[0037] Weigh 75g of jelly-like Bacillus slime and 75g of Bacillus licheniformis slime, mix with 200g of diatomite and 250g of bentonite, add 90g of calcium magnesium phosphate fertilizer, stir evenly with a stirrer, and place in a sterile ventilated room at 15-50°C Ventilate and dry in the room until the water content is less than 10%, to obtain type II microbial preparations, and the effective number of viable bacteria is greater than 2×10 8 CFU / g.

[0038] Weigh 50g of brown nitrogen-fixing bacteria sludge, 75g of Bacillus megaterium sludge, 125g of jelly-like Bacillus sludge, and 100g of Bacillus lysine sludge, mix with 350g of straw powder, 350g of sawdust, and 350g of peat soil, a...

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Abstract

The invention discloses a microbial preparation for gangue reclaimed soil. The microbial preparation comprises three microbial preparations which are respectively used independently, wherein the I type microbial preparation contains bacillus subtilis and bacillus licheniformis; the II type microbial preparation contains bacillus mucilaginosus and the bacillus licheniformis; the III type microbial preparation contains azotobacter chroococcum, bacillus megaterium, the bacillus mucilaginosus and lysine bacillus; the total viable count of every gram of the microbial preparation is (2-200)*10<8> CFU. The gangue reclaimed soil is covered in layers by using the microbial preparation disclosed by the invention, a first gangue layer, a sticky loess and fly ash mixing covering layer, a second gangue layer, a fly ash covering layer, a fine granular gangue layer and a loess covering layer are sequentially arranged from bottom to top, the I type microbial preparation is applied to the sticky loess and fly ash mixing covering layer, the II type microbial preparation is applied to the fly ash covering layer, the III type microbial preparation is applied to the loess covering layer, and crops are planted on the loess covering layer.

Description

technical field [0001] The invention belongs to the technical field of waste rock reclamation, and in particular relates to a special microbial preparation for waste rock reclamation soil and its application on coal mine waste rock. Background technique [0002] Coal gangue is the waste produced during the coal mining process, accounting for about 20% of the coal mining volume. At present, the comprehensive utilization rate of coal gangue in my country is about 30-40%, and most of them are piled up nearby as waste, forming gangue hills of different sizes, occupying land and destroying the landscape. Gangue hills have become the area with the worst site conditions, the largest stacking volume, and the most difficult governance in the coal mining area. There are more than 5,000 gangue dumps in the country, and about 1 / 3 of them spontaneously combust to varying degrees. Spontaneous combustion of coal gangue directly affects its reclamation effect and poses a certain threat to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05G3/00C12R1/125C12R1/10C12R1/07C12R1/11
CPCC05B17/00C05G3/00C12N1/20
Inventor 郜春花李建华张强靳东升卢朝东卢晋晶董云中
Owner INST OF AGRI ENVIRONMENT & RESOURCE SHANXI ACAD OF AGRI SCI
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