High-efficiency Microflow Electrochromatographic Separation Method for Carboxylated Plant Growth Regulators

A plant growth regulator and microfluidic chromatography technology, applied in the field of analytical chemistry, can solve the problems of retention and weak hormone retention, and achieve the effect of modification and change of the mechanism of action

Active Publication Date: 2016-06-01
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the separation of plant growth regulators by capillary electrochromatography is still mainly based on the reversed-phase chromatographic separation and electric field-driven migration of organic polymers on the surface of traditional capillary packing columns. Carboxylic acid phytohormones have weak retention and need to be improved

Method used

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  • High-efficiency Microflow Electrochromatographic Separation Method for Carboxylated Plant Growth Regulators
  • High-efficiency Microflow Electrochromatographic Separation Method for Carboxylated Plant Growth Regulators
  • High-efficiency Microflow Electrochromatographic Separation Method for Carboxylated Plant Growth Regulators

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Based on the capillary electrochromatography system, the octadecyl bonded silica gel capillary packed column is used for separation. The specific method is: apply a negatively charged high-voltage electric field at the end of the capillary column and apply a liquid phase pump auxiliary pressure on the injection port, and the injection volume is 20 μL , with acetonitrile: sodium chloride solution = 70: 30 (v / v) as the mobile phase, the concentration of sodium chloride is 3.0mmol / L, the separation voltage is +8.0kV, the pump auxiliary pressure is 1000psi, the flow rate is 0.05mL / min, and the plant growth The elution peaks of the modulators are: 2,4a,7-trihydroxy-1-methyl-8-methylene gibberal-3-ene-1,10-dicarboxylic acid-1,4a-lactone (peak 1); 2-cis,4-trans-5-(1-hydroxy-4-oxo-2,6,6-trimethyl-2-cyclohexen-1-yl)-3-methyl -2,4-Nadienoic acid (peak 2); 2,4-dichlorophenoxyacetic acid (peak 3).

Embodiment 2

[0025] Based on the capillary electrochromatography system, the octadecyl bonded silica gel capillary packed column is used for separation. The specific method is: apply a negatively charged high-voltage electric field at the end of the capillary column and apply a liquid phase pump auxiliary pressure on the injection port, and the injection volume is 20 μL , using acetonitrile: buffer solution = 70:30 (v / v) as the mobile phase, the electrolytes in the buffer solution are ammonium formate, sodium bisulfate, sodium bisulfite, the concentration is 3.0mmol / L, the separation voltage + 8.0kV, pump auxiliary pressure 1000psi, flow rate 0.05mL / min, capillary electrochromatographic separation of plant growth regulators, when sodium bisulfite is used as the electrolyte of the mobile phase, the elution peaks are: 2, 4a, 7-three Hydroxy-1-methyl-8-methylenegibberal-3-ene-1,10-dicarboxylate-1,4a-lactone (peak 1); 2-cis,4-trans-5- (1-Hydroxy-4-oxo-2,6,6-trimethyl-2-cyclohexen-1-yl)-3-methy...

Embodiment 3

[0027] Based on the capillary electrochromatography system, the octadecyl bonded silica gel capillary packed column is used for separation. The specific method is: apply a negatively charged high-voltage electric field at the end of the capillary column and apply a liquid phase pump auxiliary pressure on the injection port, and the injection volume is 20 μL , using acetonitrile:buffer solution=70:30 (v / v) as the mobile phase, the electrolytes in the buffer solution are sodium sulfate, sodium bicarbonate, sodium chloride, the concentration is 3.0mmol / L, and the separation voltage is +8.0 kV, pump auxiliary pressure 1000psi, flow rate 0.05mL / min, capillary electrochromatographic separation of plant growth regulators, when sodium chloride is used as the electrolyte of the mobile phase, the elution peaks are: 2,4a,7-trihydroxy- 1-methyl-8-methylenegibberal-3-ene-1,10-dicarboxylate-1,4a-lactone (peak 1); 2-cis,4-trans-5-(1 -Hydroxy-4-oxo-2,6,6-trimethyl-2-cyclohexen-1-yl)-3-methyl-...

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Abstract

The invention discloses a high-efficiency micro-current chromatographic separation method for a carboxyl-containing plant growth regulator, based on a reversed-phase capillary column, the compound containing a nucleophilic group is used as a mobile phase electrolyte, and the reversed-phase column filler is dynamically modified to form a nucleophilic base The high-voltage electric field and micro-flow liquid phase analysis mode are applied to the column to achieve efficient separation of carboxyl-containing plant growth regulators. The invention conveniently realizes the combined effects of electroosmotic flow, electrophoresis and polar chromatography, and realizes capillary electrophoresis separation and nucleophilic group adsorption chromatography separation of polar carboxylic acid plant growth regulators.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to a high-efficiency micro-current chromatographic separation method for carboxyl-containing plant growth regulators. Background technique [0002] Plant growth regulators are synthetic chemical substances used to regulate plant growth and development. They are mainly divided into plant growth promoters, plant growth retardants, and plant growth inhibitors. Among them, 2,4a,7-trihydroxy-1-methyl -8-methylene gibberellin-3-ene-1,10-dicarboxylic acid-1,4a-lactone belongs to gibberellin growth hormone, 2-cis,4-trans-5-(1- Hydroxy-4-oxo-2,6,6-trimethyl-2-cyclohexen-1-yl)-3-methyl-2,4-pentadienoic acid is a sesquiterpene structure The plant hormone, 2,4-dichlorophenoxyacetic acid belongs to the auxin analogues and is widely used in production. [0003] Regarding the detection of carboxylic acid plant growth regulators, mainly high performance liquid chromatography, liqui...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 林旭聪王绪培谢增鸿
Owner FUZHOU UNIV
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