A kind of hyaluronic acid film and its application

A technology of hyaluronic acid and film, which is applied in the direction of material absorption and weighing, measurement/inspection of microorganisms, biochemical equipment and methods, etc., can solve problems such as complicated process, cumbersome solvent evaporation method, and film cannot be restored to its original state, and achieve Strong binding and large HA binding effect

Active Publication Date: 2016-11-30
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, hyaluronic acid films are prepared by the cross-linked gel method, but the adipic acid dihydrazide used in this method is a toxic substance, and the solvent evaporation method used is more cumbersome, and the prepared film cannot absorb water after swelling. Restore the original state; and the process of chitosan-gelatin-hyaluronic acid composite film preparation method is more complicated

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1) First place the chip in piranha solution (hydrogen peroxide H 2 o 2 : concentrated sulfuric acid H 2 SO 4 =3:7) After soaking for 1 hour, rinse with deionized water several times until the surface of the chip is completely clean, ensuring that there is no substance on the surface, and dry the chip with nitrogen. Soak the clean chip in 0.08mg / ml bBSA solution. After 2 hours, rinse the chip several times with deionized water to wash away the bBSA molecules that are not bound to the chip surface, and then dry the surface with nitrogen; then the chip Soak in 0.38mg / ml Neutravidin solution, bind Neutravidin to the bBSA layer through the mutual combination of Biotin-Neutravidin, take out the chip after 2 hours, and rinse the chip several times with deionized water to remove the non-binding bBSA Rinse off the Neutravidin on the surface, and dry the surface with nitrogen; finally soak the chip combined with bBSA and Neutravidin in 0.1 mg / ml biotin-HA solution, and also bi...

Embodiment 2

[0025] 1) Prepare HA chips adsorbed with different molecular weights by the above method;

[0026] 2) Use QCM to compare the effects of HA layers with different molecular weights and CD44, place the chip prepared in 1) into the liquid pool of QCM, pass into PBS buffer, find the fundamental frequency, wait until the frequency value signal is stable at 0, pass into 0.25μM CD44 Solution, observe the interaction between the two in real time, the flow rate is 50μl / min, after half an hour, the frequency value is stable, continue to pass through PBS buffer, wash away the CD44 that is not bound to the surface of HA, and observe the binding between HA and CD44 If the mutual binding between the two is strong, CD44 will not be washed away by PBS buffer, if the mutual binding is weak, CD44 will be washed away by PBS buffer;

[0027] 3) Comparing the interaction of HA membranes with different molecular weights with CD44, it was found that 2192kDa HA had a strong binding specificity to CD44...

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PUM

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Abstract

The invention provides a hyaluronic acid film and application thereof. The hyaluronic acid film is prepared by adopting a chemical adsorption method, the method is simple, and environment-friendly and non-toxic effects are achieved, the prepared film has good salt responsiveness and pH responsiveness, when a water environment is restored, the film can reset to be of the original shape, and the film can be repeatedly used. In addition, the film can be used for judging whether cells are cancerous to screen tumor cells. HA is chemically adsorbed on a gold chip by using a biotin-Neutravidin system, due to quick, dedicated and stable combination of the components, the HA combined on the chip is very firm, and the combined quantity is large; moreover, when QCM is used for researching the combination of HA with different molecular weights and CD44, the effect is good and is conductive to research on the combination mechanism.

Description

technical field [0001] The invention belongs to the technical field of application of bioactive materials, and in particular relates to a hyaluronic acid film and its application. Background technique [0002] Hyaluronic acid (Hyaluronic acid, HA) is a linear polysaccharide anionic polymer, composed of alternating repeating N-acetylglucosamine and D-glucuronic acid disaccharide unit links, with a molecular weight ranging from a few thousand to several million. HA has the characteristics of a relatively simple chemical structure, a large number of functional groups that can be modified, high water solubility, and the ability to combine up to 1000 times the volume of water to form a hydrogel or cross-link to form a smart polymer biomaterial. It has a long history of development and utilization. On the other hand, HA is the main component of the extracellular matrix, has special chemical properties, and participates in the biological functions of promoting cell adhesion, balanc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B32B7/04B32B9/00B32B33/00C12Q1/04G01N5/02
Inventor 姜磊韩娟李友训杨丽敏马洪超
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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