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Preparation method and applications of fluorescent carbon quantum dots

A technology of carbon quantum dots and fluorescence, which is applied in the field of preparation and application of fluorescent carbon quantum dots, can solve the problems of insufficient quantum dots and low quantum yield of carbon dots, and achieve simple preparation methods, low toxicity, and fluorescent quantum production. high rate effect

Inactive Publication Date: 2015-06-17
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

However, the quantum yield of carbon dots is still very low, not reaching the level of quantum dots. Under such a background, the preparation of carbon dots with high fluorescence quantum yield is particularly necessary.

Method used

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  • Preparation method and applications of fluorescent carbon quantum dots
  • Preparation method and applications of fluorescent carbon quantum dots
  • Preparation method and applications of fluorescent carbon quantum dots

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Embodiment 1

[0027] Preparation and characterization of carbon dots:

[0028] (1) Preparation and separation and purification of carbon dots:

[0029] First, take 0.9g of BSA, dissolve it in 20ml of deionized water, then add 0.318g of 88% formic acid drop by drop, and stir the mixture to make the mixture evenly mixed. , using a temperature program to raise the temperature for 1h, the temperature was raised to 200°C, and the reaction time was 10h. The obtained product was separated and purified by a Sephadex G-25 gel column to remove unreacted formic acid. The obtained carbon dot aqueous solution was a golden yellow liquid, and then the product was frozen. dry.

[0030] (2) Measurement of the quantum yield of carbon dots:

[0031] Choose quinine sulfate solution as reference sample, select formula as:

[0032]

[0033] In the formula Be the quantum yield QY%, A is the ultraviolet absorption value, I is the fluorescence integral emission intensity, is the refractive index of the so...

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Abstract

The present invention discloses a preparation method and applications of fluorescent carbon quantum dots (Carbon dots, short for CDs). According to the preparation method, formic acid and bovine serum albumin (BSA) are adopted as raw materials, a molar ratio of the amino of the BSA to the aldehyde of the formic acid is 1:1, hydrothermal synthesis is performed, the aldehyde of the formic acid and the amino of the BSA are subjected to condensation to generate carbon nitrogen double bond, the generated polymer having the carbon nitrogen double bond self-reacts to generate carbon quantum dots, and a water-based membrane and a dextran G-25 gel column are used to separate and purify. According to the present invention, the carbon quantum dots prepared through the method have characteristics of small particle size, good light stability, wide excitation and emission spectrum range, high fluorescence quantum yield, low toxicity, and environmental protection, and can be successfully used in biological cell imaging, wherein the yield of the carbon quantum dots is generally low in the prior art is solved with the preparation method of the present invention.

Description

technical field [0001] The invention relates to a fluorescent nanometer material, in particular to the preparation and application of a fluorescent carbon quantum dot. Background technique [0002] In recent years, with the rapid development of nanotechnology, nanomaterials (such as: nanomagnetic materials, nanoceramic materials, nanosensors, nanoscale functional materials, nanosemiconductor materials, and nanocatalytic materials) have been widely recognized and applied. In the fields of biology and medicine, the preparation and application of nanomaterials have gradually become more sophisticated, and quantum dots for biomarkers are one of them. Quantum dots, also known as nanocrystals, are nanoparticles composed of II-VI or III-V elements. The particle size is generally between 1 and 10nm. Since electrons and holes are quantum-confined, the continuous energy band structure becomes a discrete energy level structure with molecular characteristics, which can emit fluorescenc...

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Application Information

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IPC IPC(8): C01B31/02C09K11/65G01N21/64
Inventor 谭明乾李欣彤马小军
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI