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Application of Citrobacter Citrobacter in the Preparation of Bioflocculants by Recycling Waste Algae

A technology of biological flocculant and Citrobacter, which is applied in the direction of flocculation/sedimentation water/sewage treatment, bacteria, seawater treatment, etc. It can solve the problems of limited scale algae control and high cost of biological flocculants, and achieve high-efficiency flocculation dosage, good Flocculation effect, high safety effect

Inactive Publication Date: 2017-01-18
NORTHEAST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides an application of Citrobacter in the preparation of biological flocculant by recycling waste algae to solve the problems of high cost of biological flocculant and limited scale of algae control

Method used

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  • Application of Citrobacter Citrobacter in the Preparation of Bioflocculants by Recycling Waste Algae
  • Application of Citrobacter Citrobacter in the Preparation of Bioflocculants by Recycling Waste Algae
  • Application of Citrobacter Citrobacter in the Preparation of Bioflocculants by Recycling Waste Algae

Examples

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Embodiment 1

[0025] A preparation method of a bioflocculant of Citrobacter, comprising the following steps:

[0026] (a) Citrobacter is fermented and cultivated to obtain a fermented liquid, the medium of the fermented culture contains 1% glucose, the wet weight of algae cells is 5%, the rest is water, pH6.0; The weight is 0.5%, the culture temperature is 20°C, the shaking culture is 40 hours, the mycelium is removed by centrifugation, and the supernatant is obtained;

[0027] (b) Add 2 volumes of ethanol to the supernatant and store at 4°C for 12 hours. After the mixture is centrifuged, dissolve 3-5 g of the obtained precipitate in 100 ml of deionized water, and add 50 ml of 2% decachloride at the same time. Hexaalkylpyridine, shaken for 3 hours, after that, collect the precipitate and dissolve it in 100ml of 0.5M NaCl solution, add 2 times the volume of ethanol, centrifuge and collect the precipitate;

[0028] (c) The collected precipitate was eluted with ethanol, dissolved in 5ml of de...

Embodiment 2

[0034] Using the Citrobacter ( Citrobacter sp. AzoR-1 ) A method for preparing a bioflocculant, comprising the following steps:

[0035] (a) Citrobacter ( Citrobacter sp. AzoR-1 ) through fermentation to obtain a fermented liquid, wherein the culture medium of the fermentation contains 1.5% glucose, the wet weight of algae cells is 6.5%, the rest is water, pH7.0; the inoculum size is 1.2% based on the total weight of the fermentation medium, The culture temperature is 28°C, the shaking culture is carried out for 80 hours, and the mycelium is removed by centrifugation to obtain the supernatant;

[0036] (b) Add 2 times the volume of ethanol to the supernatant at 4°C for 12 hours. After the mixture is centrifuged, dissolve 4 g of the obtained precipitate in 100 ml of deionized water, and add 50 ml of 2% hexadecane chloride at the same time. Pyridine, shaken for 3.5 hours, after that, collect the precipitate and dissolve it in 100ml of 0.5M NaCl solution, add 2 times the volume...

Embodiment 3

[0043] Using the Citrobacter ( Citrobacter sp. AzoR-1 ) The method for preparing biological flocculant, is characterized in that, comprises the following steps:

[0044] (a) Citrobacter ( Citrobacter sp. AzoR-1 ) to obtain a fermented liquid through fermented culture, the culture medium of said fermented culture contains glucose 2%, the wet weight of algal cells is 8%, the rest is water, pH8.0; the inoculum size is 2% by the total weight of the fermented medium, The culture temperature is 35°C, the shaking culture is carried out for 120 hours, and the mycelium is removed by centrifugation to obtain the supernatant;

[0045] (b) Add 2 volumes of ethanol to the supernatant at 4°C for 12 hours, centrifuge the mixture, dissolve 5 g of the obtained precipitate in 100 ml of deionized water, and add 50 ml of 2% hexadecane chloride at the same time Pyridine, shaken for 4 hours, after that, collect the precipitate and dissolve it in 100ml of 0.5M NaCl solution, add 2 times the volume o...

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Abstract

The invention relates to the application of citric acid bacteria in the preparation of biological flocculant by recycling waste algae, which belongs to the method for treating algae blooms. Ferment and culture Citrobacter to obtain fermentation liquid, the inoculation amount is 0.5-2% based on the total weight of the fermentation medium, centrifuge to remove mycelium, and obtain supernatant; add 2 times the volume of ethanol to the supernatant at 4°C After 12 hours, after centrifuging the mixture, dissolve 3~5g of the obtained precipitate in 100ml of deionized water, add 50ml of 2% cetylpyridinium chloride at the same time, shake for 3~4 hours, after that, collect the precipitate and dissolve Add 2 times the volume of ethanol to 100ml of 0.5M NaCl solution, centrifuge and collect the precipitate; elute the collected precipitate with ethanol, dissolve it in 5ml of deionized water, and dry it in vacuum to obtain the product. The advantage is that the production cost of the bioflocculant is greatly reduced, and no secondary pollution is caused; it is easily degraded by microorganisms, non-toxic and harmless, and has high safety, which is conducive to improving the application and promotion of the bioflocculant.

Description

technical field [0001] The invention relates to a method for treating water blooms, in particular to a method for producing a biological flocculant capable of flocculating and removing algae by utilizing waste algae circulation through bacterial strains. Background technique [0002] Due to the loss of agricultural fertilizers, the discharge of domestic sewage, aquaculture and industrial sewage, and the burning of fossil fuels, a large amount of nutrients such as nitrogen and phosphorus enter lakes, estuaries, bays and other slow-flowing water bodies, resulting in eutrophication of water bodies. In eutrophic freshwater bodies, blue-green algae reproduce in large numbers under suitable conditions, forming a "bloom". The overgrowth of algae is a serious hazard and has become a global environmental problem. The large-scale outbreak of algae will reduce the dissolved oxygen in the water body, and even anoxic and anaerobic, resulting in the death of aquatic organisms and serious...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F1/52C12N1/20C02F103/08
Inventor 徐亮霍明昕霍铭远
Owner NORTHEAST NORMAL UNIVERSITY
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