Application of Citrobacter Citrobacter in the Preparation of Bioflocculants by Recycling Waste Algae

A technology of biological flocculant and Citrobacter, which is applied in the direction of flocculation/sedimentation water/sewage treatment, bacteria, seawater treatment, etc. It can solve the problems of limited scale algae control and high cost of biological flocculants, and achieve high-efficiency flocculation dosage, good Flocculation effect, high safety effect
CN104724807BInactive Publication Date: 2017-01-18NORTHEAST NORMAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NORTHEAST NORMAL UNIVERSITY
Publication Date
2017-01-18
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to the application of citric acid bacteria in the preparation of biological flocculant by recycling waste algae, which belongs to the method for treating algae blooms. Ferment and culture Citrobacter to obtain fermentation liquid, the inoculation amount is 0.5-2% based on the total weight of the fermentation medium, centrifuge to remove mycelium, and obtain supernatant; add 2 times the volume of ethanol to the supernatant at 4°C After 12 hours, after centrifuging the mixture, dissolve 3~5g of the obtained precipitate in 100ml of deionized water, add 50ml of 2% cetylpyridinium chloride at the same time, shake for 3~4 hours, after that, collect the precipitate and dissolve Add 2 times the volume of ethanol to 100ml of 0.5M NaCl solution, centrifuge and collect the precipitate; elute the collected precipitate with ethanol, dissolve it in 5ml of deionized water, and dry it in vacuum to obtain the product. The advantage is that the production cost of the bioflocculant is greatly reduced, and no secondary pollution is caused; it is easily degraded by microorganisms, non-toxic and harmless, and has high safety, which is conducive to improving the application and promotion of the bioflocculant.
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Description

technical field

[0001] The invention relates to a method for treating water blooms, in particular to a method for producing a biological flocculant capable of flocculating and removing algae by utilizing waste algae circulation through bacterial strains. Background technique

[0002] Due to the loss of agricultural fertilizers, the discharge of domestic sewage, aquaculture and industrial sewage, and the burning of fossil fuels, a large amount of nutrients such as nitrogen and phosphorus enter lakes, estuaries, bays and other slow-flowing water bodies, resulting in eutrophication of water bodies. In eutrophic freshwater bodies, blue-green algae reproduce in large numbers under suitable conditions, forming a "bloom". The overgrowth of algae is a serious hazard and has become a global environmental problem. The large-scale outbreak of algae will reduce the dissolved oxygen in the water body, and even anoxic and anaerobic, resulting in the death of aquatic organisms and serious...

Claims

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