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Rhodococcus and application of rhodococcus in degradation of lignin

A rhodococcus and lignin technology, applied in the field of microorganisms, can solve problems such as difficult degradation, environmental pollution, and chemical methods cannot solve pollution problems, and achieve low cost and no secondary pollution

Active Publication Date: 2015-06-24
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The combination of lignin with cellulose and hemicellulose is very stable and not easy to degrade. It is one of the key bottlenecks in the utilization of biomass energy. At the same time, lignin is also a by-product of the wood hydrolysis industry and paper industry and a relatively difficult to degrade in municipal solid waste. The use of chemical reagents to treat these wastes will cause serious environmental pollution
[0003]Although there have been decades of research on lignin degradation methods, there are still many problems, such as chemical methods cannot solve the pollution problem, and physical methods cannot There is a challenge of high energy consumption, and biodegradation is widely used in the degradation of various harmful organic substances due to its low cost, mild reaction conditions, and no secondary pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Rhodococcus ( Rhodococcus sp.) L17 was isolated and purified from sea mud in the South China Sea.

[0022] The separation and purification method is as follows: the acclimatization medium used is inorganic salt medium, and the composition is (g / L): 6.2g Na 2 HPO 4 , 3.0g KH 2 PO 4 , 0.5g NaCl, 1.0g NH 4 Cl, pH 7.0. First, add 3g of sludge to 100mL of inorganic salt medium containing 20mg / L lignin, and after acclimatization at 37°C for 60 days, insert 10% inoculum volume of lignin into inorganic salt medium containing 40mg / L of lignin, and then acclimate for one week, followed by By analogy, gradually increase the concentration of lignin for domestication, and the concentrations of lignin are 40mg / L, 60mg / L, and 80mg / L respectively.

[0023] After the acclimatization, spread the acclimatization solution on the agar plate (beef extract 3g / L, peptone 10g / L, sodium chloride 5g / L, agar powder 20g / L, pH7.0) and culture at 37°C for 48h, and select a single colony on Be...

Embodiment 2

[0034] Rhodococcus ( Rhodococcus sp.) L17 single colony was inoculated in 3mL beef extract peptone medium, cultured for 18h (OD 600 0.45) with an inoculum volume of 20% by volume, insert it into an inorganic salt medium with a lignin concentration of 20mg / L and a pH of 8.0, react at 37°C with a shaker at 150rpm for 96h, and measure the degradation by UV spectrophotometry Rate. The mensuration of degradation rate is the same as embodiment 1, and detection result is that degradation rate is 78%.

Embodiment 3

[0036] Rhodococcus ( Rhodococcus sp. .) A single colony of L17 was inoculated in 3 mL of beef extract peptone medium, and the 18-hour culture was inserted into an inorganic salt medium with a lignin concentration of 60 mg / L and a pH of 7.0 at an inoculation volume of 40% by volume. ℃, 100rpm shaker reaction for 108h, the degradation rate was measured by ultraviolet spectrophotometry, the measurement method was the same as in Example 1, and the test result was that the degradation rate was 82%.

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Abstract

The invention relates to a rhodococcus and application of the rhodococcus in degradation of lignin, belonging to the technical field of microorganism. The rhodococcus is obtained from China South Sea mud through separation and purification, is a variety of rhodococcus, and is named (Rhodococcus sp.) L17, the rhodococcus is Gram-positive according to authentication, no conidium is formed, and the rhodococcus is aerobic, is catalase positive, and is aryl-sulfatidase negative; besides, the 16SrDNA sequence of the rhodococcus is submitted to the NCBI database, the accession number is KM215139, and is preserved in the general microorganism center of CCCCM (China Committee for Culture Collection of Microorganisms) on March 20th in 2014 with the preservation number CGMCC8943; according to verification of experiments, the rhodococcus has the capacity of degrading the lignin, and the degradation rate can reach 90%-100%, so that the rhodococcus can be applied to the field of environment protection, and can be particularly applied to biodegradation of the lignin.

Description

Technical field [0001] The invention involves a kind of red bacteria and its application in the quality of degradation, which is the field of microbial technology. Background technique [0002] Lyrin is one of the three major biomass in nature in nature, accounting for 15%-40%of the dry weight of terrestrial plants. The structure is a three-dimensional spatial structure of the phenylphenyne unit structure.The aromatherapy compounds, the main component of the plant skeleton composition of the cellulose and semic cellulose.The combination of lignin and cellulose and semi -cellulose is very stable, and it is not easy to degrade. It is one of the key bottlenecks for the use of biomass energy. At the same time, lignin is also a by -product of the wood hydrolysis industry and the papermaking industry.Materials, using chemical reagents to treat these wastes can cause serious environmental pollution. [0003] Although there have been decades of research on the degradation method of ligni...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P7/64C12R1/01
CPCC12N1/205C12R2001/01
Inventor 朱道辰孙建中谢长校李成林张佩佩
Owner JIANGSU UNIV
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