Bisphenol A sensor of nano-porous gold modified based on aptamer and method

A nanoporous gold and aptamer technology, applied in the field of electrochemical detection, achieves the effects of low cost, high stability, high reproducibility and high detection accuracy

Active Publication Date: 2015-07-01
SHANDONG UNIV
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  • Abstract
  • Description
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Problems solved by technology

However, the combination of NPG with excellent electrocatalysis and specific biomolecular recognition elements for the design of highly sensitive and selective electrochemical sensors for the detection of BPA has never been reported so far.

Method used

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  • Bisphenol A sensor of nano-porous gold modified based on aptamer and method
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  • Bisphenol A sensor of nano-porous gold modified based on aptamer and method

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Embodiment 1

[0029] 1. Experimental part

[0030] 2.1 Chemical reagents and materials

[0031] BPA was purchased from Aladdin Reagent Co., Ltd. (Shanghai, China). A 5-thiolated BPA aptamer that can specifically recognize BPA (5'–CCG GTG GGT GGT CAG GTG GGA TAG CGT TCC GCG TAT GGC CCA GCG CAT CAC GGG TTC GCA CCA–3')[31] Synthesized by Invitrogen Biotechnology Co., Ltd. (China, Beijing). Human serum samples were collected from healthy volunteers. PBS is prepared from 0.01M disodium hydrogen phosphate, 0.01M sodium dihydrogen phosphate and 0.9% sodium chloride solution. TE buffer (pH 8.0) made of 10 mM tris (trishydroxymethyl)aminomethane and 1 mM ethylenediaminetetraacetic acid was used to dilute the aptamers. Gold-silver alloy film (100nm thick; gold-silver mass ratio 50:50) was purchased from Changshu Precious Metals Company. All other chemical reagents were of analytical grade and used without further purification. Ultrapure water with a resistivity of 18.25 MΩ.cm was used to config...

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Abstract

The invention discloses a bisphenol A sensor of nano-porous gold modified based on aptamer and a method. The sensor and the method are used for performing direct electrochemical detection in high sensitivity and high selectivity on the bisphenol A (BPA). A gold-silver alloy film is placed in concentrated nitric acid to corrode the silver to obtain an NPG film. The NPG is used for modifying a glassy carbon electrode, and then modifying the aptamer of the BPA to manufacture the sensor. The NPG represents excellent electrochemical catalysis activity to the redox reaction of the BPA, thereby guaranteeing the high sensitivity of the sensor. The aptamer fixed on the NPG is capable of greatly prompting the selectivity of the sensor, the BPA captured by the aptamer displays the redox peak at 0.35V/0.28V (with respect to silver/silver chloride electrode). The experiment optimizes the important experimental parameter. The linear detection range of the detection method is 0.1nM to 100nM, and the detection limit is 0.056+/-0.004nM. The most noteworthy is that the sensor can be successfully applied to the detection of BPA in a human serum sample.

Description

technical field [0001] The invention belongs to the field of electrochemical detection, in particular to a bisphenol A sensor and method based on aptamer-modified nanoporous gold. Background technique [0002] Bisphenol A (BPA) is an important organic monomer in the synthesis of polycarbonate polymers and epoxy resins, which is widely found in food storage and packaging materials, including baby bottles, drinking water bottles, tableware And the internal coating of food cans, etc. With the widespread application and infiltration of BPA in the food and environmental fields [1], people have concerns about the harmfulness of BPA [2] [3]. In fact, BPA is a harmful endocrine-disrupting substance, because its structure is similar to hormones, it can pretend to be estrogen and interfere with the normal combination between estrogen and estrogen receptors [3], thus leading to disorders of the human endocrine system, Especially for infants and pregnant women will produce obvious har...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/26
Inventor 朱烨闫妍周楚青闫旭鹏
Owner SHANDONG UNIV
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